Chromatin dynamics resolved with force spectroscopy

In eukaryotic cells, genomic DNA is organized in chromatin fibers composed of nucleosomes as structural units. A nucleosome contains 1.7 turns of DNA wrapped around a histone octamer and is connected to the adjacent nucleosomes with linker DNA. The folding of chromatin fibers effectively increases the compaction of genomic DNA, but it remains accessible for enzymatic reactions. This apparent paradox motivates a detailed study of the dynamics of chromatin. A structural model at the molecular level will shed light on how cells regulate the compaction and dynamics of genomic DNA. This thesis presents the results of an experimental study on the dynamics of chromatin higher-order folding. Using magnetic tweezers, we observed force-dependent structural changes within chromatin fibers at the single nucleosome level.LEI Universiteit LeidenBiological and Soft Matter Physic

Expression, crystallization and preliminary X-ray diffraction studies of N-carbamyl-D-amino-acid amidohydrolase from Agrobacterium radiobacter

The Agrobacterium radiobacter CCRC 14924 N-carbamyl-D-amino-acid amidohydrolase, the enzyme used for production of D-amino acids, was overexpressed in Escherichia coli JM109. The expressed protein was crystallized by vapour diffusion using lithium sulfate as precipitant. It crystallizes in space group P2(1) with unit-cell parameters cr = 69.8, b = 67.9 and c = 137.8 Angstrom and beta = 96.4 degrees. There are four molecules per asymmetric unit. Crystals diffract to 2.8 Angstrom resolution using a rotating-anode source at cryogenic (113 K) temperatures

Crystal structure and site-directed mutagenesis studies of N-carbamoyl-D-amino-acid amidohydrolase from Agrobacterium radiobacter reveals a homotetramer and insight into a catalytic cleft

The N-carbamoyl-D-amino-acid amidohydrolase (D-NCAase) is used on an industrial scale for the production of D-amino acids. The crystal structure of D-NCAase was solved by multiple isomorphous replacement with anomalous scattering using xenon and gold derivatives, and refined to 1.95 Angstrom resolution, to an X-factor of 18.6 %. The crystal structure shows a four-layer alpha/beta fold with two six-stranded beta sheets packed on either side by two ct helices. One exterior layer faces the solvent, whereas the other one is buried and involved in the tight intersubunit contacts. A long C-terminal fragment extends from a monomer to a site near a dyad axis, and associates with another monomer to form a small and hydrophobic cavity, where a xenon atom can bind. Site-directed mutagenesis of His129, His144 and His215 revealed strict geometric requirements of these conserved residues to maintain a stable conformation of a putative catalytic cleft. A region located within this cleft involving Cys172, Glu47, and Lys127 is proposed for D-NCAase catalysis and is similar to the Cys-Asp-Lys site of N-carbamoylsarcosine amidohydrolase. The homologous active-site framework of these enzymes with distinct structures suggests convergent evolution of a common catalytic mechanism. (C) 2001 Academic Press

Poling effects of dielectric and optical properties on Pb(Zn1/3Nb2/3)1-x TixO3 crystal

Journal of Rare Earths24SUPPL.166-168JREA

An application of risk-informed evaluation on MOVs and AOVs for Taiwan BWR-type nuclear power plants

Implementing a risk-informed inservice testing (RI-IST) program provides a good aspect to the nuclear power plant licensee as an alternating program in the current ASME Section XI and 10 CFR 50.55a relevant testing programs. RI-IST concentrates testing resources on highly significant components, reduces excess testing burden, increases plant's availability, decreases dose rate on the plant's staff and also reduces cost on plant's operation and maintenance under nuclear safety expectations. Furthermore, RI-IST also gives a feature on prospective licensing change basis to a nuclear power plant's licensee. This study will focus on safety-related and PRA-molded motor-operated valves (MOVs) and air-operated valves (AOVs) under the inservice testing program in boiling water reactor (BWR)-type nuclear power plant. As MOVs and AOVs have crucial safety functions throughout the nuclear power plant's safety systems, the steady operation and performance of MOVs and AOVs will definitely ensure that the nuclear power plant operates under safety expectations; therefore, this is the key reason to implement risk-informed evaluation for MOVs and AOVs in this study and being able to provide the safety significance classification for MOVs and AOVs under the current IST program to the plant's management. As a pilot study of RI-IST, the methodology of qualitative assessment will incorporate with probabilistic risk assessment (PRA) analyzing MOVs' and AOVs' safety significance within the current PRA model. The evaluating result will then classify its safety significance into a high-safety significant component (HSSC) and a low-safety significant component (LSSC) for MOVs and AOVs based on relevant regulatory criteria. With this initiating achievement, it can provide a cornerstone for further studies on the other types of valves and pumps in RI-IST program and also provide a valuable reference as proposing license change to the licensee. (C) 2007 Elsevier Ltd. All rights reserved

Risk evaluation for motor operated valves in an Inservice Testing Program at a PWR nuclear power plant in Taiwan

Safety related valves such as Motor Operated Valves (MOV), Air Operated Valves (AOV) or Check Valves (CV) play an important role in nuclear power plant. Functioning of these valves mainly aim at emergency reactivity control, post-accident residue heat removal, post-accident radioactivity removal and containment isolation when a design basis accident occurred. In order to maintain these valves under operable conditions, an Inservice Testing Program (IST) is defined for routine testing tasks based on the ASME Boiler & Pressure Vessel Code section XI code requirements. Risk based Inservice Testing Programs have been studied and developed extensively in the nuclear energy industry since the 1990s. Risk Based evaluations of IST can bring positive advantages to the licensee such as identifying the vulnerability of the system, reducing unnecessary testing burden, concentrating testing resources on the critical pass oriented valves and saving plant's personnel dose exposure. This risk evaluation is incorporated with quantitative and qualitative analyses to the Motor Operated Valves under current Inservice Testing Program for PWR nuclear power plant in Taiwan. With the outcome of the risk classifications for the safety related MOVs through numerical or deterministic analyses, a risk based testing frequency relief is suggested to demonstrate the benefits received from the risk based Inservice Testing Program. The goal made of this study, it could be as a reference and cornerstone for the licensee to perform overall scope Risk-Informed Inservice Testing Program (RI-IST) evaluation by referring relevant methodologies established in this study. (C) 2011 Elsevier Ltd. All rights reserved

A poly-Si thin-film transistor with the in situ vacuum gaps under the T-shaped-gated electrode

A T-shaped-gated (T-gate) poly-Si thin-film transistor (TFT) with symmetric vacuum gaps has been proposed and fabricated simply with a selective-etching technique and an in situ vacuum encapsulation. The proposed TFT has demonstrated a higher maximum on-off current ratio and superior reliability compared to the conventional TFTs. This is attributed to the resulting offset region and vacuum gap to reduce the off-state leakage current and improve the hot-carrier reliability, while the extra subgate serves to induce an inversion layer at the offset region to maintain the on current during the on state. Therefore, such a T-gate poly-Si TFT is very suitable for manufacturing and applications in active-matrix flat panel electronics. (c) 2006 The Electrochemical Society