Change in Localization of Alkaline Phosphatase and Mannosidase II by Colchicine Treatment of Primary Cultures of Fetal Rat Hepatocytes

We examined the changes in localization of alkaline phosphatase (ALP) and mannosidase II (man II), a Golgi marker, after colchicine treatment of primary cultures of fetal rat hepatocytes, using double immunofluorescence staining and confocal laser microscopy. In hepatocytes cultured in basal medium, ALP was localized in the perinuclear cytoplasm, and man II was observed in the Golgi region of the cytoplasm. When hepatocytes were cultured in dexamethasone-supplemented medium, ALP was also localized in the plasma membrane surrounding the bile canaliculus-like structure that was formed between adjacent cells. In hepatocytes cultured in the same medium containing colchicine, the structure of microtubules in the cytoplasm was lost, man II exhibited granular distribution scattering throughout the cytoplasm, and ALP was localized in coarse granular sites of the cytoplasm. However, ALP was not colocalized at the same sites as man II. The present study indicated that colchicine inhibits the dexamethasone-promoted translocation of ALP to the plasma membrane surrounding the bile canaliculus-like structure in primary cultures of fetal rat hepatocytes by disassembling microtubules and discomposing the Golgi complex

Localization of Alkaline Phosphatase and Cathepsin D during Cell Restoration after Colchicine Treatment in Primary Cultures of Fetal Rat Hepatocytes

Localization of alkaline phosphatase (ALP) and cathepsin D (CAPD) in primary cultures of fetal rat hepatocytes was examined using double immunofluorescent staining in order to investigate the relationship between lysosome movement and the fate of ALP during cell restoration after microtubule disruption by colchicine. At 3 hr and 24 hr after colchicine treatment, numerous coarse dots containing ALP were observed throughout the cytoplasm, and some of these showed colocalization with CAPD. At 48 hr and 72 hr after colchicine treatment, although most of the dots containing ALP in the cytoplasm disappeared, dots containing CAPD remained. The present results suggest that the denatured ALP proteins remaining in the cytoplasm of hepatocytes during cell restoration after colchicine treatment are digested by lysosomes

Identification and frequency shaping control of a vibration isolation system

ArticleCONTROL ENGINEERING PRACTICE.16(6):711-723(2008)journal articl

Lack of evidence for a role of Epstein–Barr virus in the increase of lung cancer in idiopathic pulmonary fibrosis

AbstractIdiopathic pulmonary fibrosis (IPF) is known as an independent risk factor for lung cancer. Because Epstein–Barr virus (EBV) may be involved in the genesis of IPF as well as certain malignancies, we investigated whether EBV contributes to the increased incidence of lung cancer in IPF. The formalin-fixed and paraffin-embedded lung sections were prepared from 22 lung cancer patients with IPF and 22 lung cancer patients without IPF. All of the IPF patients pathologically showed usual interstitial pneumonia. In situ hybridization for EBV-encoded small non-polyadenylated RNAs failed to show positive signals in the cancer tissues of either IPF or non-IPF patients. This study did not provide evidence for an etiologic role of EBV in the development of lung cancer in IPF

Exponentially Increasing Incidences of Cutaneous Malignant Melanoma in Europe Correlate with Low Personal Annual UV Doses and Suggests 2 Major Risk Factors

For several decades the incidence of cutaneous malignant melanoma (CMM) steadily increased in fair-skinned, indoor-working people around the world. Scientists think poor tanning ability resulting in sunburns initiate CMM, but they do not understand why the incidence continues to increase despite the increased use of sunscreens and formulations offering more protection. This paradox, along with lower incidences of CMM in outdoor workers, although they have significantly higher annual UV doses than indoor workers have, perplexes scientists. We found a temporal exponential increase in the CMM incidence indicating second-order reaction kinetics revealing the existence of 2 major risk factors. From epidemiology studies, we know one major risk factor for getting CMM is poor tanning ability and we now propose the other major risk factor may be the Human Papilloma Virus (HPV) because clinicians find β HPVs in over half the biopsies. Moreover, we uncovered yet another paradox; the increasing CMM incidences significantly correlate with decreasing personal annual UV dose, a proxy for low vitamin D3 levels. We also discovered the incidence of CMM significantly increased with decreasing personal annual UV dose from 1960, when it was almost insignificant, to 2000. UV and other DNA-damaging agents can activate viruses, and UV-induced cytokines can hide HPV from immune surveillance, which may explain why CMM also occurs in anatomical locations where the sun does not shine. Thus, we propose the 2 major risk factors for getting CMM are intermittent UV exposures that result in low cutaneous levels of vitamin D3 and possibly viral infection

