Titer of ZIKV in the salivary glands of <i>Ae. aegypti</i> at different days post-infectious bloodmeal.

<p>The bar indicates median viral titers and limit of detection is represented by broken lines. A significant increase(*) (P<0.001) in mean viral titer was observed between days 7 and 10 pe.</p

Midguts and salivary glands infection rates of ZIKV in <i>Ae. aegypti</i> at different days post-infectious bloodmeal.

<p>Eight mosquitoes were sampled per day.</p

Titer of ZIKV in midguts of <i>Ae. aegypti</i> at different days post-infectious bloodmeal.

<p>The bar indicates median viral titers and limit of detection is represented by broken lines. A significant increase(*) (P = 0.026) in mean viral titer was observed between days 3 and 5 pe.</p

<i>w</i>Mel reduces midgut CHIKV infection in Singapore’s <i>Ae</i>. <i>aegypti</i>.

<p>Midgut CHIKV titre in WT and wMel-Sg <i>Ae</i>. <i>aegypti</i> at days 6, 9 and 13 post-infections. CHIKV levels in midguts were determined using qRT-PCR assay and expressed as Log10 RNA copies/ml. Bars denote median viral titres. *** denotes significant difference at P<0.05 by Mann-Whitney test. Each point represents an individual midgut.</p

Infection and dissemination rates for ZIKV and CHIKV among WT and <i>w</i>Mel-Sg <i>Ae</i>. <i>aegypti</i> strains at days 6-, 9- and 13-days infectious blood meal.

<p>Infection and dissemination rates for ZIKV and CHIKV among WT and <i>w</i>Mel-Sg <i>Ae</i>. <i>aegypti</i> strains at days 6-, 9- and 13-days infectious blood meal.</p

<i>w</i>Mel inhibits CHIKV salivary glands dissemination in <i>Ae</i>. <i>aegypti</i>.

<p>Salivary glands CHIKV titre in WT and wMel-Sg <i>Ae</i>. <i>aegypti</i> at days 6, 9 and 13 post-infections. CHIKV levels in both salivary glands were determined salivary glands using viral titration assay and expressed as Log₁₀TCI₅₀/mL. Bars denote median viral titres. *** denotes significant difference at P<0.05 by Mann-Whitney test. Each point represents an individual salivary glands.</p

<i>w</i>Mel inhibit the dissemination of ZIKV infection in Singapore’s <i>Ae</i>. <i>aegypti</i>.

<p>Titre of ZIKV in midguts and salivary glands of <i>w</i>Mel-Sg and WT <i>Ae</i>. <i>aegypti</i> at days 6, 9 and 13 post-infectious blood meal. ZIKV level is both midguts and salivary glands were determined using viral titration assay and expressed as Log₁₀TCI₅₀/mL. Bars denote median viral titres. *** denotes significant difference at P<0.05 by Mann-Whitney test. Each point represents an individual midgut/salivary glands.</p