Ultrasonication-Assisted Spray Ionization Mass Spectrometry for the Analysis of Biomolecules in Solution

In this paper, we describe a novel technique—ultrasonication-assisted spray ionization (UASI)—for the generation of singly charged and multiply charged gas-phase ions of biomolecules (e.g., amino acids, peptides, and proteins) from solution; this method employs a low-frequency ultrasonicator (ca. 40 kHz) in place of the high electric field required for electrospray ionization. When a capillary inlet is immersed into a sample solution within a vial subjected to ultrasonication, the solution is continually directed to the capillary outlet as a result of ultrasonication-assisted capillary action; an ultrasonic spray of the sample solution is emitted at the outlet of the tapered capillary, leading to the ready generation of gas-phase ions. Using an ion trap mass spectrometer, we found that singly charged amino acid and multiply charged peptides/proteins ions were generated through this single-step operation, which is both straightforward and extremely simple to perform. The setup is uncomplicated: only a low-frequency ultrasonicator and a tapered capillary are required to perform UASI. The mass spectra of the multiply charged peptides and proteins obtained from sample solutions subjected to UASI resemble those observed in ESI mass spectra

Size, Temperature, and Strain-Rate Dependence on Tensile Mechanical Behaviors of Ni 3

This study focuses on exploring the mechanical properties and nonlinear stress-strain behaviors of monoclinic Ni3Sn4 single crystals under uniaxial tensile test and also their size, temperature, and strain-rate dependence through constant temperature molecular dynamics (MD) simulation using Berendsen thermostat. The deformation evolution of the Ni3Sn4 atomic nanostructure during the tensile test is observed. In addition, the tensile yield strains of various Ni3Sn4 single crystals at different strain rates and temperatures are characterized through unloading process. At last, by way of linear regression analysis, the corresponding normal elastic stiffness constants are approximated and then compared with the literature theoretical data. The radial distribution function analysis shows that Ni3Sn4 single crystal in a one-dimensional nanowire configuration would become a highly disordered structure after thermal equilibration, thereby possessing amorphous-like mechanical behaviors and properties. The initial elastic deformation of Ni3Sn4 single crystal is governed by the reconfiguration of surface atoms, and its deformation evolution after further uniaxial tensile straining is characterized by Ni=Sn bond straightening, bond breakage, inner atomic distortion, cross-section shrinking, and rupture. The calculated normal elastic constants of Ni3Sn4 single crystal are found to be consistent with the literature theoretical data

Surface-Assisted Laser Desorption/Ionization Mass Spectrometry on Titania Nanotube Arrays

Titania nanotube arrays (NTA) generated from anodizing processes are tested as the substrate for surface-assisted laser desorption/ionization mass spectrometry (SALDI MS). The background generated from titania NTA is very low, making the approach suitable for the analysis of small molecules. The upper detectable mass is ∼29 kDa. Homogeneous sample deposition leads to good shot-to-shot reproducibility and suitability for quantitative analysis. Additionally, phosphopeptides can be selectively trapped on the titania NTA substrate, as illustrated by simply depositing a tryptic digest of β-casein followed by titania NTA SALDI MS analysis. The detection limit for small organics and peptides is in low fmol

Acute Fatty Liver of Pregnancy in a Taiwanese Tertiary Care Center: A Retrospective Review

SummaryObjectiveTo evaluate the demographics, clinical presentations, laboratory findings, and maternal and fetal outcomes in patients with acute fatty liver of pregnancy.Materials and MethodsA retrospective review was conducted of the records of pregnant patients with a diagnosis of acute fatty liver in a tertiary medical center over a 22-year period.ResultsEighteen patients with acute fatty liver of pregnancy were recruited, all of whom developed the disease in the third trimester. Eleven women (61%) were primigravid and four (22%) had twin pregnancies; six (33%) were diagnosed antepartum, and the other 12 (67%) were diagnosed postpartum. There were two maternal deaths (11%) and four fetal deaths (18%). The most common complications apart from severe liver dysfunction were acute renal failure (83%), hypoglycemia (61%), and disseminated intravascular coagulation (61%).ConclusionWomen who become acutely ill during the third trimester of pregnancy should undergo tests for acute fatty liver of pregnancy, including laboratory tests for assessing liver function and coagulation profile

Projected Economic and Financial Benefits of the 2017 Summer Universiade Games

In Western society, sport economic impact studies are often utilized to showcase the financial benefits for hosting mega sport event or to justify the spending for entering bids or covering infrastructure construction costs. This study projected economic impact of the 2017 Summer Universiade by examining the Taiwanese (the hosting country) residents’ willingness to attendance and estimated individual spending (consumption) while attending the games. Through a series of convenience sampling surveys from February to April, 2017, 1,021 respondents (53.5% males and 45.3% females) reported their potential personal spending, willingness to attend the events, and preferred ticket price range. The results indicated that 61.5% of respondents expressed their willingness to purchase tickets. Projected cost for each individual potential game attendee was about $58 USDs. The ideal general event ticket price should be set around$13.5 USDs. The estimated total revenues based on one million game attendees’ (including local and non-local Taiwanese residents and foreign visitors) personal spending reached $67-million USDs. This revenue figure was projected to generate an economic impact of$154.1-million USDs. The researchers further compared the obtained figures with the finding of past sport economic impact studies and addressed the significance and rationale for selecting a modest impact multiplier for the calculation. Additional recommendations are offered in using impact study results to plan strategies for boosting tourism and winning bids for future event

Projected Economic and Financial Benefits of the 2017 Summer Universiade Games.

In Western society, sport economic impact studies are often utilized to showcase the financial benefits for hosting mega sport event or to justify the spending for entering bids or covering infrastructure construction costs. This study projected economic impact of the 2017 Summer Universiade by examining the Taiwanese (the hosting country) residents’ willingness to attendance and estimated individual spending (consumption) while attending the games. Through a series of convenience sampling surveys from February to April, 2017, 1,021 respondents (53.5% males and 45.3% females) reported their potential personal spending, willingness to attend the events, and preferred ticket price range. The results indicated that 61.5% of respondents expressed their willingness to purchase tickets. Projected cost for each individual potential game attendee was about $58 USDs. The ideal general event ticket price should be set around$13.5 USDs. The estimated total revenues based on one million game attendees’ (including local and non-local Taiwanese residents and foreign visitors) personal spending reached $67-million USDs. This revenue figure was projected to generate an economic impact of$154.1-million USDs. The researchers further compared the obtained figures with the finding of past sport economic impact studies and addressed the significance and rationale for selecting a modest impact multiplier for the calculation. Additional recommendations are offered in using impact study results to plan strategies for boosting tourism and winning bids for future events