Characteristics of immune cell infiltration in inflamed mucosa of ulcerative colitis patients, hub gene candidates and key pathways in intestinal macrophage.

Tissue RNA was isolated from fresh mouse colon sample by Trizol reagent. Total RNA (1 µg) was reverse-transcribed using a reverse transcriptase kit (TakaRa, cat. RR036A-1, Tokyo, Japan). Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnolog, cat. 11201ES03, Shanghai, China) was used for Real-time qPCR. The comparative cycle threshold (Ct) method (2−ΔΔCT) was used to calculate relative expression, β-actin was used as the internal reference. We evaluated immunostaining by multiplying the intensity score and proportion score. Intensity score according to the intensity of cell staining: 0 for no positive staining (negative), 1 for light yellow (weakly positive), 2 for brown (positive) and 3 for dark brown (strongly positive). Proportion score according to the percentage of positive cells: 1 for ≤25%, 2 for 26%-50%, 3 for 51%-75% and 4 points for > 75%.</p

<b>Association between maternal thyroid function in early pregnancy and hypertensive disorders of pregnancy: a prospective cohort study</b>

Context: Hypertensive disorders of pregnancy (HDP) are linked with a variety of maternal and fetal complications. Numerous observational studies have examined the correlation between thyroid function and the incidence of HDP, with conflicting results. Objective: Our aim was to explore the correlation between thyroid function tests in early pregnancy, and the risk of HDP. Design: This research constitutes a prospective cohort study based on the China Birth Cohort Study (CBCS), from February 2018 to December 2020. Setting: A tertiary maternal and child health hospital. Participants: A total of 36,256 subjects from February 2018 to December 2020 were selected based on CBCS. Main Outcome Measures: Hypertensive disorders of pregnancy. Results: After application of the exclusion criteria, the final study population was 24,364 pregnant women and the prevalence of HDP was 8.77%. In the fully adjusted model (Model 3), thyroid-stimulating hormone (TSH) was significantly and positively correlated with HDP (odds ratio (OR) 1.034, 95%CI 1.007,1.063). Compared to the lowest quartile(reference), both the highest full-range and normal-range TSH quartiles significantly increased the risk of HDP (OR 1.219, 95% CI 1.072,1.387; OR 1.241, 95% CI 1.087,1.417, respectively), and all P for trend<.001. Based on restricted cubic spline, we identified a nearly linear correlation between the full range of TSH and risk of incident HDP (P for overall < 0.001, P for nonlinear=0.065). No significant association were found between free thyroxine (FT4), thyroid peroxidase antibody (TPOAb) positivity and the prevalence of HDP. Conclusions: High TSH might be associated with increased risk of HDP.</p