Effect of silencing of the apoplastic invertase gene on photosynthesis in tomato

© 2015 Pleiades Publishing, Ltd. Photosynthesis was examined in wild-type tomato plants (Lycopersicon esculentum L., cv. Money-maker) and in the transformants where gene expression of the leaf apoplastic invertase was suppressed by RNA interference (Lin8-RNAi). The influence of genetic transformation on photosynthesis depended on the demand for assimilates by sink organs. Using growth pots with low amount of soil, we found that at the initial growth stage when growth processes were particularly active, photosynthesis in Lin8-RNAi plants was higher than in the wild-type plants. As the reserves of mineral nutrients were gradually exhausted, photosynthesis decreased in both plant groups, but the decrease was more extensive in Lin8-RNAi plant form. Analysis of the distribution of 14C among the photosynthates produced after 3-min period of 14CO2 assimilation revealed the decreased incorporation of 14C into hexoses in Lin8-RNAi plants and the increased incorporation of 14C into aspartate and products of the glycolate pathway. Supplementing the soil with nitrate nitrogen as a fertilizer enhanced the non-carbohydrate trend of photosynthesis, but this trend was less pronounced in the transformed plants. Simultaneous measurements of CO2-exchange and H2O release revealed an insignificant increase in Lin8-RNAi plants of photosynthetic activity, transpiration, and intraleaf CO2 concentration. However, in 30–50 min after lowering the photon flux density from 1556 to 771 μmol/(m2 s) photosynthesis was reduced in both genotypes, whereas transpiration was diminished in wild-type plants and increased in the Lin8-RNAi form. It is concluded that the apoplastic invertase regulates photosynthesis through changes in osmolarity of the apoplastic fluid that controls the opening of stomata

Television pictures of Phobos: first results

In February-March 1989, 37 television images of the Martian satellite Phobos were obtained by the Phobos 2 spacecraft from distances of 200-1100 km. These images provide an important supplement to the TV data from the American Mariner 9 and Viking spacecraft in coverage of t4e surface of Phobos and in resolution in certain regions, in spectral range, and in range of phase angles. They make it possible to refine the figure and topographic and geological maps of the surface of Phobos, its spectral and angular reflective characteristics, the surface composition and texture, and characteristics of the orbital and librational motion

Effect of silencing of the apoplastic invertase gene on photosynthesis in tomato

© 2015 Pleiades Publishing, Ltd. Photosynthesis was examined in wild-type tomato plants (Lycopersicon esculentum L., cv. Money-maker) and in the transformants where gene expression of the leaf apoplastic invertase was suppressed by RNA interference (Lin8-RNAi). The influence of genetic transformation on photosynthesis depended on the demand for assimilates by sink organs. Using growth pots with low amount of soil, we found that at the initial growth stage when growth processes were particularly active, photosynthesis in Lin8-RNAi plants was higher than in the wild-type plants. As the reserves of mineral nutrients were gradually exhausted, photosynthesis decreased in both plant groups, but the decrease was more extensive in Lin8-RNAi plant form. Analysis of the distribution of 14C among the photosynthates produced after 3-min period of 14CO2 assimilation revealed the decreased incorporation of 14C into hexoses in Lin8-RNAi plants and the increased incorporation of 14C into aspartate and products of the glycolate pathway. Supplementing the soil with nitrate nitrogen as a fertilizer enhanced the non-carbohydrate trend of photosynthesis, but this trend was less pronounced in the transformed plants. Simultaneous measurements of CO2-exchange and H2O release revealed an insignificant increase in Lin8-RNAi plants of photosynthetic activity, transpiration, and intraleaf CO2 concentration. However, in 30–50 min after lowering the photon flux density from 1556 to 771 μmol/(m2 s) photosynthesis was reduced in both genotypes, whereas transpiration was diminished in wild-type plants and increased in the Lin8-RNAi form. It is concluded that the apoplastic invertase regulates photosynthesis through changes in osmolarity of the apoplastic fluid that controls the opening of stomata

