Prognosis of hepatocellular carcinoma patients with bile duct tumor thrombus after hepatic resection or liver transplantation in Asian populations: A meta-analysis

<div><p>Background</p><p>Hepatocellular carcinoma (HCC) with bile duct tumor thrombus (BDTT) in the clinic is rare, and surgical treatment is currently considered the most effective treatment. However, the influence of BDTT on the prognosis of HCC patients who underwent surgery remains controversial in previous studies. Therefore, this paper uses meta-analysis method to elucidate this controversy.</p><p>Methods</p><p>In this study, we conducted a literature search on databases PubMed, Embase and Web of Science from inception until September 2016. Each study was evaluated with Newcastle-Ottawa Scale (NOS). The pooled effect was calculated, and the association between BDTT and overall survival (OS) or disease-free survival (DFS) was reevaluated using meta-analysis for hazard ratio (HR) and 95% confidence interval (CI).</p><p>Results</p><p>A total of 11 studies was included containing 5295 patients. The (HR) for OS and DFS was 3.21 and 1.81, 95%CI was 2.34–4.39 and 1.17–2.78 respectively.</p><p>Conclusions</p><p>The results showed that HCC patients with BDTT had a worse prognosis than those without BDTT after hepatic resection or liver transplantation (LT).</p></div

Funnel plots for the evaluation of potential publication bias.

<p>Funnel plots for the evaluation of potential publication bias.</p

Flow diagram of the study selection process.

<p>Flow diagram of the study selection process.</p

Baseline characteristics for studies included in meta-analysis.

<p>Baseline characteristics for studies included in meta-analysis.</p

Sensitivity analysis for the evaluation of heterogeneity.

<p>Sensitivity analysis for the evaluation of heterogeneity.</p

Forest plots of studies evaluating hazard ratios of hepatocellular carcinoma (HCC) with bile duct tumor thrombus (BDTT) after hepatic resection or liver transplantation (LT) for overall survival or disease-free survival.

<p>Forest plots of studies evaluating hazard ratios of hepatocellular carcinoma (HCC) with bile duct tumor thrombus (BDTT) after hepatic resection or liver transplantation (LT) for overall survival or disease-free survival.</p

MOESM3 of EZH2 promotes DNA replication by stabilizing interaction of POLδ and PCNA via methylation-mediated PCNA trimerization

Additional file 3: Figure S3. Uncut immunoblots from the immunoprecipitation experiment

MOESM2 of EZH2 promotes DNA replication by stabilizing interaction of POLδ and PCNA via methylation-mediated PCNA trimerization

Additional file 2: Figure S2 (related to Fig. 2). A: Flow cytometry shows the cell cycle distribution of hDPCs from one donor at the indicated time points after release from serum deprivation. B: Immunoblot demonstrates the expression of CYCLIN D1 and CYCLIN A2, indicating G1 phase and S phase, respectively. α-TUBULIN was used a loading control of whole lysate. C: Statistical analysis of the EZH2-positive ratio (Left, n = 3) and Pearson’s correlation coefficient of EZH2 with DAPI (right, each dot represents one cell). D: Statistical analysis of the PLA-positive ratio (left, n = 5). The PLA signals are associated with DAPI on a single-cell basis (***P < 0.001

Forest plots of HAM-A psychic and somatic anxiety factor scores.

<p>(A) Baseline psychic anxiety factor score. (B) Baseline somatic anxiety factor score.</p

Wnt5a promotes the adhesion but inhibits the migration of hDPCs.

<p>A: A total of 25,000 cells were seeded for the indicated times and nonadherent cells were rinsed off. Adherent cells were stained with crystal violet and were analyzed spectrophotometrically. The number of adherent cells is shown as mean±SD from three independent experiments, with the number of DMEM-treated cells set as 100%. B: Confluent monolayers of hDPCs were scratched with a pipette tip and cultured in different medium containing 5% FBS for 12 hr. The relative migration distance of the wound edge was shown as mean±SD of three independent experiments, with the migration distance of DMEM-treated cells set as 100%. Bars, 100 µm. C: HDPCs were plated on glass coverslips coated with type I collagen and cultured with different medium for the indicated times. For FACs immunostaining, anti-vinculin antibody was used, and for F-actin staining, rhodamine-phalloidin was used, arrowheads mark FACs. Bars,10 µm. D: Confluent hDPCs were incubated with Wnt5a for the indicated times and Western analyses were used to detect the expression of vinculin, phospho-paxillin, phospho-MLC, with GAPDH, total-paxillin and total-MLC as loading control. The relative protein expression at 0 min is designated 1.0. *<i>p</i> < 0.05, n=3.</p