Universality of Bias- and Temperature-induced Dephasing in Ballistic Electronic Interferometers

We performed a transport measurement in a ballistic Aharonov-Bohm ring and a Fabry-Perot type interferometer. In both cases we found that the interference signal is reversed at a certain bias voltage and that the visibility decays exponentially as a function of temperature, being in a strong analogy with recent reports on the electronic Mach-Zehnder interferometers. By analyzing the data including those in the previous works, the energy scales that characterize the dephasing are found to be dominantly dependent on the interferometer size, implying the presence of a universal behavior in ballistic interferometers in both linear and non-linear transport regimes.Comment: 4 pages, 4 figures, accepted for publication in PRB rapid com

Two-Thirds Honest-Majority MPC for Malicious Adversaries at Almost the Cost of Semi-Honest

Secure multiparty computation (MPC) enables a set of parties to securely carry out a joint computation of their private inputs without revealing anything but the output. Protocols for semi-honest adversaries guarantee security as long as the corrupted parties run the specified protocol and ensure that nothing is leaked in the transcript. In contrast, protocols for malicious adversaries guarantee security in the presence of arbitrary adversaries who can run any attack strategy. Security for malicious adversaries is typically what is needed in practice (and is always preferred), but comes at a significant cost. In this paper, we present the first protocol for a two-thirds honest majority that achieves security in the presence of malicious adversaries at essentially the exact same cost as the best known protocols for semi-honest adversaries. Our construction is not a general transformation and thus it is possible that better semi-honest protocols will be constructed which do not support our transformation. Nevertheless, for the current state-of-the-art for many parties (based on Shamir sharing), our protocol invokes the best semi-honest multiplication protocol exactly once per multiplication gate (plus some additional local computation that is negligible to the overall cost). Concretely, the best version of our protocol requires each party to send on average of just $2\frac23$ elements per multiplication gate (when the number of multiplication gates is at least the number of parties). This is four times faster than the previous-best protocol of Barak et al. (ACM CCS 2018) for small fields, and twice as fast as the previous-best protocol of Chida et al. (CRYPTO 2018) for large fields

PCLO rs2522833 impacts HPA system activity in healthy young adults

Recent genetic studies showed evidence for a role of the single-nucleotide polymorphism rs2522833 within the PCLO gene in the etiology of major depression, and rs2522833 has been shown to modulate hypothalamic pituitary adrenal (HPA) axis activity during antidepressant treatment. Monoaminergic modulation of the HPA system may be one possible pathomechanism by which PCLO exerts its effect on depression. In the present study, we investigated the effect of rs2522833 on the cortisol awakening response (CAR) in healthy young adults. A total of 66 healthy volunteers from the community (36 men and 30 women) aged 18–25 years without individual or family history of affective disorders and schizophrenia collected saliva cortisol samples at 0, 30, 45 and 60 min after awakening on two consecutive working days. We identified a blunted CAR (AUCinc) in rs2522833 risk-allele (C) carriers, possibly indicating exhausted regulatory mechanisms underlying the HPA system. We also identified higher neuroticism scores in rs2522833 risk-allele carriers but no phenotypic correlation between the CAR (AUCinc) and neuroticism. These findings suggest that the rs2522833 risk variant might increase vulnerability to depression both by physiological and behavioral pathways, which appear, however, not to be substantially overlapped. Replication with larger samples is warranted