Genes involved in sexual development that are upregulated in <i>T. reesei</i> during growth on lactose.

*<p>fold change.</p

The lactose-induced CAZome of <i>T. reesei.</i>

<p>The lactose-induced CAZome of <i>T. reesei.</i></p

Figure 2

<p>(a) Growth comparison of QM 9414 to the Δ3405 strain on minimal media supplemented with D-glucose, lactose and D-galactose. Pictures were taken on day 4. (b-g) Comparison of strains QM 9414 (grey), Δ3405 (white) and <i>ptef::3405</i> (black) pregrown in minimal medium supplemented with 1% glycerol and,after washing of the mycelium extensivly with sterile water, transferred to minimal medium containing 1% lactose. (b) Accumulation of <i>cel6a</i>, <i>cel7a</i> and <i>xyr1</i> transcripts measured by qPCR. Samples were taken 5, 8, 16 and 24 h after replacement to 1% lactose. The expression is given in relation to <i>tef1</i> gene expression, where the <i>tef1</i> expression value equals one. Mean values ± SD of three independent experiments are shown. (c) Lactose concentration in the medium during growth of QM 9414 (filled diamonds), Δ3405 (empty circles) and <i>ptef::3405</i> (empty squares) on 1% lactose. (d) Biomass formation of strains QM 9414 (filled diamonds), Δ3405 (empty circles) and <i>ptef::3405</i> (empty squares) on 1% lactose. Mean values ± SD of three independent experiments are given. (e) SDS-PAGE analysis of culture filtrates from QM 9414, Δ3405 and <i>ptef::3405.</i> Samples taken at 16 and 24 h after replacement to lactose. (f) ß-galactosidase activity determined with <i>o</i>-nitrophenyl-ß-D-galactopyranoside as the substrate. Error bars indicate the standard deviation of three independent experiments.</p

Balance of significantly^* regulated transcripts during growth of <i>T. reesei</i> on lactose.

*<p>either>or <2-fold vs. glucose and glycerol.</p>*<p>%-values refer to the total number of genes.</p>**<p>%-values refer to the category of genes (e.g. identified, unknown or unique, resp.).</p

Major changes in the transcriptome during cultivation of <i>T. reesei</i> on lactose.

<p>Data on grey background were statistically significant (p<0.05). *N, not calculated because of zero in one condition.</p

Growth of several MFS-knock out strains on D-glucose, lactose, D-galactose, and where appropriate, D-xylose, and L-arabinose.

<p>Pictures were taken 96 h after of incubation on minimal medium supplemented with the appropriate carbon source (1% w/v).</p

Phylogenetic analysis of the putative lactose permease Trire2:3405.

<p>Phylogenetic analysis of the putative lactose permease Trire2:3405.</p