Electrochemical Behavior and Electrochemical Determination of Tiamulin Fumarate at an Ionic Liquid Modified Carbon Paste Electrode

The electrochemical behavior of tiamulin fumarate (TF) at ionic liquid N-Butylpyridinium hexafluorophosphate (BPPF6) modified carbon paste electrode (BPPF6/CPE) was investigated and further used for tiamulin fumarate sample determination. BPPF6/CPE showed an enhanced electrochemical response towards the electrochemical oxidation of TF. A well-defined and sensitive oxidation peak was observed at BPPF6/CPE in 0.10 M phosphate buffer solution (PBS, pH = 6.80). The oxidation peak current of TF increased significantly at BPPF6/CPE compared with that at carbon paste electrode and the BPPF6/CPE was characterized by the electrochemical impedance spectroscopy (EIS). At the same time, the electrochemical kinetics parameters of TF on the BPPF6/CPE were evaluated. Under the optimized conditions, the oxidation peak currents were linearly dependent on the concentration of TF in the range of 0.3–9.0 μM and 9.0–0.3 mM, with a detection limit of 0.16 μM (S/N = 3). The proposed method has been successfully applied in the electrochemical quantitative determination of TF content in commercial injection samples

Efficient 3PC for Binary Circuits with Application to Maliciously-Secure DNN Inference

In this work, we focus on maliciously secure 3PC for binary circuits with honest majority. While the state-of-the-art (Boyle et al. CCS 2019) has already achieved the same amortized communication as the best-known semi-honest protocol (Araki et al. CCS 2016), they suffer from a large computation overhead: when comparing with the best-known implementation result (Furukawa et al. Eurocrypt 2017) which requires $9\times$ communication cost of Araki et al., the protocol by Boyle et al. is around $4.5\times$ slower than that of Furukawa et al. In this paper, we design a maliciously secure 3PC protocol that matches the same communication as Araki et al. with comparable concrete efficiency as Furukawa et al. To obtain our result, we manage to apply the distributed zero-knowledge proofs (Boneh et al. Crypto 2019) for verifying computations over $\mathbb{Z}_2$ by using \emph{prime} fields and explore the algebraic structure of prime fields to make the computation of our protocol friendly for native CPU computation. Experiment results show that our protocol is around $3.5\times$ faster for AES circuits than Boyle et al. We also applied our protocol to the binary part (e.g. comparison and truncation) of secure deep neural network inference, and results show that we could reduce the time cost of achieving malicious security in the binary part by more than $67\%$. Besides our main contribution, we also find a hidden security issue in many of the current probabilistic truncation protocols, which may be of independent interest

The clinicopathological factors associated with disease progression in Luminal a breast cancer and characteristics of metastasis: A retrospective study from a single center in China

Background/Aim: This study investigated the clinicopathological factors associated with outcomes in patients with Luminal A breast cancer. Patients and Methods: Retrospective analysis of the association of clinicopathological factors and breast cancer outcome in 421 patients with newly diagnosed Luminal-A breast cancer that were enrolled from January 2008 to December 2014. Clinicopathological data were analyzed to validate the relationship with disease free survival (DFS) and overall survival (OS). Kaplan-Meier curves and log-rank tests were used to analyze the value of clinicopathological factors (tumor size, node status and lymphovascular invasion), and subsequent Cox regression analysis revealed significant prognostic factors. Results: With a median of 61 months follow up, the 5-year DFS and 5-year OS rate were 98.3% and 99.3%. Cox multivariate regression analysis showed that clinical anatomic stage, tumor size, status of lymph nodes, lymphovascular invasion and systemic treatment are strong prognostic factors for clinical outcome in patients with Luminal-A breast cancer. Of all 413 patients with stage I-III breast cancer, 14 presented with metastasis (3.4%) during the follow up. Bone (6/14, 42.9%) was the most common site of metastasis followed by liver (5/14, 35.7%) and lung (4/14, 28.6%). The median survival time after metastasis was 20.4 months. Of all the sites of distant metastasis, liver metastasis was the only factor that affected survival time after metastasis (χ2=6.263, p=0.012). Conclusion: Patients with Luminal A breast cancer have excellent outcomes. Liver metastasis is an important factor compressing the survival time after distant metastasis presents

Stimuli-responsive 2D polyelectrolyte photonic crystals for optically encoded pH sensing.

A versatile photonic crystal sensing motif based on a twodimensional (2D) inverse opal monolayer of stimuli-responsive polyelectrolyte gel with tunable optical properties is reported. The photonic membrane shows prompt response to pH and can be readily read out from either its optical spectra or interference colours

Genome-wide identification and expression analysis of serine proteases and homologs in the silkworm Bombyx mori

<p>Abstract</p> <p>Background</p> <p>Serine proteases (SPs) and serine proteases homologs (SPHs) are a large group of proteolytic enzymes, with important roles in a variety of physiological processes, such as cell signalling, defense and development. Genome-wide identification and expression analysis of serine proteases and their homologs in the silkworm might provide valuable information about their biological functions.</p> <p>Results</p> <p>In this study, 51 SP genes and 92 SPH genes were systematically identified in the genome of the silkworm <it>Bombyx mori</it>. Phylogenetic analysis indicated that six gene families have been amplified species-specifically in the silkworm, and the members of them showed chromosomal distribution of tandem repeats. Microarray analysis suggests that many silkworm-specific genes, such as members of SP_fam12, 13, 14 and 15, show expression patterns that are specific to tissues or developmental stages. The roles of SPs and SPHs in resisting pathogens were investigated in silkworms when they were infected by <it>Escherichia coli</it>, <it>Bacillus bombysepticus</it>, <it>Batrytis bassiana </it>and <it>B. mori </it><it>nucleopolyhedrovirus</it>, respectively. Microarray experiment and real-time quantitative RT-PCR showed that 18 SP or SPH genes were significantly up-regulated after pathogen induction, suggesting that SP and SPH genes might participate in pathogenic microorganism resistance in <it>B. mori</it>.</p> <p>Conclusion</p> <p>Silkworm SP and SPH genes were identified. Comparative genomics showed that SP and SPH genes belong to a large family, whose members are generated mainly by tandem repeat evolution. We found that silkworm has species-specific SP and SPH genes. Phylogenetic and microarray analyses provide an overview of the silkworm SP and SPHs, and facilitate future functional studies on these enzymes.</p