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<i>CF</i><sub><i>2</i></sub><i>–II</i> Alternative Splicing Isoform Regulates the Expression of Xenobiotic Tolerance-Related Cytochrome P450 <i>CYP6CY22</i> in <i>Aphis gossypii</i> Glover
The expression of P450 genes is regulated by trans-regulatory factors or cis-regulatory
elements and
influences how endogenous or xenobiotic substances are metabolized
in an organism’s tissues. In this study, we showed that overexpression
of the cytochrome P450 gene, CYP6CY22, led to resistance
to cyantraniliprole in Aphis gossypii. The expression of CYP6CY22 increased in the midgut
and remaining carcass of the CyR strain, and after repressing the
expression of CYP6CY22, the mortality of cotton aphids
increased 2.08-fold after exposure to cyantraniliprole. Drosophila ectopically expressing CYP6CY22 exhibited tolerance
to cyantraniliprole and cross-tolerance to xanthotoxin, quercetin,
2-tridecanone, tannic acid, and nicotine. Moreover, transcription
factor CF2–II (XM_027994540.2) is transcribed only as the splicing variant isoform CF2–II-AS, which was found to be 504 nucleotides shorter than CF2–II in A. gossypii. RNAi and yeast one-hybrid (Y1H) results
indicated that CF2–II-AS positively regulates CYP6CY22 and binds to cis-acting element p (−851/-842)
of CYP6CY22 to regulate its overexpression. The above
results indicated that CYP6CY22 was regulated by
the splicing isoform CF2-II-AS, which will help us
further understand the mechanism of transcriptional adaption of cross-tolerance
between synthetic insecticides and plant secondary metabolites mediated
by P450s