Solid-Supported Porphyrins Useful for the Synthesis of Conjugates with Oligomeric Biomolecules

<i>meso</i>-Tris­(pyridin-4-yl)­(4-carboxyphenyl)­porphyrin and 2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-a (Photochlor, HPPH) were amide-coupled to 1<i>R</i>,2<i>S</i>,3<i>R</i>,4<i>R</i>-2,3-dihydroxy-4-(hydromethyl)-1-aminocyclopentane and immobilized via an ester linkage to long chain alkyl amine-derivatized controlled pore glass (LCAA-CPG). The applicability of these supports (<b>5</b> and <b>6</b>) for the synthesis of porphyrin conjugates with oligomeric biomolecules was demonstrated using an automated phosphoramidite coupling chemistry. Cleavage from the support with concentrated ammonia gave the products, viz., porphyrin conjugates of oligonucleotides (<b>7</b>–<b>9</b>) and dendritic glycoclusters (<b>10</b>–<b>13</b>) and a cyclooctyne derivative (<b>14</b>) in 23–58% yield. In addition, the synthesized cyclooctyne derivative of <i>meso</i>-tris­(pyridin-4-yl)­(4-carboxyphenyl)­porphyrin (<b>14</b>) was conjugated with an azidopropyl-modified hyaluronic acid (<b>19</b>). The hyaluronic acid–porphyrin conjugate (<b>15</b>) was radiolabeled with ⁶⁴Cu and its (<b>15</b>[⁶⁴Cu]) receptor binding affinity to CD44-expressing tumor cells was evaluated