Elektrokemijski imunosenzor za dokazivanje progesterona u mlijeku krava muzara

In this study, an electrochemical immunosensor for milk progesterone produced by dairy cows was developed. Using the immunosensor, milk progesterone levels in healthy estrus dairy cows was found to range from 1 to 6 ng/mL 20 days after estrus. There were high levels of progesterone in the milk from cows with prolonged luteal phase and luteal cysts, which ranged from 15 to 28 and 19 to 29 ng/mL, respectively. Cows with inactive ovaries also showed low milk progesterone levels of 1-8 ng/mL, but they had lower plasma follicle-stimulating hormone (FSH) levels than the healthy estrous cycle dairy cows.Ovim je istraživanjem razvijen elektrokemijski imunosenzor za otkrivanje progesterona u mlijeku dobivenom od mliječnih krava. Upotrebom imunosenzora u zdravih krava za proizvodnju mlijeka 20 dana nakon estrusa utvrđene su razine progesterona u rasponu od 1 do 6 ng/mL. U mlijeku krava s produljenom luteinskom fazom i luteinskim cistama utvrđena je visoka razina progesterona, u rasponu od 15 do 28 te od 19 do 29 ng/mL. Krave s neaktivnim jajnicima također su pokazale nisku razinu progesterona u mlijeku, od 1 do 8 ng/mL. Te su krave, u odnosu na zdrave krave za proizvodnju mlijeka u estrusnoj fazi ciklusa, očitovale nižu razinu folikulostimulacijskog hormona u plazmi (FSH)

Asymptomatic ratio for seasonal H1N1 influenza infection among schoolchildren in Taiwan

Studies indicate that asymptomatic infections do indeed occur frequently for both seasonal and pandemic influenza, accounting for about one-third of influenza infections. Studies carried out during the 2009 pH1N1 pandemic have found significant antibody response against seasonal H1N1 and H3N2 vaccine strains in schoolchildren receiving only pandemic H1N1 monovalent vaccine, yet reported either no symptoms or only mild symptoms

Dysglycemia but not lipids is associated with abnormal urinary albumin excretion in diabetic kidney disease: a report from the Kidney Early Evaluation Program (KEEP)

BACKGROUND: The relationship between glycemic control and lipid abnormalities with urinary albumin-creatinine ratio (ACR) in chronic kidney disease (CKD) patients with diabetes mellitus (DM) is unknown. We sought to investigate the association of dyslipidemia and glycemic control with levels of albuminuria in the National Kidney Foundation (NKF) Kidney Early Evaluation Program (KEEP) participants with DM and CKD stage 3 or higher. METHODS: We performed a cross-sectional study of 6639 eligible KEEP patients with DM and CKD Stage 3 to 5 from June 2008 to December 2009. Multivariate logistic regression was used to evaluate the association of lipid parameters (per 10 mg/dl change in serum level) and glycosylated hemoglobin (HbA1c) values with three degrees of albuminuria normo (<30 mg⁄g), micro (30 to 300 mg⁄g) and macro (>300 mg⁄g). RESULTS: 2141 KEEP participants were included. HbA1c levels were strongly associated with micro-albuminuria (compared to normo-albuminuria) and macro-albuminuria (compared to normo-albuminuria and micro-albuminuria). Each 1.0% increase in HbA1c increased the odds of micro-albuminuria by 32% (OR 1.32, 95% CI 1.23-1.42) and the odds of macro-albuminuria (vs. microalbuminuria) by 16% (OR 1.16, 95% CI 1.05-1.28). Only increases in serum HDL were associated with decreased odds of micro-albuminuria; otherwise, the association between other components of the serum lipid profile with urinary ACR did not reach statistical significance. CONCLUSION: In this cross-sectional study of 2141 KEEP participants with DM and CKD stages 3–5, overall glycemic control but not lipids were associated with abnormal urinary albumin excretion, a marker of increased risk for progressive disease