Effect of silencing of the apoplastic invertase gene on photosynthesis in tomato

© 2015 Pleiades Publishing, Ltd. Photosynthesis was examined in wild-type tomato plants (Lycopersicon esculentum L., cv. Money-maker) and in the transformants where gene expression of the leaf apoplastic invertase was suppressed by RNA interference (Lin8-RNAi). The influence of genetic transformation on photosynthesis depended on the demand for assimilates by sink organs. Using growth pots with low amount of soil, we found that at the initial growth stage when growth processes were particularly active, photosynthesis in Lin8-RNAi plants was higher than in the wild-type plants. As the reserves of mineral nutrients were gradually exhausted, photosynthesis decreased in both plant groups, but the decrease was more extensive in Lin8-RNAi plant form. Analysis of the distribution of 14C among the photosynthates produced after 3-min period of 14CO2 assimilation revealed the decreased incorporation of 14C into hexoses in Lin8-RNAi plants and the increased incorporation of 14C into aspartate and products of the glycolate pathway. Supplementing the soil with nitrate nitrogen as a fertilizer enhanced the non-carbohydrate trend of photosynthesis, but this trend was less pronounced in the transformed plants. Simultaneous measurements of CO2-exchange and H2O release revealed an insignificant increase in Lin8-RNAi plants of photosynthetic activity, transpiration, and intraleaf CO2 concentration. However, in 30–50 min after lowering the photon flux density from 1556 to 771 μmol/(m2 s) photosynthesis was reduced in both genotypes, whereas transpiration was diminished in wild-type plants and increased in the Lin8-RNAi form. It is concluded that the apoplastic invertase regulates photosynthesis through changes in osmolarity of the apoplastic fluid that controls the opening of stomata

Effect of silencing of the apoplastic invertase gene on photosynthesis in tomato

© 2015 Pleiades Publishing, Ltd. Photosynthesis was examined in wild-type tomato plants (Lycopersicon esculentum L., cv. Money-maker) and in the transformants where gene expression of the leaf apoplastic invertase was suppressed by RNA interference (Lin8-RNAi). The influence of genetic transformation on photosynthesis depended on the demand for assimilates by sink organs. Using growth pots with low amount of soil, we found that at the initial growth stage when growth processes were particularly active, photosynthesis in Lin8-RNAi plants was higher than in the wild-type plants. As the reserves of mineral nutrients were gradually exhausted, photosynthesis decreased in both plant groups, but the decrease was more extensive in Lin8-RNAi plant form. Analysis of the distribution of 14C among the photosynthates produced after 3-min period of 14CO2 assimilation revealed the decreased incorporation of 14C into hexoses in Lin8-RNAi plants and the increased incorporation of 14C into aspartate and products of the glycolate pathway. Supplementing the soil with nitrate nitrogen as a fertilizer enhanced the non-carbohydrate trend of photosynthesis, but this trend was less pronounced in the transformed plants. Simultaneous measurements of CO2-exchange and H2O release revealed an insignificant increase in Lin8-RNAi plants of photosynthetic activity, transpiration, and intraleaf CO2 concentration. However, in 30–50 min after lowering the photon flux density from 1556 to 771 μmol/(m2 s) photosynthesis was reduced in both genotypes, whereas transpiration was diminished in wild-type plants and increased in the Lin8-RNAi form. It is concluded that the apoplastic invertase regulates photosynthesis through changes in osmolarity of the apoplastic fluid that controls the opening of stomata

Isentropic compression of condensed argon by ultra-high magnetic field pressure. 2: Results of the experiment

The paper presents the main results of the second experiment on isentropic compression of condensed argon by ultra-high magnetic field pressure, using magnetocumulative generator MC-1. The work has been done within the scope of VNIIEF-LANL 0002P0004-95 contract, task-020. During the experiment the initial magnetic field of MC-1 generator (140 kOe) was measured, the state of electroconductive properties of a compressed sample was registered, a pulsed X-ray image of a compression device was exposed, and all events of the experiment were synchronized at the timescale of compression process. During the joint Russian-American study of the argon isentropically compressed by megabar pressure it was recorded for the first time the argon`s conductivity state under compression of {ge} 4 times, according to the estimates based on the experimental X-ray image