1 research outputs found
A comprehensive study on bisphenol A degradation by newly isolated strains Acinetobacter sp. K1MN and Pseudomonas sp. BG12
Bisphenol A (BPA) is an endocrine disrupting
chemical. Its extensive use has led to the wide
occurrence of BPA in various environmental ecosystems,
at levels that may cause negative effects to the
ecosystem and public health. Although there are many
bacteria able to BPA utilization, only a few of them
have a strong capacity for its biodegradation. Therefore,
it is important to search for new bacteria strains,
investigate their BPA biodegradation ability and
potential effect of pH and other organic compounds
on the process. These tasks have become the object of
the present study. The results of our research show that
for the newly isolated strains Acinetobacter sp. K1MN
and Pseudomonas sp. BG12 after 15 days, with an
initial BPA concentration of 100 mg L- 1, the highest
BPA removal was achieved at pH 8, while sodium
glutamate as a biostimulant best accelerated BPA
degradation. Kinetic data for BPA biodegradation by
both strains best fitted the Monod model. The specific
degradation rate and the half saturation constant were
estimated respectively as 8.75 mg L- 1 day- 1 and
111.27 mg L- 1 for Acinetobacter sp. K1MN, and
8.6 mg L- 1 day- 1 and 135.79 mg L- 1 for Pseudomonas
sp. BG12. The half-maximal effective concentration
(EC50) of BPA for Acinetobacter sp. K1MN
was 120 mg L- 1 and for Pseudomonas sp. BG12 it
was 123 mg L- 1. The toxicity bioassay (Microtox
test) showed that elimination of BPA by both strains is
accompanied by reduction of its toxic effect. The
ability of tested strains to degrade BPA combined with
their high resistance to this xenobiotic indicates that
Acinetobacter sp. K1MN and Pseudomonas sp. BG12
are potential tools for BPA removal during wastewater
treatment plant