Amplification parameters for the qPCR assay applied to artificial formalin-fixed samples.

<p>The values of Efficiency, Slope and Intercept are expressed as mean ± standard deviation calculated from two spiking experiments with <i>V</i>. <i>cholerae</i> ATCC 39315 cells, each quantified in triplicate on the same run (n = 6)</p><p>Amplification parameters for the qPCR assay applied to artificial formalin-fixed samples.</p

<i>V</i>. <i>cholerae</i> detection in CPR samples from endemic Cholera regions (South West Africa).

<p>Relative abundance of <i>Vibrio</i> spp. and <i>Vibrio cholerae</i> in CPR samples collected in cholera endemic areas of the Benguela Current Large Marine Ecosystem region (BCLME, South West Africa) as a proof of concept for the possible use of the CPR technology and the developed qPCR assay in cholera studies (see main text for details).</p

Performance of the qPCR assay for detection of <i>V</i>. <i>cholerae</i> in artificially degraded DNA samples.

<p>(A) Electropherogram plot obtained by Agilent Bioanalyzer analysis of artificially fragmented genomic DNA of <i>V</i>. <i>cholerae</i> ATCC 39315. (B) Plot of mean Cq-values from three replicates tested against the <i>V</i>. <i>cholerae</i> artificially fragmented DNA inputs. Error bars indicate the standard deviations of the means.</p

Sensitivity of the qPCR assay for detection of <i>V</i>. <i>cholerae</i> O1 El Tor N16961.

<p>DNA was amplified with the gbpA TaqMan primers in the presence of the gbpA fluorogenic probe. (A) Amplification plot of <i>V</i>. <i>cholerae</i> ATCC 39315 sample dilutions containing 10⁶, 10⁵, 10⁴, 10³, 10², 10¹ genome copies per reaction (GU/rx). (B) Plot of mean Cq-values from three replicates tested against the <i>V</i>. <i>cholerae</i> DNA inputs. Error bars indicate the standard deviations of the means.</p

qPCR detection of <i>V</i>. <i>cholerae</i> ATCC 39315 cells in mixed cultures containing <i>V</i>. <i>mimicus</i> UM 6812.

<p>qPCR detection of <i>V</i>. <i>cholerae</i> ATCC 39315 cells in mixed cultures containing 0.5 ng/rx (corresponding to 8x10⁴ GU/rx) of <i>V</i>. <i>mimicus</i> UM 6812. Quantification cycle (Cq) is expressed as mean ± standard deviation calculated from two spiking experiments each quantified in triplicate on the same run (n = 6)</p><p>qPCR detection of <i>V</i>. <i>cholerae</i> ATCC 39315 cells in mixed cultures containing <i>V</i>. <i>mimicus</i> UM 6812.</p

Amplification parameters for the qPCR assay applied to environmental and stool samples.

<p>The values of Efficiency, Slope and Intercept are expressed as mean ± standard deviation calculated from two spiking experiments with <i>V</i>. <i>cholerae</i> ATCC 39315 cells, each quantified in triplicate on the same run (n = 6)</p><p>Amplification parameters for the qPCR assay applied to environmental and stool samples.</p