La rete della sanità

Il contributo analizza e discute le dinamiche della rete sanitaria italian

Inducibility of AhR-regulated CYP genes by beta-naphtoflavone in the liver, lung, kidney and heart of the pig

the presence and inducibility of CYP enzymes belonging to the family 1 (CYP 1A1, 1A2 and 1B!) and AhR have been studied in liver, lung, kidney and heart of control and beta-naphtoflavone (betaNF)-treated pigs. Segments of so far undescribed genes for porcine CYP 1A1, 1A2, 1B1 and Ahr were identified by RT_PCR and their sequences found to be higly homologous to those of the corresponding human genes. Our results demonstrated a differential expression and regulation of the AhR-mediated CYP genes in liver, lung, kidney and heart of the pig-naphtoflavone

Expression and activity of CYP 1A1 in different brain regions of control and beta-naphthoflavone-treated pigs

Expression and activity of CYP 1A1 in different brain regions of control and beta-naphthoflavone-treated pigs Gervasi, P.G.1, Chirulli, V.1, Zaghini, A.2, Vaccaro, E.3, Fiorio, R.3, Longo, V.3, Marvasi, L.2 1 Ins. Clinical Physiology, Metabolism, Pisa, Italy; 2 Bologna University, Patologia Veterinaria, Bologna, Italy; 3 Ins. Clinical Physiology, Metabolism, PISA, Italy The expression of the cytochrome P450 1A1 (CYP 1A1) and its related activities along with the Ah Receptor were investigated in liver and brain regions from control pigs and pigs treated with beta-naphthoflavone (30 mg/kg i.p. for 4 days). By RT-PCR analysis, using specific primers, it was demonstrated that mRNA of CYP 1A1 and AhR were expressed, although at different extent, in the liver, cortex, midbrain and cerebellum. However, the beta-naphthoflavone treatment had no apparent effect on expression pattern of this isoform, unlike liver, in any brain region. Then, some enzymatic activities (ethoxyresorufin O-deethylase and methoxyresorufin O-demethylase), known markers for the CYP 1A1/2, were studied in microsomes of liver and microsomes and mitochondria of cortex, midbrain and cerebellum from control and beta-naphthoflavone treated pigs. The enzymatic analysis revealed a presence of these activities about 3-4 times higher in mitochondria than in microsomes from all the brain regions examined. However, in contrast to the behaviour of the liver, beta-naphthoflavone treatment did not induce the cerebral activities. Despite the presence of AhR, the CYP1A1 in the pig brain was resistant to beta-naphthoflavone induction suggesting that there is a different regulation mechanism of this enzyme between the brain and liver

The Administrative Board of Review of the European Central Bank: Experience After 2 Years

This article examines the administrative remedy provided by the Administrative Board of Review (ABoR) of the European Central Bank (ECB), as part of the broader issue of the right of defence of natural and legal persons vis-à-vis ECB supervisory decisions within the Single Supervisory Mechanism (SSM). After presenting an overview of the review panels established in the financial sector in the EU, the article describes the experience with the ABoR by analysing its composition, its mandate and scope of review, the main procedural aspects and the relationship with judicial proceedings before the European Court of Justice. Particular attention is given to the substantial issues dealt with by the ABoR in its Opinions and to some of the major challenges faced in the first 2 years of practice. Among them is the assessment of the correct application of national laws implementing EU legislation by the ECB. The paper identifies two aspects giving particular cause for concern and requiring legislative reforms: (1) the assessment of the suitability of the members of management bodies (fit and proper assessment) and (2) the inclusion of bank holding companies within the scope of banking supervision

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