Identifying Functional Genes Influencing Gossypium hirsutum Fiber Quality

Fiber quality is an important economic index and a major breeding goal in cotton, but direct phenotypic selection is often hindered due to environmental influences and linkage with yield traits. A genome-wide association study (GWAS) is a powerful tool to identify genes associated with phenotypic traits. In this study, we identified fiber quality genes in upland cotton (Gossypium hirsutum L.) using GWAS based on a high-density CottonSNP80K array and multiple environment tests. A total of 30 and 23 significant single nucleotide polymorphisms (SNPs) associated with five fiber quality traits were identified across the 408 cotton accessions in six environments and the best linear unbiased predictions, respectively. Among these SNPs, seven loci were the same, and 128 candidate genes were predicted in a 1-Mb region (±500 kb of the peak SNP). Furthermore, two major genome regions (GR1 and GR2) associated with multiple fiber qualities in multiple environments on chromosomes A07 and A13 were identified, and within them, 22 candidate genes were annotated. Of these, 11 genes were expressed [log2(1 + FPKM)>1] in the fiber development stages (5, 10, 20, and 25 dpa) using RNA-Seq. This study provides fundamental insight relevant to identification of genes associated with fiber quality and will accelerate future efforts toward improving fiber quality of upland cotton

Tribology and Rheology of Polypropylene Grease with MoS₂ and ZDDP Additives at Low Temperatures

Polypropylene (PP) grease is a type of lubricating grease with excellent low-temperature performance. The wear and friction performance of steel/steel couples lubricated with PP grease containing molybdenum disulfide (MoS2), zinc dialkyldithophosphate (ZDDP) and MoS2/ZDDP as additives at low temperatures was investigated using an Optimol SRV reciprocating tester. Compared with MoS2 or ZDDP as single additives, the combination of MoS2 and ZDDP resulted in outstanding tribological properties, especially for higher-load, longer-duration and low-temperature working conditions. The analysis of the wear surface indicated that MoS2 not only adhered to the steel surfaces to form a solid film, but also combined with ZDDP to form a tribofilm. The active components of the additives reached the metal surfaces effectively, indicating that the polymer system did not interfere with the function of the additives. The rheological experiment results also showed that PP grease with additives can maintain stable viscoelasticity, viscosity recovery rates and ductility at low temperatures

A Framework on Analyzing Long-Term Drought Changes and Its Influential Factors Based on the PDSI

Drought is one of the most frequent and most widespread natural disasters worldwide, significantly impacting agricultural production and the ecological environment. An investigation of long-term drought changes and its influencing factors provides not only an understanding of historical droughts but also a scientific basis for the protection of future water resources. This study investigated the temporal characteristics of drought in a study site located in the center of Southwest China (SWC) over a 700-year period (AD 1300–2005) using the Palmer Drought Severity Index (PDSI). The linkage between drought and its influencing factors is discussed. An algorithm based on the random forest (RF) method was proposed to analyze the dynamic influence of the factors on drought. We also examined the linkages between the demise of two dynasties and historical drought events. The results showed that the study site was a drought-prone area in the study period and experienced a non-significant drying trend in all centuries, except for the 17th century; a total of 232 droughts were detected in the study site from AD 1300–2005. The wavelet spectrum of the PDSI series showed the existence of 4-, 8-, 16-, 32-, and 128-year-periods. A strong correlation existed between the sunspot numbers and the PDSI. The correlation of the period between the PDSI and El Niño-Southern Oscillation (ENSO) series in the same frequency domain was weak, while the ENSO exhibited a strong interaction with the PDSI in some time periods. The Pacific Decadal Oscillation (PDO) and PDSI had no resonance period in the low-frequency region, but there was a period of 80–130 years in the high-frequency region. The relative rates of influence of the ENSO, sunspot numbers, and PDO during AD 1700–1996 were 38.40%, 31.81%, and 29.8%, respectively. However, the mechanism of the interaction between droughts and the influential factors is complex, and the dominant factor changed over time. The analysis of long-term drought changes based on the PDSI series may provide clues to understand the development of historical events

Efficacy of modafinil on fatigue and excessive daytime sleepiness associated with neurological disorders: a systematic review and meta-analysis.

