2 research outputs found
Benzothiazole-Based Fluorescent Sensor for Ratiometric Detection of Zn(II) Ions and Secondary Sensing PPi and Its Applications for Biological Imaging and PPase Catalysis Assays
In
this paper, we designed and synthesized three benzothiazole-based
fluorescent probes <b>L</b><sub><b>1</b></sub>, <b>L</b><sub><b>2</b></sub>, and <b>L</b><sub><b>3</b></sub> for zinc ion detection. Among various metal ions, only the
zinc ion exhibited fluorescence enhancement at 475 nm accompanied
by the blue-shift emission wavelength in HEPES buffer solution containing
probes. Through titration experiment, the detection limit of <b>L</b><sub><b>1</b></sub> for zinc ion sensing was calculated
to be as low as 7 nM, which showed a high sensitivity. Furthermore,
the confocal laser scanning micrographs of HeLa cells demonstrate
good cell permeability of probe <b>L</b><sub><b>1</b></sub> and selective detection of zinc ion in living cells. The <b>L</b><sub><b>1</b></sub><b>–Zn</b><sup><b>2+</b></sup> complex was further used for pyrophosphate (PPi) sensing in
HEPES buffer solution, the limit of detection was calculated to be
as low as 60 nM. <b>L</b><sub><b>1</b></sub><b>–Zn</b><sup><b>2+</b></sup> can monitor the enzyme catalyzed degradation
process of PPi, thus providing a meaningful way for tracking of zinc
ion and pyrophosphate in biological systems
Visualization 1: Generation of stochastic electromagnetic beams with complete controllable coherence
Dynamic pattern with modulation magnitude 0.5 Originally published in Optics Express on 19 September 2016 (oe-24-19-21587