Additional file 2: Figure S1. of Characterization of BRCA1 and BRCA2 variants in multi-ethnic Asian cohort from a Malaysian case-control study

Association of BRCA1 and BRCA2 variants with breast cancer risk in all breast cancer cases and healthy controls. The forest plot illustrates the association of BRCA1 and BRCA2 variants with breast cancer risk in all breast cancer cases and healthy controls. Figure S2. Association of variants with breast cancer risk in ethnicity subgroups: (a) BRCA1 and (b) BRCA2. The forest plot illustrates the association of BRCA1 and BRCA2 variants with breast cancer risk in certain ethnicity subgroups that can be analyzed. (DOCX 145 kb

Additional file 1: Table S1a. of Characterization of BRCA1 and BRCA2 variants in multi-ethnic Asian cohort from a Malaysian case-control study

BRCA1 variants included in genotyping assay design. A total of 23 BRCA1 variants were included in the genotyping assay. Of these, two variants were excluded due to genotyping call rate <95%. Table S1b. BRCA2 variants included in genotyping assay design. A total of 44 BRCA2 variants were included in the genotyping assay. Of these, two variants were excluded due to genotyping call rate <95%. Table S2. Characteristics of Malaysian breast cancer cases and healthy controls in ethnicity subgroups: (a) Chinese, (b) Malay and (c) Indian. There was no difference in age for cases and controls for Chinese and Indian women, but healthy women were on average 2 years older than the cases for Malay women. Table S3a. Frequency of BRCA1 variants detected in ethnicity subgroups. The table describes the frequency of BRCA1 variants detected in Chinese, Malay and Indian women. Table S3b. Frequency of BRCA2 variants detected in ethnicity subgroups. The table describes the frequency of BRCA2 variants detected in Chinese, Malay and Indian women. (DOCX 195 kb

Patient, tumor characteristics and treatment along with the unadjusted Hazard Ratio for all cause mortality.

*<p>indicates valid proportions have been calculated (i.e., not considering unknown).</p

Survival probabilities and Hazard Ratios for all cause mortality by pN classification and LNR.

*<p>Model A is adjusted for: age, radiotherapy, surgery type, grade and tumor size and pN stage and stratified by ER Status. Model B is adjusted for: age, radiotherapy, surgery type, grade and tumor size and LNR and stratified by ER Status. Both models were internally validated using bootstrap resampling.</p

Subgroup analysis to check the added prognostic value of LNR over pN within specific subgroups.

a<p>Model adjusted for age at diagnosis, chemotherapy, radiotherapy, surgery type, grade and tumor size and stratified by ER status.</p>b<p>Model adjusted for age at diagnosis, chemotherapy, surgery type and tumor size.</p>c<p>Model adjusted for age at diagnosis, chemotherapy, radiotherapy, surgery type and tumor size and stratified by ER status. All models were internally validated using bootstrap resampling.</p

Risk reclassification table at 3 years of follow up based on models including pN stage and LNR respectively.

<p>Net Reclassification Index (NRI) = 3.2% (p value 0.08). Patients are categorized into risk categories of death based on their individual survival probabilities obtained from models A and B such that a patient with a high survival probability is categorized into the ‘low risk of death’ group and so on.</p

Cumulative overall survival by ethnicity in 5,264 South East Asian women with breast cancer.

<p>Cumulative overall survival by ethnicity in 5,264 South East Asian women with breast cancer.</p

Association between Ethnicity and All-Cause Mortality Following Diagnosis with Breast Cancer in 5,264 Southeast Asian Women.

a<p>Estimated from Cox regression model adjusted for age at diagnosis, center, and year of diagnosis.</p>b<p>Estimated from Cox regression model adjusted for age at diagnosis, center, year of diagnosis, tumor size, lymph node involvement, distant metastasis, estrogen receptor status, progesterone receptor status, and tumor grade.</p>c<p>Estimated from Cox regression model adjusted for age at diagnosis, center, year of diagnosis, tumor size, lymph node involvement, distant metastasis, estrogen receptor status, progesterone receptor status, tumor grade, loco-regional therapy, chemotherapy, and hormone therapy.</p

Distribution of Patient Profile, Tumor Characteristics and Treatment According to Ethnicity in 5,264 Southeast Asian Women with Breast Cancer.

a<p>Column percentage is presented except for center where row percentage is presented.</p>b<p>Compared using χ² test for categorical variables and Kruskal Wallis test for continuous variables.</p>c<p>Absolute tumor size was only available in 4 359 patients i.e. in 80.4% of the Chinese, 89.2% of Malays and 88.5% of Indians.</p>d<p>Only includes 4,589 patients with TNM stage I to stage III breast cancer. Complete treatment consists of mastectomy, or breast conserving surgery followed by radiotherapy. Incomplete treatment includes breast conserving surgery only or radiotherapy only.</p>e<p>Only includes 2 772 patients with estrogen or progesterone receptor positive tumors.</p

Kaplan Meier survival curves of low, intermediate and high-risk groups.

<p>Risk groups were defined by tertiles of risk scores of prediction models for patients with <i>de novo</i> metastatic breast cancer.</p