45,769 research outputs found
Growth- and substrate-dependent transcription of formate dehydrogenase and hydrogenase coding genes in Syntrophobacter fumaroxidans and Methanospirillum hungatei
Transcription of genes coding for formate dehydrogenases (fdh genes) and hydrogenases (hyd genes) in Syntrophobacter fumaroxidans and Methanospirillum hungatei was studied following growth under different conditions. Under all conditions tested, all fdh and hyd genes were transcribed. However, transcription levels of the individual genes varied depending on the substrate and growth conditions. Our results strongly suggest that in syntrophically grown S. fumaroxidans cells, the [FeFe]-hydrogenase (encoded by Sfum_844-46), FDH1 (Sfum_2703-06) and Hox (Sfum_2713-16) may confurcate electrons from NADH and ferredoxin to protons and carbon dioxide to produce hydrogen and formate, respectively. Based on bioinformatic analysis, a membrane-integrated energy-converting [NiFe]-hydrogenase (Mhun_1741-46) of M. hungatei might be involved in the energy-dependent reduction of CO2 to formylmethanofuran. The best candidates for F420-dependent N5,N10-methyl-H4 MPT and N5,N10,-methylene-H4MPT reduction are the cytoplasmic [NiFe]-hydrogenase and FDH1. 16S rRNA ratios indicate that in one of the triplicate co-cultures of S. fumaroxidans and M. hungatei, less energy was available for S. fumaroxidans. This led to enhanced transcription of genes coding for the Rnf-complex (Sfum_2694-99) and of several fdh and hyd genes. The Rnf-complex probably reoxidized NADH with ferredoxin reduction, followed by ferredoxin oxidation by the induced formate dehydrogenases and hydrogenase
Bridgeness: A Local Index on Edge Significance in Maintaining Global Connectivity
Edges in a network can be divided into two kinds according to their different
roles: some enhance the locality like the ones inside a cluster while others
contribute to the global connectivity like the ones connecting two clusters. A
recent study by Onnela et al uncovered the weak ties effects in mobile
communication. In this article, we provide complementary results on document
networks, that is, the edges connecting less similar nodes in content are more
significant in maintaining the global connectivity. We propose an index named
bridgeness to quantify the edge significance in maintaining connectivity, which
only depends on local information of network topology. We compare the
bridgeness with content similarity and some other structural indices according
to an edge percolation process. Experimental results on document networks show
that the bridgeness outperforms content similarity in characterizing the edge
significance. Furthermore, extensive numerical results on disparate networks
indicate that the bridgeness is also better than some well-known indices on
edge significance, including the Jaccard coefficient, degree product and
betweenness centrality.Comment: 10 pages, 4 figures, 1 tabl
Polypyrrole-coated fabric strain sensor with high sensitivity and good stability
Author name used in this publication: Hing Yee J. TsangAuthor name used in this publication: C. W. M. YuenVersion of RecordPublishe
Near-Optimal Distributed Approximation of Minimum-Weight Connected Dominating Set
This paper presents a near-optimal distributed approximation algorithm for
the minimum-weight connected dominating set (MCDS) problem. The presented
algorithm finds an approximation in rounds,
where is the network diameter and is the number of nodes.
MCDS is a classical NP-hard problem and the achieved approximation factor
is known to be optimal up to a constant factor, unless P=NP.
Furthermore, the round complexity is known to be
optimal modulo logarithmic factors (for any approximation), following [Das
Sarma et al.---STOC'11].Comment: An extended abstract version of this result appears in the
proceedings of 41st International Colloquium on Automata, Languages, and
Programming (ICALP 2014
Nucleon spin-flavor structure in SU(3) breaking chiral quark model
The SU(3) symmetric chiral quark model, which describes interactions between
quarks, gluons and the Goldstone bosons, explains reasonably well many aspects
of the flavor and spin structure of the proton, except for the values of
and . Introducing the SU(3)-breaking effect
suggested by the mass difference between the strange and nonstrange quarks, we
find that this discrepancy can be removed and better overall agreement
obtained.Comment: 18 pages, Latex, 4 tables. Phys. Rev. D (in press, submitted/revised
in June/Nov 1996
Parsec-scale jet properties of the gamma-ray quasar 3C 286
The quasar 3C~286 is one of two compact steep spectrum sources detected by
the {\it Fermi}/LAT. Here, we investigate the radio properties of the
parsec(pc)-scale jet and its (possible) association with the -ray
emission in 3C~286. The Very Long Baseline Interferometry (VLBI) images at
various frequencies reveal a one-sided core--jet structure extending to the
southwest at a projected distance of 1 kpc. The component at the jet base
showing an inverted spectrum is identified as the core, with a mean brightness
temperature of ~K. The jet bends at about 600 pc (in
projection) away from the core, from a position angle of to
. Based on the available VLBI data, we inferred the proper motion
speed of the inner jet as mas yr (), corresponding to a jet speed of about at an inclination
angle of between the jet and the line of sight of the observer. The
brightness temperature, jet speed and Lorentz factor are much lower than those
of -ray-emitting blazars, implying that the pc-scale jet in 3C~286 is
mildly relativistic. Unlike blazars in which -ray emission is in
general thought to originate from the beamed innermost jet, the location and
mechanism of -ray emission in 3C~286 may be different as indicated by
the current radio data. Multi-band spectrum fitting may offer a complementary
diagnostic clue of the -ray production mechanism in this source.Comment: 9 pages, 4 figures, accept for publication in MNRA
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