Sublingual Immunization with M2-Based Vaccine Induces Broad Protective Immunity against Influenza

The ectodomain of matrix protein 2 (M2e) of influenza A virus is a rationale target antigen candidate for the development of a universal vaccine against influenza as M2e undergoes little sequence variation amongst human influenza A strains. Vaccine-induced M2e-specific antibodies (Abs) have been shown to display significant cross-protective activity in animal models. M2e-based vaccine constructs have been shown to be more protective when administered by the intranasal (i.n.) route than after parenteral injection. However, i.n. administration of vaccines poses rare but serious safety issues associated with retrograde passage of inhaled antigens and adjuvants through the olfactory epithelium. In this study, we examined whether the sublingual (s.l.) route could serve as a safe and effective alternative mucosal delivery route for administering a prototype M2e-based vaccine. The mechanism whereby s.l. immunization with M2e vaccine candidate induces broad protection against infection with different influenza virus subtypes was explored.A recombinant M2 protein with three tandem copies of the M2e (3M2eC) was expressed in Escherichia coli. Parenteral immunizations of mice with 3M2eC induced high levels of M2e-specific serum Abs but failed to provide complete protection against lethal challenge with influenza virus. In contrast, s.l. immunization with 3M2eC was superior for inducing protection in mice. In the latter animals, protection was associated with specific Ab responses in the lungs.The results demonstrate that s.l. immunization with 3M2eC vaccine induced airway mucosal immune responses along with broad cross-protective immunity to influenza. These findings may contribute to the understanding of the M2-based vaccine approach to control epidemic and pandemic influenza infections

Search for D0D^{0} decays to invisible final states at Belle

We report the result from the first search for $D^0$ decays to invisible final states. The analysis is performed on a data sample of 924 $\rm{fb}^{-1}$ collected at and near the $\Upsilon(4S)$ and $\Upsilon(5S)$ resonances with the Belle detector at the KEKB asymmetric-energy $e^{+}e^{-}$ collider. The absolute branching fraction is determined using an inclusive $D^0$ sample, obtained by fully reconstructing the rest of the particle system including the other charmed particle. No significant signal yield is observed and an upper limit of $9.4\times 10^{-5}$ is set on the branching fraction of $D^0$ to invisible final states at 90\% confidence level.Comment: 17 pages, 4 figures, submitted to PRD(RC

Search for CPCP violation in the D+→π+π0D^{+}\to\pi^{+}\pi^{0} decay at Belle

We search for $CP$ violation in the charged charm meson decay $D^{+}\to\pi^{+}\pi^{0}$, based on a data sample corresponding to an integrated luminosity of $\rm 921~fb^{-1}$ collected by the Belle experiment at the KEKB $e^{+}e^{-}$ asymmetric-energy collider. The measured $CP$ violating asymmetry is $[+2.31\pm1.24({\rm stat})\pm0.23({\rm syst})]\%$, which is consistent with the standard model prediction and has a significantly improved precision compared to previous results.Comment: 8 pages, 3 figure

First Observation of Doubly Cabibbo-Suppressed Decay of a Charmed Baryon: Λc+→pK+π−\Lambda^{+}_{c} \rightarrow p K^{+} \pi^{-}

We report the first observation of the decay $\Lambda^{+}_{c} \rightarrow p K^{+} \pi^{-}$ using a 980 $\mathrm{fb^{-1}}$ data sample collected by the Belle detector at the KEKB asymmetric-energy $e^{+}e^{-}$ collider. This is the first doubly Cabibbo-suppressed decay of a charmed baryon to be observed. We measure the branching ratio of this decay with respect to its Cabibbo-favored counterpart to be $\mathcal{B}(\Lambda^{+}_{c} \rightarrow p K^{+} \pi^{-})/\mathcal{B}(\Lambda^{+}_{c} \rightarrow p K^{-} \pi^{+})=(2.35\pm0.27\pm0.21)\times10^{-3}$, where the uncertainties are statistical and systematic, respectively.Comment: 6 pages, 3 figure

Evidence for Isospin Violation and Measurement of CPCP Asymmetries in B→K∗(892)γB \to K^{\ast}(892) \gamma

We report the first evidence for isospin violation in $B \to K^* \gamma$ and the first measurement of difference of $CP$ asymmetries between $B^+ \to K^{*+} \gamma$ and $B^0 \to K^{*0} \gamma$. This analysis is based on the data sample containing $772 \times 10^6 B\bar{B}$ pairs that was collected with the Belle detector at the KEKB energy-asymmetric $e^+ e^-$ collider. We find evidence for the isospin violation with a significance of 3.1$\sigma$, $\Delta_{0+} = (+6.2 \pm 1.5 ({\rm stat.}) \pm 0.6 ({\rm syst.}) \pm 1.2 (f_{+-}/f_{00}))$\%, where the third uncertainty is due to the uncertainty on the fraction of $B^+B^-$ to $B^0\bar{B}^0$ production in $\Upsilon(4S)$ decays. The measured value is consistent with predictions of the SM. The result for the difference of $CP$ asymmetries is $\Delta A_{CP} = (+2.4 \pm 2.8({\rm stat.}) \pm 0.5({\rm syst.}))$\%, consistent with zero. The measured branching fractions and $CP$ asymmetries for charged and neutral $B$ meson decays are the most precise to date. We also calculate the ratio of branching fractions of $B^0 \to K^{*0} \gamma$ to $B_s^0 \to \phi \gamma$.Comment: 11 pages, 7 figures. shown at FPCP2017. accepted by PR

Search for B→hννˉ\boldsymbol{B\to h\nu\bar{\nu}} decays with semileptonic tagging at Belle

We present the results of a search for the rare decays $B\to h\nu\overline{\nu}$, where $h$ stands for $K^+,\:K^0_{\mathrm{S}},\:K^{\ast +},\:K^{\ast 0},\:\pi^+,\:\pi^0,\:\rho^+$ and $\rho^{0}$. The results are obtained with $772\times10^{6}$ $B\overline{B}$ pairs collected with the Belle detector at the KEKB $e^+ e^-$ collider. We reconstruct one $B$ meson in a semileptonic decay and require a single $h$ meson but nothing else on the signal side. We observe no significant signal and set upper limits on the branching fractions. The limits set on the $B\to K^0_{\mathrm{S}}\nu\overline{\nu}$, $B^0\to K^{*0}\nu\overline{\nu}$, $B\to \pi^+\nu\overline{\nu}$, $B^0\to\pi^0\nu\overline{\nu}$, $B^+\to\rho^+\nu\overline{\nu}$, and $B^0\to\rho^0\nu\overline{\nu}$ channels are the world's most stringent.Comment: Submitted to PR

Search for a dark vector gauge boson decaying to π+π−\pi^+ \pi^- using η→π+π−γ\eta \rightarrow \pi^+\pi^- \gamma decays

We report a search for a dark vector gauge boson $U^\prime$ that couples to quarks in the decay chain $D^{*+} \to D^0 \pi^+, D^0 \to K^0_S \eta, \eta \to U^\prime \gamma$, $U^\prime \to \pi^+ \pi^-$. No signal is found and we set a mass-dependent limit on the baryonic fine structure constant of $10^{-3} - 10^{-2}$ in the $U^\prime$ mass range of 290 to 520 MeV/$c^2$. This analysis is based on a data sample of 976 fb$^{-1}$ collected by the Belle experiment at the KEKB asymmetric-energy $e^+e^-$ collider.Comment: 6 pages, 4 figure