1 research outputs found
A Self-Assembled Ratiometric Polymeric Nanoprobe for Highly Selective Fluorescence Detection of Hydrogen Peroxide
In this study, a dual-emission fluorescence
resonance energy transfer
(FRET) polymeric nanoprobe by single-wavelength excitation was developed
for sensitive and selective hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) detection. Polymeric nanoprobe was prepared by simple self-assembly
of functional lipopolymers, which were 4-carboxy-3-fluorophenylboronic
acid (FPBA)-modified DSPE-PEG (DSPE-PEG-FPBA) and 7-hydroxycoumarin
(HC)-conjugated DSPE-PEG (DSPE-PEG-HC). Subsequent binding of alizarin
red S (ARS) to FPBA endowed the nanoprobe with a new fluorescence
emission peak at around 600 nm. Because of the perfect match of the
fluorescence emission spectra of HC with the absorbance spectra of
ARS-FPBA, FRET was achieved between them. The sensing strategy for
H<sub>2</sub>O<sub>2</sub> was based on H<sub>2</sub>O<sub>2</sub>-induced deboronation reaction and boronic acid-mediated ARS fluorescence.
Interaction between phenylboronic acid and ARS was revisited herein
and it was found that electron-donating or -withdrawing group on phenylboronic
acid (PBA) has significant influence on the fluorescence property
of ARS, which enabled sensitive and selective H<sub>2</sub>O<sub>2</sub> sensing. The nanoprobe displayed two well-separated emission bands
(150 nm), providing high specificity and sensitivity for ratiometric
detection of H<sub>2</sub>O<sub>2</sub>. Further application was exploited
for the determination of glucose and the results demonstrated that
the proposed strategy showed ratiometric response capability for glucose
detection. The current method does not involve complicated organic
synthesis and opens a new avenue for the construction of multifunctional
polymeric fluorescent nanoprobe