Supplemental material for Environmental enrichment prevents pup mortality in laboratory mice

<p>Supplemental material for Environmental enrichment prevents pup mortality in laboratory mice by Charlotte S Leidinger, Christa Thöne-Reineke, Nadine Baumgart and Jan Baumgart in Laboratory Animals</p

Course of body weight.

<p>Data are means ± standard error. (A) Control ♀: n = 6, single anesthesia ♀: n = 13, repeated anesthesia ♀: n = 13. (B) Control ♂: n = 7, single anesthesia ♂: n = 13, repeated anesthesia ♂: n = 13. p values were calculated using repeated measures ANOVA with group as between-subject factor: * p < 0.05, ** p < 0.01, *** p < 0.001 versus 14 days after the last anesthesia; ^# p < 0.05 versus 9 days after the last anesthesia.</p

Latency to explore in the free exploratory paradigm for trait anxiety-related and exploratory behavior.

<p>Latency to explore [s]; data are means ± standard error; p values were calculated using the Kruskal-Wallis-Test: ** p < 0.01 versus repeated anesthesia. (A) Control ♀: n = 6, single anesthesia ♀: n = 13, repeated anesthesia ♀: n = 13. (B) Control ♂: n = 7, single anesthesia ♂: n = 13, repeated anesthesia ♂: n = 13.</p

Phases of anesthesia.

<p>Phases of anesthesia.</p

Burrowing behavior in the immediate postanesthetic period.

<p>Data are means ± standard error. p values were calculated using Kruskal-Wallis-Test: * p < 0.05. (A) Control ♀: n = 6, single anesthesia ♀: n = 9, repeated anesthesia ♀: n = 13; 4 mice of the single anesthesia group were excluded from statistics because of technical malfunctions of the scale. (B) Control ♂: n = 6, single anesthesia ♂: n = 10, repeated anesthesia ♂: n = 13; 3 mice of the single anesthesia group and 1 control mouse were excluded from statistics because of technical malfunction of the scale.</p

Mouse Grimace Scale difference scores at 30 min and 150 min after last anesthesia.

<p>MGS, Mouse Grimace Scale. Data are mean ± standard error. p values were calculated using Kruskal-Wallis-Test: ** p < 0.01. (A) Control ♀: n = 6, single anesthesia ♀: n = 9, repeated anesthesia ♀: n = 13; 4 mice of the single anesthesia group were excluded from statistics because of technical malfunction of the camera. (B) Control ♂: n = 6, single anesthesia ♂: n = 10, repeated anesthesia ♂: n = 13; 3 mice of the single anesthesia group and 1 control mouse were excluded from statistics because of technical malfunction of the camera.</p

Vital parameters.

<p>Vital parameters.</p

Food intake.

<p>Food intake.</p

A fractured keel bone with callus formation.

<p>The arrow shows the fracture callus.</p

Number of mice showing excitations, opisthotonus, and twitches.

<p>Number of mice showing excitations, opisthotonus, and twitches.</p