Expression of piezo receptors in the human TG and DRG.

<p>FAM38A and B are highly expressed in both sensory ganglia.</p

The OR6B2 protein is localized to satellite cells in human DRG.

<p>Immunohistochemical staining of human DRG sections with the OR6B2 antibody indicated OR6B2 protein expression in the neuron-surrounding satellite cells. DAPI staining (blue) was used to determine the number and localization of cell nuclei. The secondary antibody alone did not produce any staining (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128951#pone.0128951.s004" target="_blank">S4 Fig</a>). Scale bars: 10 μm.</p

Expression of ACCN in the human TG and DRG.

<p>ACCNs 1–3 are highly expressed in sensory ganglia.</p

Expression of MRGPRs in the human TG and DRG.

<p><b>A</b> MRGPR transcripts were detected in the TG and DRG, whereas they were mostly absent in the reference tissues investigated (brain, colon, liver, lung, s. muscle, and testis). Only MRGPRF transcripts could be detected in all reference tissues. NO = no ortholog <b>B</b> Investigation of the sFPKM values of all MRGPRs with the exception of MRGPRF in all tissues. The expression of MRGPRs is restricted to the sensory ganglia investigated.</p

<i>ros1</i> deletion strains fail to aggregate and form spores.

<p>Tumor samples from maize seedlings infected with the wild type strains FB1 x FB2 and the corresponding <i>ros1</i> deletion strains were collected at 4, 6, 8, 10, 12 dpi, stained with wheat germ agglutinin-Alexa Fluor 488 and analyzed by laser scanning confocal microscopy (bar = 50 μm). In the FB1 x FB2 infection (left column), spore formation starts at 6 dpi with the formation of hyphal aggregates which expand (8 dpi), begin to fragment (10 dpi) and develop into teliospores (12 dpi). In comparison, the <i>ros1</i> deletion strains are locked in the filamentous state, do not aggregate and do not develop teliospores (right column). The size bar corresponds to 50 μM.</p

Expression of FPRs in the human TG and DRG.

<p>Expression of FPR transcripts could be detected in human (TG and DRG) and murine (mTG and mDRG) sensory ganglia and other reference tissues (brain, colon, liver, lung, s. muscle, and testis).</p

Expression analysis of the 30 most selectively expressed ion channels in the human TG and DRG.

<p>A The heatmap shows the 30 most selectively expressed ion channels in the TG compared with the DRG and reference tissues (brain, colon, liver, lung, s.muscle, and testis). Genes are ranked according to their selective expression in the TG (Quotient of the mean FPKM value (TG1-4) and the mean FPKM value of all reference tissues, only those genes expressed in all four TG with FPKM >0.1 were taken into account), and of which SCN10A is the most selectively expressed ion channel in the human TG. B Shown are the 30 most selectively expressed ion channels in the DRG. The genes are ranked according to their selective expression in the DRG. The ranking was calculated by the quotient of the FPKM value in the DRG (FPKM >0.1) and the mean FPKM value for all reference tissues. SCN10A is the most selective ion channel detected in the DRG.</p

Expression of P2XRs in the human TG and DRG.

<p>The ionotropic purinergic receptors are highly expressed in sensory ganglia. Five of the seven P2XRs are expressed higher in the TG and DRG than in any other tissue.</p

Expression of KCNK channels in the human TG and DRG.

<p>All KCNK genes are expressed in the TG and DRG. The KCNK genes are sorted by the mean of their expression values across all human sensory ganglia.</p

Analysis of highly expressed OR genes in the human TG and DRG.

<p><b>A</b> Schematic representation of the newly identified 5’UTRs of OR6B3 and OR6B2. The gene is indicated by blue bars (exon) and thin lines (intron). The coding exon is indicated by ORF (open reading frame) and splice junctions as red arcs. We detected two unannotated 5’UTRs for OR6B3 and OR6B2. <b>B</b> 5’UTR-validation of OR-transcripts using RT-PCR with intron-spanning primers in the DRG sample. The newly identified 5’UTRs of the OR transcripts were confirmed by RT-PCR with a forward primer located in the identified exon and a reverse primer located in the ORF of the respective OR. OR6B3: Ex1 (forward primer in exon 1 of 5’UTR and reverse in OR6B3 ORF); Ex2 (forward primer in exon 2 of 5’UTR and reverse primer in OR6B3 ORF). OR6B2: Ex1 (forward primer in exon 1 of 5’UTR and reverse primer in OR6B2 ORF); Ex2 (forward primer in exon 2 of 5’UTR and reverse primer in OR6B2 ORF). The amplified PCR products were confirmed by Sanger sequencing.</p