80 research outputs found
Effect of Depth of Planting and Storage of Wet and Dry Roots on the Germination of Four Varieties of Forage-type Bermudagrass
Agronomy (Field Crops
The Neisseria Lipooligosaccharide-Specific Alpha-2,3-Sialyltransferase is a Surface-Exposed Outer Membrane Protein
Neisseria gonorrhoeae and Neisseria meningitidis express an similar to43-kDa alpha-2,3-sialyltransferase (Lst) that sialylates the surface lipooligosaccharide (LOS) by using exogenous (in all N. gonorrhoeae strains and some N. meningitidis serogroups) or endogenous (in other N. meningitidis serogroups) sources of 5\u27-cytidinemonophospho-N-acetylneuraminic acid (CMP-NANA). Sialylation of LOS can protect N. gonorrhoeae and N. meningitidis from complement-mediated serum killing and from phagocytic killing by neutrophils. The precise subcellular location of Lst has not been determined. We confirm and extend previous studies by demonstrating that Lst is located in the outer membrane and is surface exposed in both N. gonorrhoeae and N. meningitidis. Western immunoblot analysis of subcellular fractions of N. gonorrhoeae strain F62 and N. meningitidis strain MC58not subset of3 (an acapsulate serogroup B strain) performed with rabbit antiserum raised against recombinant Lst revealed;an similar to43-kDa protein exclusively in outer membrane preparations of both pathogens. Inner membrane, periplasmic, cytoplasmic, and culture supernatant fractions were devoid of Lst, as determined by Western blot analysis. Consistent with this finding, outer membrane fractions of N, gonorrhoeae were significantly enriched for sialyltransferase enzymatic activity. A trace of enzymatic activity was detected in inner membrane fractions, which may have represented Lst in transit to the outer membrane or may have represented inner membrane contamination of outer membrane preparations. Subcellular preparations of an isogenic lst insertion knockout mutant of N. gonorrhoeae F62 (strain ST01) expressed neither a 43-kDa immunoreactive protein nor sialyltransferase activity. Anti-Lst rabbit antiserum bound to whole cells of N. meningitidis MC58not subset of3 and wild-type N. gonorrhoeae F62 but not to the Lst mutant ST01, indicating the surface exposure of the enzyme. Although the anti-Lst antiserum avidly bound enzymatically active, recombinant Lst, it inhibited Lst (sialyltransferase) activity by only about 50% at the highest concentration of antibody, used. On the contrary, anti-Lst antiserum did not inhibit sialylation of whole N. gonorrhoeae cells in the presence of exogenous CMP-NANA, suggesting that the antibody did not bind to or could not access the enzyme active site on the surface of viable Neisseria cells. Taken together, these results indicate that Lst is an outer membrane, surface-exposed glycosyltransferase. To our knowledge, this is the first demonstration of the localization of a bacterial glycosyltransferase to the outer membrane of gram-negative bacteria
Versatile silicon-waveguide supercontinuum for coherent mid-infrared spectroscopy
Infrared spectroscopy is a powerful tool for basic and applied science. The
molecular spectral fingerprints in the 3 um to 20 um region provide a means to
uniquely identify molecular structure for fundamental spectroscopy, atmospheric
chemistry, trace and hazardous gas detection, and biological microscopy. Driven
by such applications, the development of low-noise, coherent laser sources with
broad, tunable coverage is a topic of great interest. Laser frequency combs
possess a unique combination of precisely defined spectral lines and broad
bandwidth that can enable the above-mentioned applications. Here, we leverage
robust fabrication and geometrical dispersion engineering of silicon
nanophotonic waveguides for coherent frequency comb generation spanning 70 THz
in the mid-infrared (2.5 um to 6.2 um). Precise waveguide fabrication provides
significant spectral broadening and engineered spectra targeted at specific
mid-infrared bands. We use this coherent light source for dual-comb
spectroscopy at 5 um.Comment: 26 pages, 5 figure
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