Blood routine and biochemistry between the transgenic offspring and the non-transgenic control pigs.

<p>Blood routine and biochemistry between the transgenic offspring and the non-transgenic control pigs.</p

Cardiac structure and function between the transgenic offspring and the non-transgenic control pigs.

<p>Cardiac structure and function between the transgenic offspring and the non-transgenic control pigs.</p

Generation of red fluorescent protein (DsRed) transgenic pigs by pronuclear micro-injection.

<p>Generation of red fluorescent protein (DsRed) transgenic pigs by pronuclear micro-injection.</p

Fluorescence microscopy of the paraffin-embedded tissue sections of DsRed transgenic pigs revealed evident red fluorescence.

<p>Fluorescence microscopy of the paraffin-embedded tissue sections of DsRed transgenic pigs revealed evident red fluorescence.</p

Bright-field images of tissues from the transgenic pig offspring (left) depict a distinct red fluorescence in comparison with the nontransgenic control (right).

<p>Bright-field images of tissues from the transgenic pig offspring (left) depict a distinct red fluorescence in comparison with the nontransgenic control (right).</p

The DsRed-Monomer transgenic construct pCX-DsRed-Monomer.

<p>Arrows indicate the positions of the PCR DsRed primers. XbaI and DraI were restriction enzyme digestion sites. The thick black line indicates the position of the Southern blot probe.</p

Transgenic piglets and (white arrow) and nontransgenic controls (white triangle) were differentiated according to the presence of external red fluorescence.

<p>Transgenic piglets and (white arrow) and nontransgenic controls (white triangle) were differentiated according to the presence of external red fluorescence.</p

Generation and Characterization of a Transgenic Pig Carrying a DsRed-Monomer Reporter Gene - Figure 8

<p>(a) Cytometric analyses of CD4a, CD31, CD44, and CD90 conjugated with FITC on DsRed pig amniotic fluid progenitor/stem cells (pAFPCs). pAFPCs were negative for the surface antigens CD4a and CD31, and positive for CD44 and CD90. (b) Mesoderm trilineage differentiation potential of DsRed pAFPCs. DsRed pAFPCs exhibited spindle-shaped morphology (A1) and expressed red fluorescence under fluorescent microscopy (A2). Lipid droplets were observed in the DsRed pAFPCs adipogenic differentiation culture by using Oil-Red O staining (B1). Calcium accumulation was observed in the osteogenic differentiation culture by using Alizarin red staining (C1). Collagen was detected in the chondrogenic differentiation culture by using toluidine blue staining (D1). Under fluorescent microscopy, red fluorescence was still evident after osteogenetic (B2), adipogenetic (C2), and chondrogenetic (D2) differentiation.</p

Transgenic founder pigs carrying the DsRed gene were detected by (a) PCR and (b) Southern blot analysis.

<p>The presence of transgenes in DsRed2 pigs were confirmed by using a DsRed primer, which produced a 780-bp PCR product. The genomic DNA of these 2 transgenic pigs (Nos. 1 and 3) were digested using XbaI and DraI. The digested DNA was subsequently hybridized using a 1.1-kb probe.</p

Whole-body CT of the normal and transgenic pigs.

<p>Neither structural differences nor abnormal lesions were observed in the DsRed pig.</p