The lipid phase transition of <i>Juncea fragilis, J. juncea</i>, and <i>Ellisella robusta</i> oocytes at early (1a, c, and e, respectively) and late (1b, d, and f, respectively) stages, as determined from a Fourier Transform Infrared analyzer.

<p>The inflection point of the lipid phase transition curve from liquid crystalline to gel phase represents the the lipid phase transition temperature.</p

The lipid phase transition temperature (LPTT) of early and late stages oocytes of <i>Juncea fragilis</i> (a), <i>J. juncea</i> (b), and <i>Ellisella robusta</i>.

<p>Error bars indicate standard error of the means. Astrices represent significant difference between early and late stage at the same chilling time period (<i>p<</i>0.05).</p

The lipid phase transition temperature (LPTT) of <i>Juncea fragilis</i>, <i>J. juncea</i>, and <i>Euplexaura. robusta</i> oocytes at early (a) and late (b) developmental stages after chilling at 5°C for up to 144 h in filtered seawater.

<p>Error bars indicate standard error of the means. Astrices represent significant differences (<i>p</i><0.05) between LPTTs of <i>J. fragilis, J. juncea</i>, and <i>E. robusta</i> oocytes at the same chilling time period.</p

Gradient elution program for HPLC-ELSD separation.

<p>Gradient elution program for HPLC-ELSD separation.</p

Normalized ATP concentrations of <i>Junceella juncea</i> oocytes after vitrification in PG-based VS2 for 2 min.

<p>Error bars represent standard errors. The bars with different letters are significantly different (P < 0.05). Trial 1: PG-based ES1 for 15mins, PG-based ES2 for 10mins; Trial 2: PG-based ES1 for 15 mins, PG-based ES2 for 5 mins; Trial 3: PG-based ES1 for 10mins, PG-based ES2 for 10mins; Trial 4: PG-based ES1 for 10mins, PG-based ES2 for 5mins.</p

VSs of PG and glycerol are synthesized with EG and methanol at different concentrations.

<p>* Ten solutions in total are formed using PG and glycerol separately, both contain the same concentrations.</p><p>VSs of PG and glycerol are synthesized with EG and methanol at different concentrations.</p

Wax ester (WE), triacylglycerol (TAGs), total fatty acid (TFA), phosphatidyethanolamine (PE) and phosphatidylcholine (PC) content of oocyte of two gorgorian corals.

<p>Data are % composition of total lipid.</p

The composition of lipid content in early (a) and late (b) stage oocytes of <i>J. juncea</i> and <i>J. fragilis</i> oocytes.

<p>Error bars indicate standard errors of the means. Asterisks represent significant difference of the same lipid category between <i>J. juncea</i> and <i>J. fragilis</i> oocytes (<i>p<0.05</i>).</p

Flow chart of the experimental procedures.

<p>Flow chart of the experimental procedures.</p

The distribution of total lipid in early and late stage oocytes of <i>J. juncea</i> and <i>J. fragilis.</i>

<p>Error bars indicate standard errors of the means. Asterisks represent significant difference between groups (<i>p<0.05</i>).</p