Comparison of anti-<i>Leishmania</i> antibody responses in rKLO8- and rK39 ELISA using patient and control sera from three endemic regions.

<p>Protein concentrations of 5ng/100μl rKLO8 or rK39 were tested with sera of patients and controls from Sudan (A), India (B) and France (C). Sera were tested at dilutions of 1:800. A cut off value of 0.12 was established as means + 3 SD of 30 healthy controls. VL, visceral leishmaniasis; VLS, VL suspects; HC, healthy control; MA, malaria; TP, toxoplasmosis; VL/HIV, VL and HIV, ASC, asymptomatic cases. The black horizontal lines represent cut off values. OD values of 0.6 (5 fold of cut off) and 2.15 are shown in dotted lines.</p

Reactivity of VL sera from different endemic regions in rKLO8- and rK39 ELISA.

<p>OD values of VL sera from Sudan (n = 50), India (n = 26) and France with negative (HIV-, n = 26) or positive (HIV+, n = 11) HIV were tested in rKLO8- or rK39 ELISA at dilutions of 1:800. Means ODs of VL sera from the different regions were compared. Dots represent values for individual sera and horizontal line represents cut-off values (0.12). Significance was determined by unpaired Student's <i>t</i> test at 95% confidence intervals and P-values < 0.05 indicate statistically significant differences; ns indicates no significant differences.</p