BACKGROUND: Modafinil is a novel wake-promoting agent approved by the FDA ameliorating excessive daytime sleepiness (EDS) in three disorders: narcolepsy, shift work sleep disorder and obstructive sleep apnea. Existing trials of modafinil for fatigue and EDS associated with neurological disorders provided inconsistent results. This meta-analysis was aimed to assess drug safety and effects of modafinil on fatigue and EDS associated with neurological disorders. METHODS: A comprehensive literature review was conducted in order to identify published studies assessing the effects of modafinil on fatigue and EDS associated with neurological disorders. Primary outcomes included fatigue and EDS. Secondary outcomes included depression and adverse effects. FINDINGS: Ten randomized controlled trials were identified including 4 studies of Parkinson's disease (PD), 3 of multiple sclerosis (MS), 2 of traumatic brain injury (TBI) and 1 of post-polio syndrome (PPS). A total of 535 patients were enrolled. Our results suggested a therapeutic effect of modafinil on fatigue in TBI (MD -0.82 95% CI -1.54 - -0.11 p=0.02, I(2)=0%), while a beneficial effect of modafinil on fatigue was not confirmed in the pooled studies of PD or MS. Treatment results demonstrated a clear beneficial effect of modafinil on EDS in patients with PD (MD -2.45 95% CI -4.00 - -0.91 p=0.002 I(2)=14%), but not with MS and TBI. No difference was seen between modafinil and placebo treatments in patients with PPS. Modafinil seemed to have no therapeutic effect on depression. Adverse events were similar between modafinil and placebo groups except that more patients were found with insomnia and nausea in modafinil group. CONCLUSIONS: Existing trials of modafinil for fatigue and EDS associated with PD, MS, TBI and PPS provided inconsistent results. The majority of the studies had small sample sizes. Modafinil is not yet sufficient to be recommended for these medical conditions until solid data are available

Bioassay-guided isolation and identification of anti-platelet-active compounds from the root of Ashitaba (<i>Angelica keiskei</i> Koidz.)

<div><p>Platelet aggregation is fundamental to a wide range of physiological and pathological processes, including the induction of thrombosis and arteriosclerosis. Anti-platelet activity of a crude methanol extract and solvent fractions of Ashitaba roots (<i>Angelica keiskei</i> Koidz.) was evaluated using a turbidimetric method using washed rabbit platelets. We identified the anti-platelet activities of two chalcones, 4-hydroxyderricin and xanthoangelol, isolated from the ethyl acetate-soluble fraction of Ashitaba roots by using a bioassay-guided isolation method. 4-Hydroxyderricin and xanthoangelol effectively inhibited platelet aggregation induced by collagen (IC₅₀ of 41.9 and 35.9 μM, respectively), platelet-activating factor (IC₅₀ of 46.1 and 42.3 μM, respectively) and phorbol 12-myristate 13-acetate (IC₅₀ of 16.5 and 45.9 μM, respectively). These compounds did not inhibit thrombin-induced platelet aggregation (IC₅₀ of>80 μM). The results suggest that the chalcones 4-hydroxyderricin and xanthoangelol may be potent anti-thrombotic components of <i>A. keiskei</i> Koidz.</p></div

Flow diagram of the literature search and study selection processes.

<p>Flow diagram of the literature search and study selection processes.</p

Effect of modafinil on study discontinuation due to adverse events.

<p>Effect of modafinil on study discontinuation due to adverse events.</p

Formation of copper boride on Cu(111)

Boron forms compounds with nearly all metals, with notable exception of copper and other group IB and IIB elements. Here, we report an unexpected discovery of ordered copper boride grown epitaxially on Cu(111) under ultrahigh vacuum. Scanning tunneling microscopy experiments combined with ab initio evolutionary structure prediction reveal a remarkably complex structure of 2D-Cu8B14. Strong intra-layer p–d hybridization and a large amount of charge transfer between Cu and B atoms are the key factors for the emergence of copper boride. This makes the discovered material unique and opens up the possibility of synthesizing ordered low-dimensional structures in similar immiscible systems

Effects of modafinil on excessive daytime sleepiness measured by Epworth Sleepiness Scale in PD (A), MS (B) and TBI (C).

<p>Effects of modafinil on excessive daytime sleepiness measured by Epworth Sleepiness Scale in PD (A), MS (B) and TBI (C).</p