TGF-β signaling pathway.

<p>Genes highlighted in yellow are predicted targets of miR differentially regulated in N v TSI (35 miR) (FC>3 p<0.05). Genes highlighted in green are not predicted targets.</p

Venn diagram of differentially expressed miR.

<p>Venn diagram showing the number of differentially expressed (FC>3, p<0.05) miR that are unique or that overlap between the different clinical phenotypes. Selected miR of interest are shown with arrows illustrating whether they are up- or down-regulated in the indicated comparison group. An upward facing arrow indicates up-regulation and a downward facing arrow indicates down-regulation. * miR-23b is up-regulated in N v TS but down-regulated in the TS v TSI comparison group.</p

Sample demographic details before and after quality control exclusion for full array analysis.

<p>FPC grading scores (0–3) are shown for each phenotypic group.</p><p>Footnote: Age ranges between phenotypic groups are not significantly different within and between A and B genecard groups (Wilcoxon test p>0.05). Fewer B genecards were passed filtering as these cards were designed to cover less abundant miR.</p

Network co-expression analysis.

<p>Clusters of co-expressed miR are shown, calculated from normalized array data irrespective of phenotype. Clusters are highlighted according to the pathway most enriched upon pathway analysis of miR in each cluster.</p

Number of differentially expressed miR in array results.

*<p>p-values were not adjusted or controlled by false discovery rate. We calculated 1518 independent tests of significance on the entire array data set and estimate that this would result in a false positivity rate of 50% when accepting unadjusted p>0.05.</p

Results of qPCR differential expression analysis.

*<p>Unadjusted p-values are presented. With no inflation of p-values the chance of finding one or more significant differences in 21 tests = 65.9%. Bonferroni's adjustment indicates critical p-value = 0.002 and assuming outcomes are moderately correlated (r = 0.5) then a critical p-value = 0.01 would be required.</p

Relative abundance of miR in the conjunctiva.

<p>Abundance of all miR tested expressed relative to miR-1274B. Abundance was calculated from cycle threshold values irrespective of sample phenotype.</p

qPCR sample demographic summary including FPC grading scores (0–3) for each phenotypic group.

<p>Footnote: Age ranges between phenotypic groups were not significantly different (Wilcoxon test p>0.05). One individual in the TS group also had a follicular grade (F) of 1, and three individuals in TSI had F3.</p

DIANA mirPath pathway analysis on differentially expressed miR in each comparison group (p<0.05 and FC>3).

<p>DIANA mirPath pathway analysis on differentially expressed miR in each comparison group (p<0.05 and FC>3).</p

Factors associated with risk of Ebola virus disease transmission in 268 returnees who worked in West Africa during the 2014–2016 Ebola outbreak.

<p>Factors associated with risk of Ebola virus disease transmission in 268 returnees who worked in West Africa during the 2014–2016 Ebola outbreak.</p