Primer sequences used for real time qRT-PCR.

<p>Primer sequences used for real time qRT-PCR.</p

Generation of an ovarian cancer cell model for Ets-1 expression.

<p>2008 ovarian cancer cells were stably transfected to over-express Ets-1 in a tetracycline inducible system. Protein expression of 2008 and 2008-Ets1 cells was measured via Western blot following induction with tetracycline (n = 3).</p

Glycolytic dependence of Ets-1-expressing ovarian cancer cells.

<p>(<b>A</b>) 2008, C13*, and 2008-Ets1 cells were treated with the glycolytic inhibitor 2-DG, and cellular growth was measured following 96 hours of incubation. 2008-Ets1 and C13* cells which express Ets-1 showed significantly decreased growth following glycolytic inhibition in 2008-Ets1 compared to parental 2008 cells (n = 4). (<b>B</b>) 2008 and 2008-Ets1 cells were grown in the absence of glucose, and proliferation assays were conducted at 24 hour intervals. 2008-Ets1 ovarian cancer cells expressing high levels of Ets-1 showed a decreased ability for growth in glucose-free culture medium, suggesting an increased reliance on glycolysis for energy (n = 3).</p

Effect of Ets-1 expression on oxygen consumption.

<p>Basal oxygen consumption was determined for 2008 and 2008-Ets1 ovarian cancer cells. Ets-1 expression was associated with a significant decrease in basal oxygen consumption suggesting a decreased reliance on oxidative phosphorylation in 2008-Ets1 cells.</p

Ets-1 expression did not affect the expression of similar ETS family members.

<p>The protein expression of ETS family transcription factors (<b>A</b>) Ets-2 and (<b>B</b>) PEA3 were compared in 2008 and 2008-Ets1 ovarian cancer cells to determine the specificity of our Ets-1 expression model. Western blot analysis showed that neither of these transcription factors was affected by Ets-1 expression in 2008 cells.</p

Immunophenotyping of peripheral blood mononuclear cells (PBMCs) from young adults, community-dwelling seniors, and advanced-age, frail elderly.

<p>Mean and standard error presented. Only values with comparison-wise (Wilcoxon rank-sum test) significance at p<0.25 shown; Bolded values indicated experimental-wise (Benjamin-Hochberg FDR) significance at p<0.05. Cells/µL, cells per microliter of blood (absolute count); Rel. PBMCs, relative to PBMCs.</p