Pyrazine: Supercollisions or Simple Reactions?

[[sponsorship]]原子與分子科學研究所[[note]]已出版;[SCI];有審查制度;具代表性[[note]]http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Drexel&SrcApp=hagerty_opac&KeyRecord=0021-9606&DestApp=JCR&RQ=IF_CAT_BOXPLO

A comparison of anionic nanoparticles and microparticles as vaccine delivery systems.

The objective of this work was to conduct an in vivo comparison of nanoparticles and microparticles as vaccine delivery systems. Poly (lactide-co-glycolide) (PLG) polymers were used to create nanoparticles size 110 nm and microparticles of size 800-900 nm. Protein antigens were then adsorbed to these particles. The efficacy of these delivery systems was tested with two protein antigens. A recombinant antigen from Neisseria meningitides type B (MenB) was administered intramuscularly (i.m.) or intraperitonealy (i.p.). An antigen from HIV-1, env glycoprotein gp140 was administered intranasally (i.n.) followed by an i.m. boost. From three studies, there were no differences between the nanoparticles and micro-particles formulations. Both particles led to comparable immune responses in mice. The immune responses for MenB (serum bactericidal activity and antibody titers) were equivalent to the control of aluminum hydroxide. For the gp140, the LTK63 was necessary for high titers. Both nanoparticles and microparticles are promising delivery systems

The potency of the adjuvant, CpG oligos, is enhanced by encapsulation in PLG microparticles.

The objective of this work was to evaluate the potency of the CpG containing oligonucleotide encapsulated within poly(lactide-co-glycolide), and coadministered with antigen adsorbed to poly(lactide-co-glycolide) microparticles (PLG particles). The formulations evaluated include, CpG added in soluble form, CpG adsorbed, and CpG encapsulated. The antigen from Neisseria meningitidis serotype B (Men B) was used in these studies. The immunogenicity of these formulations was evaluated in mice. Poly(lactide-co-glycolide) microparticles were synthesized by a w/o/w emulsification method in the presence of a charged surfactant for the formulations. Neisseria meningitidis B protein was adsorbed to the PLG microparticles, with binding efficiency and initial release measured. CpG was either added in the soluble or adsorbed or encapsulated form based on the type of formulation. The binding efficiency, loading, integrity and initial release of CpG and the antigen were measured from all the formulations. The formulations were then tested in mice for their ability to elicit antibodies, bactericidal activity and T cell responses. Encapsulating CpG within PLG microparticles induced statistically significant higher antibody, bactericidal activity and T cell responses when compared to the traditional method of delivering CpG in the soluble form

UV photodissociation dynamics of allyl radical by photofragment translational spectroscopy

Photodissociation of the allyl radical, CH2CHCH2, has been studied using the method of molecular beam photofragment translational spectroscopy following excitation to the (C) over tilde(2 B-2(1)) and (A) over tilde(1 B-2(1)) states by 248 and 351 nm photons. Two different primary channels have been detected following 248 nm excitation: H-atom loss (84%) and CH3 elimination (16%). From the product translational energy distribution and polarization dependence studies, dissociation processes from the ground-state C3H5 potential energy surface are inferred for both wavelengths. At 248 nm there may also be a contribution to the H-atom loss channel from predissociation by a higher electronically excited state. Rice-Ramsperger-Kassel-Marcus (RRKM) calculations show that the formation of cyclopropene is not important, while formation of allene and methylacetylene from dissociation of 1- and 2-propenyl radicals are important reaction pathways at both wavelengths. Translational energy distributions peaking well away from zero provide evidence for CH3 elimination directly from an allylic structure through a four-member cyclic transition state. (C) 1998 American Institute of Physics. [S0021-9606(98)02137-0]

Characterization of antigens adsorbed to anionic PLG microparticles by XPS and TOF-SIMS.

The chemical composition of the surface of anionic PLG microparticles before and after adsorption of vaccine antigens was measured using X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (TOF-SIMS). The interfacial distributions of components will reflect underlying interactions that govern properties such as adsorption, release, and stability of proteins in microparticle vaccine delivery systems. Poly(lactide-co-glycolide) microparticles were prepared by a w/o/w emulsification method in the presence of the anionic surfactant dioctyl sodium sulfosuccinate (DSS). Ovalbumin, lysozyme, a recombinant HIV envelope glyocoprotein and a Neisseria meningitidis B protein were adsorbed to the PLG microparticles, with XPS and time-of-flight secondary mass used to analyze elemental and molecular distributions of components of the surface of lyophilized products. Protein (antigen) binding to PLG microparticles was measured directly by distinct elemental and molecular spectroscopic signatures consistent with amino acids and excipient species. The surface sensitive composition of proteins also included counter ions that support the importance of electrostatic interactions being crucial in the mechanism of adsorptions. The protein binding capacity was consistent with the available surface area and the interpretation of previous electron and atomic force microscope images strengthened by the quantification possible by XPS and the qualitative identification possible with TOF-SIMS. Protein antigens were detected and quantified on the surface of anionic PLG microparticles with varying degrees of efficiency under different adsorption conditions such as surfactant level, pH, and ionic strength. Observable changes in elemental and molecular composition suggest an efficient electrostatic interaction creating a composite surface layer that mediates antigen binding and release

PHOTODISSOCIATION OF OZONE AT 193 NM BY HIGH-RESOLUTION PHOTOFRAGMENT TRANSLATIONAL SPECTROSCOPY

The photodissociation of ozone has been studied at 193 nm using high resolution photofragment translational spectroscopy. The results show six distinct peaks in the time-of-flight spectra for the O2 product and its momentum-matched O atom counterpart. The translational energy distributions determined from the time-of-flight spectra reveal the production of a range of electronic states of the photofragments. The product electronic states were identified based on the translational energy distributions, with the aid of state-resolved imaging experiments by Houston and co-workers. The results reveal the production of a substantial yield of highly excited triplet states of O2 , recently suggested to play an important role in the stratospheric ozone balance. In addition, peaks corresponding to O2(a 1Dg) and O2(b 1Sg 1) were observed, the latter confirming a previous report @A. A. Turnipseed et al., J. Chem. Phys. 95, 3244 ~1991!#. Evidence was seen for a small contribution from the triple dissociation O3!3O(3P), and insight into the dissociation dynamics for this process was inferred from the translational energy distributions. Branching fractions and angular distributions were measured for all channels. The latter were found in general to yield negative b parameters, in contrast to what is seen at longer wavelengths