30 research outputs found
Pheromone concentration-dependent Ste50p shmoo tip localization <i>ste50Δ</i> strain, with or without Ste50-GFP on a plasmid, was treated with α-factor and imaged using epifluorescence and DIC microscopy.
No full text(A) Response of STE50 as indicated after 4h treatment with 2μM α-factor. (B) Ste50p patch localization at the shmoo tip at the indicated concentrations of α-factor for the indicated time. (C) Number of cells with localized Ste50p polarity patches at the shmoo tip at the indicated pheromone concentrations and time (n≥100 cells, N = 3). (D) Quantified polarity patches of Ste50p at the shmoo tip with respect to the cytoplasmic amount at indicated pheromone concentrations and time (see text); N = 2: 2μM 1h (mean = 0.01, SD±0.0064; n = 35), 2μM 2h (mean = 0.035, SD±0.0224; n = 75), 4μM 1h (mean = 0.028, SD±0.0163; n = 78), 4μM 2h (mean = 0.015, SD±0.0091; n = 8); ****, p0.05; one-way Anova with Tukey’s multiple comparisons. Bar represents 5μm.</p
Expression of neurotransmitters and neurotransmitter receptors in the early motor circuit.
No full text<p>A–H) In-situ detection of neurotransmitter receptor subunit mRNA (green) and <i>Isl1</i> mRNA (red) in adjacent sections reveals differential expression in medial LMC and lateral LMC motor neurons from HH St. 24–30. G) KCC2 (green) and Isl1 (red) protein detection. H–J) <i>GAD-1</i> mRNA expression in the whole spinal cord (dotted line) from HH St. 24–30. Coloured contours of spinal regions correspond to those summarizing neurotransmitter receptor expression in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093836#pone-0093836-t001" target="_blank">Table 1</a>. Scale bars represent 100 μm.</p
Variation in the synchronicity of activity with developmental age and neuron-neuron distance.
No full text<p>A) Over time, the percentage of active neurons in a preparation increases from 18.61%±7.19% to 81.01±14.02%; p<0.0001, one-way ANOVA. B) The frequency of motor neuron firing shows significant changes over time, decreasing moderately from HH St. 23 to HH St. 30. C) There is a decrease in the frequency of asynchronous cycles over time counteracted by only a small increase in the frequency of synchronous cycles (D). E) The length of asynchronous cycles decreases, whilst the length of synchronous cycles increases over time (F). G) Analysis of the distance between two given cells and the correlation between them shows that embryos at HH St. 23 and HH St. 25 are dominated by asynchronous cycles, but that older embryos have higher synchronicity over longer distances. Pixel intensity indicates the percentage of interactions at a given correlation and distance.</p
