Specificity of the BFV nested Real-Time RT- qPCR assay.

<p>Specificity of the BFV nested Real-Time RT- qPCR assay.</p

Oligonucleotide primer sequences used in the nested RT-PCR assay.

*<p>Genbank Accession number U73745.1.</p

Limit of Detection of the BFV real-time qPCR nested assay.

*<p>P = 0.0033, F = 8.1.</p

Seasonal activity, vector relationships and genetic analysis of mosquito-borne Stratford virus - Fig 2

<p>Phylogenetic association of deduced amino acids of the partial sequence of the NS5 gene region of STRV isolates (n = 43), STRV C338 ●, TS5273, KOKV AusMRM32 ▲, Bainyik MK7979 and New Mapoon CY1014 virus strain. The percentage association is shown above the branches with branch lengths measured in the number of substitutions per site indicated by the scale bar.</p

Coordinates of mosquito collection sites on the NSW coast where Stratford virus was isolated, 1995–2013.

<p>Locations are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0173105#pone.0173105.t002" target="_blank">Table 2</a>.</p

Phylogenetic association of STRV isolates (n = 43) and STRV C388 GenBank prototypes (A) and the association between STRV isolates, TS5273, KOKV AusMRM32, Banyik MK7979 and the New Mapoon CY1014 virus strain (B).

<p>The trees are based on the nucleic acid partial sequence of the NS5 gene region (556 bp). The posterior probability values are shown at the nodes with the branch lengths measured in the number of substitutions per site indicated by the scale bar. Abbreviations used: BN = Blacktown; CC = Central Coast; LM = Lake Macquarie; ByB = Byron Bay; PE = Penrith; PTS = Port Stephens; GR = Georges River; BB = Batemans Bay; SOP = Sydney Olympic Park; HW = Hawkesbury; PA = Parramatta; WPH = West Pennant Hills.</p

The total number (n = 43) of Stratford virus isolates recorded by location and mosquito species from 1995 through 2013.

<p>The total number (n = 43) of Stratford virus isolates recorded by location and mosquito species from 1995 through 2013.</p