Monitoring of Bright Blazars with MAGIC in the 2007/2008 Season

Because of the short duty-cycles and observation-time constraints, studies of bright TeV (E>100 GeV) blazars are mostly restricted to flaring episodes or rather short (days to few weeks) multiwavelength campaigns. At the same time, long-term studies of these objects are essential to gain a more complete understanding of the blazar phenomenon and to constrain theoretical models concerning jet physics. Only unbiased long-term studies are adequate for the determination of flaring state probabilities and for estimating the statistical significance of possible correlations between TeV flaring states and other wavebands or observables, such as neutrino events. Regular observations also provide triggers for multiwavelength ToO observations originating from the TeV waveband. These are particularly needed to identify and study orphan TeV flares, i.e. flares without counterparts in other wavebands. In 2007/8 the MAGIC telescope has monitored three TeV blazars on a regular basis: Mrk 501, Mrk 421, and 1ES 1959+650. We present preliminary results of these observations including the measured light curves and a correlation study for VHE gamma-rays and X-rays and VHE gamma-rays and optical R-band for Mrk 421.Comment: 10 pages, 7 figures, Proceedings of the SciNEGHE'0

Vitamin D status in youth with type 1 and type 2 diabetes enrolled in the Pediatric Diabetes Consortium (PDC) is not worse than in youth without diabetes

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/134500/1/pedi12340.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/134500/2/pedi12340_am.pd

A cross‐sectional view of the current state of treatment of youth with type 2 diabetes in the USA: enrollment data from the Pediatric Diabetes Consortium Type 2 Diabetes Registry

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/136377/1/pedi12377_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136377/2/pedi12377.pd

Limits on Light Weakly Interacting Massive Particles from the First 102.8 kg ×{\times} day Data of the CDEX-10 Experiment

We report the first results of a light weakly interacting massive particles (WIMPs) search from the CDEX-10 experiment with a 10 kg germanium detector array immersed in liquid nitrogen at the China Jinping Underground Laboratory with a physics data size of 102.8 kg day. At an analysis threshold of 160 eVee, improved limits of 8 $\times 10^{-42}$ and 3 $\times 10^{-36}$ cm$^{2}$ at a 90\% confidence level on spin-independent and spin-dependent WIMP-nucleon cross sections, respectively, at a WIMP mass ($m_{\chi}$) of 5 GeV/${c}^2$ are achieved. The lower reach of $m_{\chi}$ is extended to 2 GeV/${c}^2$.Comment: 5 pages, 4 figure

Malaria and other vector-borne infection surveillance in the U.S. Department of Defense Armed Forces Health Surveillance Center-Global Emerging Infections Surveillance program: review of 2009 accomplishments

Vector-borne infections (VBI) are defined as infectious diseases transmitted by the bite or mechanical transfer of arthropod vectors. They constitute a significant proportion of the global infectious disease burden. United States (U.S.) Department of Defense (DoD) personnel are especially vulnerable to VBIs due to occupational contact with arthropod vectors, immunological naiveté to previously unencountered pathogens, and limited diagnostic and treatment options available in the austere and unstable environments sometimes associated with military operations. In addition to the risk uniquely encountered by military populations, other factors have driven the worldwide emergence of VBIs. Unprecedented levels of global travel, tourism and trade, and blurred lines of demarcation between zoonotic VBI reservoirs and human populations increase vector exposure. Urban growth in previously undeveloped regions and perturbations in global weather patterns also contribute to the rise of VBIs. The Armed Forces Health Surveillance Center-Global Emerging Infections Surveillance and Response System (AFHSC-GEIS) and its partners at DoD overseas laboratories form a network to better characterize the nature, emergence and growth of VBIs globally. In 2009 the network tested 19,730 specimens from 25 sites for Plasmodium species and malaria drug resistance phenotypes and nearly another 10,000 samples to determine the etiologies of non-Plasmodium species VBIs from regions spanning from Oceania to Africa, South America, and northeast, south and Southeast Asia. This review describes recent VBI-related epidemiological studies conducted by AFHSC-GEIS partner laboratories within the OCONUS DoD laboratory network emphasizing their impact on human populations