1 research outputs found
Carboxylate-Containing Two-Photon Probe for the Simultaneous Detection of Extra- and Intracellular pH Values in Colon Cancer Tissue
Acidified extracellular
pH (pHe) is directly related to various
disorders such as tumor invasion and the resistance to drugs. In this
study, we developed two-photon-excitable emission ratiometric probes
(<b>XBH1–3</b>) for the in situ measurement of pHe. These
probes, based on benzimidazole and polar solubilizing groups, exhibited
a strong two-photon-induced fluorescence and sensitive blue-to-green
emission color changes with p<i>K</i><sub>a</sub> values
of 5.1–5.7. <b>XBH1</b>, containing a carboxylic acid,
stained the extracellular region in neutral media; it entered the
cell under acidic media, thereby allowing a precise measurement of
the extra- and intra-cellular pH values in the acidified tissue. <b>XBH2</b>, containing the sulfonate peripheral unit, facilitated
the monitoring of the pHe value only. Ratiometric two-photon microscopy
imaging revealed that <b>XBH1</b> can directly monitor the pH
values both inside and outside the cells in colon cancer tissue; there
is also the morphological aspect. This could be useful for cancer
analyses and drug development