Per una nuova edizione delle "Annotationi et Discorsi sopra alcuni luoghi del Decameron" (1574): il Proemio

Ricerca sui depositi borghiniani presso la Biblioteca Mediceo Laurenziana e la Biblioteca Nazionale Centrale di Firenze

Preliminari per una edizione degli scritti danteschi di Vincenzio Borghini

Un tentativo di porre in ordine il materiale inedito, presente nelle biblioteche fiorentine, che concerne gli studi danteschi di Vincenzio Borghin

IL TEMA CONSOLATORIO NELL'EPISTOLARIO TRA FRANCESCO NELLI E PETRARCA

L'indagine affronta la corrispondenza epistolare di Francesco Nelli, dal punto di vista consolatorio

Vincenzio Borghini. Filologia e invenzione nella Firenze di Cosimo I (catalogo)

Mostra di manoscritti borghiniani organizzata dalla Biblioteca Nazionale di Firenze e stesura del catalogo

Urinary prostasin: a candidate marker of ENaC activation in humans:study of preliminary feasibility

Prostasin is a serine peptidase hypothesized to regulate epithelial sodium channel (ENaC) activity in animals or on in vitro cultured cells. We investigated whether urinary prostasin may be a candidate marker of ENaC activation in humans. We studied 10 healthy volunteers and 8 hypertensive patients with raised aldosterone-to-renin ratio before and after spironolactone or saline/Florinef suppression test, respectively. Four healthy subjects were also studied before and after saline. Urinary prostasin was evaluated by SDS-PAGE, 2D maps, and Western blotting. Every sample of normotensive individuals was compared with the corresponding sample of urine collected after spironolactone or saline; every sample of hypertensive patients was compared with the corresponding sample of urine collected after saline or Florinef. Prostasin was detectable in all subjects regardless of gender, dietary sodium intake, and spironolactone treatment. Spironolactone (100 mg) increased urinary Na/K ratio and decreased urinary prostasin in normotensives in whom the renin/aldosterone axis was activated by a low Na intake, but it was ineffective in individuals with high Na intake. Saline infusion also reduced prostasin in normotensive subjects. In contrast, prostasin paradoxically increased in urine of patients affected by primary aldosteronism after volume expansion. By 2D immunoblotting, several protein isoforms were observed, some of them being overexpressed after inhibition tests in patients with primary aldosteronism. In addition to a \u201cbasal\u201d aliquot of prostasin, constitutively released in human urine regardless of sodium balance and aldosterone activation, there exists a second \u201caldosterone-responsive\u201d aliquot modulated by Na intake and potentially suitable as candidate marker of ENaC activation