Partial gas-liquid chromatogram of the branched chain fatty acid region of the fat tail in Damara Ram lambs.

<p>Peak identification: 1) 10:0; 2) 10:0-4Me and 10:0–6Me; 3) 11:0; 4) 11:0–4Me; 5) 11:0–4,8Me; 6) 12:0; 7) 12:0–4Me; 8) 12:0–8Me; 9) 12:0–4,8Me; 10) 13:0; 11) 13:0–6Me; 12) 13:0–4Me; 13) i–14:0; 14) 13:0–4,8Me; 15) 14:0–2Me; 16) 14:0; 17) 14:0–6Me; 18) 14:0–8Me; 19) 14:0–4Me; 20) 14:0–10Me; 21) i-15:0; 22) 14:0–2,6Me; 23) a-15:0; 24) 14:1<i>cis-</i>9; 25) 15:0; 26) 15:0–8Me; 27) 15:0–4Me; 28) i-16:0; 29) 16:0–2Me; 30) 16:0; 31) 16:0–6Me; 32) 16:0–8Me; 33) 16:0–4Me; 34) 16:0–12Me; 35) i-17:0; 36) 16:1<i>cis-</i>7; 37) 16:1<i>cis-</i>9 and a-17:0; 38) 17:0; 39) 17:0–12Me; 40) i-18:0; 41) 17:1cis-9. Abbreviations: BTH, Butylated hydroxytoluene; Me, methyl; i, iso; a, anteiso.</p

Effect of feed restriction on the growth and fat tail in Damara ram lambs.

<p>Effect of feed restriction on the growth and fat tail in Damara ram lambs.</p

Effect of feed restriction in the fatty acid composition (% of total fatty acids) of the fat tail in Damara ram lambs.

<p>Abbreviations: Me, methyl; BCFA, branched-chain fatty acids; BI, biohydrogenation intermediates, include total C18 FA minus 18:0, 18:1<i>cis-</i>9, 18:1<i>cis-</i>11, 18:2n-6 and 18:3n-3.</p

Electron impact mass spectra of the: A) 16:0–4Me; B) 16:0–6Me; C) 16:0–8Me; and D) 12:0–4,8Me branched chain fatty acids in the fat tail of Damara Ram lambs.

<p>Electron impact mass spectra of the: A) 16:0–4Me; B) 16:0–6Me; C) 16:0–8Me; and D) 12:0–4,8Me branched chain fatty acids in the fat tail of Damara Ram lambs.</p

Damara fat tailed sheep.

<p>A) Damara, Dorper and Australian Merino rams at the initial stages of the feeding trial. Notice the characteristic hanging fat tail of the ram up front. B) Several aspects of the fat tail in several of the Damara rams used in this trial. C) In the first plane, brown Damara ram.</p

Partial gas-liquid chromatograms of the branched chain fatty acid region of gastrocnemius muscle samples of Damara, Merino and Dorper Ram lambs, before and after hydrogenation of the unsaturated fatty acids.

<p>Peak identification: 1) 16:0; 2) 16:0–6Me; 3) 16:0–8Me; 4) DMA-17:0; 5) 16:0–4Me; 6) 16:0–12Me; 7) i-17:0; 8) 16:1<i>cis-</i>7; 9) 16:1<i>cis-</i>9; 10) a-17:0; 11) 17:0.</p

Effect of feed restriction in total fatty acid (mg/g DM) and dimethylacetal and fatty acid composition (% of total products) of the gastrocnemius muscle in Damara ram lambs.

<p>Abbreviations: Me, methyl; DMA, dimethylacetal; BCFA, branched-chain fatty acids; BI, biohydrogenation intermediates, include total C18 FA minus 18:0, 18:1 <i>cis-</i>9, 18:1 <i>cis-</i>11, 18:2 n-6 and 18:3 n-3. ¹Co-elutes with the 20:3n-9.</p

Two-dimensional gel electrophoresis.

<p>Example of a 2D gel for a merino ram gastrocnemius muscle, showing the spots that were selected for identification. Molecular mass markers (MM, vertical scale), as well as the isoelectric point (pI, horizontal scale are also presented. Two-dimensional electrophoresis was carried out in linear 24 cm Immobiline DryStrips (pH 3–10). Gels were stained with colloidal Coomassie Blue G-250.</p

Plasma concentrations of leptin and insulin (mean (s.e.)) in three breeds of sheep fed a diet maintaining or restricting growth.

<p>Plasma concentrations of leptin and insulin (mean (s.e.)) in three breeds of sheep fed a diet maintaining or restricting growth.</p

Live weight changes (kg ± standard deviation) for Damara, Dorper and Merino breeds assigned to either a restricted or growth diet.

<p>Live weight changes (kg ± standard deviation) for Damara, Dorper and Merino breeds assigned to either a restricted or growth diet.</p