Distribution of (A) normalized Put, (B) normalized Spd, (C) normalized Spm values in PCa, BPH and HC.

<p>The black bar in the figures indicates the mean value of each subset while the error bar indicates the corresponding SEM.</p

Overlaid UPLC-MS/MS SRM chromatograms of 1000 ppb mixed polyamines standard (0–10 mins being shown).

<p>Put (Black peak, t_R = 4.3 min), Put-d₈ (Pink peak, t_R = 4.3 min), Spd (Blue peak, t_R = 6.6 min), Spd-d₈ (Red peak, t_R = 6.6 min), Spm (Yellow peak, t_R = 7.9 min) and Spm-d₈ (Green peak, t_R = 7.9 min).</p

Column statistics of normalized polyamine contents (μmol/g of creatinine) in different subsets.

<p>Column statistics of normalized polyamine contents (μmol/g of creatinine) in different subsets.</p

Receiver operating characteristic analysis for normalized Put, Spd and Spm values.

<p>Receiver operating characteristic analysis for normalized Put, Spd and Spm values.</p

Clinicopathologic characteristics of patients.

<p>Clinicopathologic characteristics of patients.</p

Polyamine metabolic pathway (focusing on Put, Spd and Spm only).

<p>Polyamine metabolic pathway (focusing on Put, Spd and Spm only).</p

Comparative Studies of the Cellular Uptake, Subcellular Localization, and Cytotoxic and Phototoxic Antitumor Properties of Ruthenium(II)–Porphyrin Conjugates with Different Linkers

Six water-soluble free-base porphyrin-Ru­(II) conjugates, <b>1</b>–<b>3</b>, and Zn­(II) porphyrin-Ru­(II) conjugates, <b>4</b>–<b>6</b>, with different linkers between the hydrophobic porphyrin moiety and the hydrophilic Ru­(II)-polypyridyl complex, have been synthesized. The linear and two-photon-induced photophysical properties of these conjugates were measured and evaluated for their potential application as dual in vitro imaging and photodynamic therapeutic (PDT) agents. Conjugates <b>1</b>–<b>3</b>, with their high luminescence and singlet oxygen quantum yields, were selected for further study of their cellular uptake, subcellular localization, and cytotoxic and photocytotoxic (under linear and two-photon excitation) properties using HeLa cells. Conjugate <b>2</b>, with its hydrophobic phenylethynyl linker, was shown to be highly promising for further development as a bifunctional probe for two-photon (NIR) induced PDT and in vitro imaging. Cellular uptake and subcellular localization properties were shown to be crucial to its PDT efficacy

Gallium and Functionalized-Porphyrins Combine to Form Potential Lysosome-Specific Multimodal Bioprobes

A water-soluble bimetallic normal (“cold”) and radiochemical (“hot”) gallium–porphyrin–ruthenium–bipyridine complex (<b>GaporRu-1</b>) has been synthesized by microwave methodology in short reaction times with good (>85%) yields. ^<b>68</b><b>GaporRu-1</b> is demonstrated to be a potential multimodal and functional bioprobe for positron emission tomography (PET), lysosome specific optical imaging, and photodynamic therapy