The expression of SRCIN1 was downregulated in the osteosarcoma cells.

<p>(A) One representive patient was diagnosed as osteosarcoma using HE staining. (B) The mRNA expression of SRCIN1 was measured by using qRT-PCR. (C) The protein expression of SRCIN1 was measured by using western blot.</p

SRCIN1 expression was reduced in the osteosarcoma tissues.

<p>(A) The mRNA expression of SRCIN1 was detected by using qRT-PCR in the osteosarcoma tissues and adjacent normal tissues. (B) SRCIN1 expression was downregulated in 29 patients (29/35, 82%) compared to the adjacent tissues. (C) The protein expression of SRCIN1 was measured by using western blot in the osteosarcoma tissues and adjacent normal tissues.</p

SRCIN1 suppressed the osteosarcoma cell proliferation.

<p>(A) The protein expression of SRCIN1 was measured by using western blot in the MG-63 cells after treated SRCIN1 vector. (B) The mRNA expression of SRCIN1 was measured by using qRT-PCR in the MG-63 cells after treated SRCIN1 vector. (C) The cell proliferation was measured by CCK-8 analysis in the MG-63 cells. (D) The mRNA expression of ki-67 was measured by using qRT-PCR. (E) The protein expression of ki-67 was measured by using western blot in the MG-63 cells after treated SRCIN1 vector.(F) The mRNA expression of PCNA was measured by using qRT-PCR. (G) The protein expression of PCNA was measured by using western blot. *p<0.05, **p<0.01 and ***p<0.001.</p

SRCIN1 repressed the osteosarcoma cell colony formation and invasion.

<p>(A) Ectopic expression of SRCIN1 inhibited the MG-63 cell colony formation. (B) Overexpression of SRCIN1 suppressed the MG-63 cell invasion. ***p<0.001.</p

SRCIN1 inhibited epithelial–mesenchymal transition of the osteosarcoma cell.

<p>(A) The mRNA expression of E-cadherin, N-cadherin, Vimentin and Snail was measured by using qRT-PCR in the MG-63 cells after treated SRCIN1 vector. (B) The protein expression of E-cadherin, N-cadherin, Vimentin and Snail was detected by using western blot in the MG-63 cells after treated SRCIN1 vector.</p

Effect of HMME-SDT on area density of collagen fibers in rabbit ear hypertrophic scars.

<p>Note: *Compared with the untreated controls P<0.01;**Compared with the lesioned P<0.05; ΔCompared with the lesioned P>0.05 ; ΔΔ Compared with the lesioned+HMME P>0.05.</p

Effect of HMME-SDT on HI of rabbit ear hypertrophic scars.

<p>Note: *Compared with the untreated controls P<0.01; **Compared with the lesioned P<0.01; ΔCompared with the lesioned P>0.05 ; ΔΔCompared with the lesioned P = 0.064; ΔΔCompared with the lesioned+HMME P>0.05.</p

The histological observation after HMME-SDT on hypertrophic scarring.

<p>A: Untreated control group HE staining×200; B: Untreated control group VG staining×200; C:Lesioned group 56 days after epithelialization HE staining×200; D:Lesioned group 56 days after epithelialization, VG staining×200 ; E:Lesioned + Treatment group 56 days after epithelialization, HE staining×200;F:Lesioned + Treatment group 56 days after epithelialization, VG staining×200</p

The genernal observation after HMME-SDT on hypertrophic scarring.

<p>United States of America A: Hypertrophic scar model; B: Scars following epithelialization for 42 days ; C: Scars following epithelialization for 56 days; D: Scars following epithelialization for 56 days in the lesioned +treatment group.</p