Sequencing Analysis of Genetic Loci for Resistance for Late Leaf Spot and Rust in Peanut (Arachis hypogaea L.)

The aim of this study was to identify candidate resistance genes for late leaf spot (LLS) and rust diseases in peanut (Arachis hypogaea L.). We used a double-digest restriction-site associated DNA sequencing (ddRAD-Seq) technique based on next-generation sequencing (NGS) for genotyping analysis across the recombinant inbred lines (RILs) derived from a cross between a susceptible line, TAG 24, and a resistant line, GPBD 4. A total of 171 SNPs from the ddRAD-Seq together with 282 markers published in the previous studies were mapped on a genetic map covering 1510.1 cM. Subsequent quantitative trait locus (QTL) analysis revealed major genetic loci for LLS and rust resistance on chromosomes A02 and A03, respectively. Heterogeneous inbred family-derived near isogenic lines and the pedigree of the resistant gene donor, A. cardenasii Krapov. & W.C. Greg., including the resistant derivatives of ICGV 86855 and VG 9514 as well as GPBD 4, were employed for whole-genome resequencing analysis. The results indicated the QTL candidates for LLS and rust resistance were located in 1.4- and 2.7-Mb genome regions on A02 and A03, respectively. In these regions, four and six resistance-related genes with deleterious mutations were selected as candidates for LLS and rust resistance, respectively. These delimited genomic regions may be beneficial in breeding programs aimed at improving disease resistance and enhancing peanut productivity

Stability analysis for yield and yield components over seasons in cowpea [Vigna unguiculata L. (Walp.)]

Twenty diverse genotypes including one local check (C-152) of cowpea were evaluated over three seasons to study the stability parameters viz.,regression coefficient (bi) and mean square deviations (s2di) from linear regression along with per se performance for five yield related traits.Variances due to genotype, environment, genotype x environment, environment + (genotype x environment), environment (linear) weresignificant for pods per plant and seed yield per plant. Based on the stability analysis, the genotype IL3 was found stable across the seasons fortest weight. Genotypes such as M17, Goa local and Bailhongal local were stable and superior across all the environments for seed yield

Estimates of Variability, Heritability, Genetic Advance for Yield and its Quality Traits in Onion (Allium cepa L.) Genotypes

The present investigation to estimate genetic variability was carried out with forty genotypes of onion which were collected from different locations of Karnataka and were evaluated for growth yield and quality traits. Among all the genotypes studied, Gadag Local exhibited highest plant height at 30 DAT (34.20 cm), 50 DAT (43.46) and 90 DAT (51.76 cm), highest number of leaves were produced by Nasik Red at 50 DAT (9.40 cm) and Savalagi Local at 90 DAT (13.53 cm), Sathara Local exhibited highest equatorial diameter (4.54 cm). highest total soluble solids (15.03%) was recorded by Gadag Local.  Kalasakoppa Local produced highest dry matter (20.16%) and      B-780 took less number of days to maturity (100days), Bilagi Local had highest polar diameter (4.93 cm). The high GCV and PCV was observed for ten bulb weight and leaf diameter respectively. High estimates of heritability coupled with high genetic advance as percent of mean was observed for ten bulb weight and Total yield(q/ha)  Hence, for future onion improvement, these well performing genotypes can be subjected to selection and also used as parents in hybridization programme to obtain varieties/hybrids with desirable quality and higher yield

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Not AvailableThe identification of marker tightly linked to male sterility will greatly facilitate for marker assisted selection (MAS) breeding through accurate selection of parental lines in hybrid production. In the present study, to assess the efficiency of previously reported SCAR 4 marker for marker assisted selection was validated by screening the marker in a total of 226 F2 mapping population derived from a cross between male sterile (IIHR10521AB) and a male fertile pure line (IIHRMY7) maintained at IIHR along with bulk segregant analysis. The results showed that the marker segregated in the F2 population showing that it is linked to sterility locus. The marker was also validated by screening 12 different apetalloid male sterile lines maintained at IIHR, the results of amplification gave clear and similar band size amplicons present in parents in all the apetalloid sterile lines confirming that it is linked to male sterility and hence this study is significantly useful and can offer a powerful tool for the efficient selection in MAS breeding programmes in marigold.Not Availabl