224 research outputs found
Effect of size, shape, and surface modification on cytotoxicity of gold nanoparticles to human Hep-2 and canine MDCK cells
There have been increasing interests in applying gold nanoparticles in biological research, drug delivery, and therapy. As the interaction of gold nanoparticles with cells relies on properties of nanoparticles, the cytotoxicity is complex and still under debating. In this work, we investigate the cytotoxicity of gold nanoparticles of different encapsulations, surface charge states, sizes and shapes to both human HEp-2 and canine MDCK cells. We found that cetyltrimethylammonium-bromide- (CTAB-) encapsulated gold nanorods (GNRs) were relatively higher cytotoxic than GNRs undergone further polymer coating and citrate stabilized gold nanospheres (GNSs). The toxicity of CTAB-encapsulated GNRs was mainly caused by CTAB on GNRs’ surface but not free CTAB in the solution. No obvious difference was found among GNRs of different aspect ratios. Time-lapse study revealed that cell death caused by GNRs occurred predominately within one hour through apoptosis, whereas cell death by free CTAB was in a time- and dose-dependent manner. Both positively and negatively surface-charged polymer-coated GNRs (PSS-GNRs and PAH-PSS-GNRs) showed similar levels of cytotoxic, suggesting the significance of surface functionality rather than surface charge in this case
Effect of size, shape, and surface modification on cytotoxicity of gold nanoparticles to human Hep-2 and canine MDCK cells
There have been increasing interests in applying gold nanoparticles in biological research, drug delivery, and therapy. As the interaction of gold nanoparticles with cells relies on properties of nanoparticles, the cytotoxicity is complex and still under debating. In this work, we investigate the cytotoxicity of gold nanoparticles of different encapsulations, surface charge states, sizes and shapes to both human HEp-2 and canine MDCK cells. We found that cetyltrimethylammonium-bromide- (CTAB-) encapsulated gold nanorods (GNRs) were relatively higher cytotoxic than GNRs undergone further polymer coating and citrate stabilized gold nanospheres (GNSs). The toxicity of CTAB-encapsulated GNRs was mainly caused by CTAB on GNRs’ surface but not free CTAB in the solution. No obvious difference was found among GNRs of different aspect ratios. Time-lapse study revealed that cell death caused by GNRs occurred predominately within one hour through apoptosis, whereas cell death by free CTAB was in a time- and dose-dependent manner. Both positively and negatively surface-charged polymer-coated GNRs (PSS-GNRs and PAH-PSS-GNRs) showed similar levels of cytotoxic, suggesting the significance of surface functionality rather than surface charge in this case
Effect of size, shape, and surface modification on cytotoxicity of gold nanoparticles to human Hep-2 and canine MDCK cells
There have been increasing interests in applying gold nanoparticles in biological research, drug delivery, and therapy. As the interaction of gold nanoparticles with cells relies on properties of nanoparticles, the cytotoxicity is complex and still under debating. In this work, we investigate the cytotoxicity of gold nanoparticles of different encapsulations, surface charge states, sizes and shapes to both human HEp-2 and canine MDCK cells. We found that cetyltrimethylammonium-bromide- (CTAB-) encapsulated gold nanorods (GNRs) were relatively higher cytotoxic than GNRs undergone further polymer coating and citrate stabilized gold nanospheres (GNSs). The toxicity of CTAB-encapsulated GNRs was mainly caused by CTAB on GNRs’ surface but not free CTAB in the solution. No obvious difference was found among GNRs of different aspect ratios. Time-lapse study revealed that cell death caused by GNRs occurred predominately within one hour through apoptosis, whereas cell death by free CTAB was in a time- and dose-dependent manner. Both positively and negatively surface-charged polymer-coated GNRs (PSS-GNRs and PAH-PSS-GNRs) showed similar levels of cytotoxic, suggesting the significance of surface functionality rather than surface charge in this case
Effect of Baicalin on inflammatory mediator levels and microcirculation disturbance in rats with severe acute pancreatitis
Objective: To investigate the effect of Bacailin on inflammatory mediator levels and microcirculation disturbance in severe acute pancreatitis (SAP) rats and explore its therapeutic mechanism on this disease. Methods: SAP model rats were randomly divided into model control group and Baicalin treated group, 45 rats in each group. The same number of normal rats were included in sham-operated group. These groups were further subdivided into 3 h, 6 h and 12 h subgroups, respectively (15 rats in each subgroup). At 3, 6 and 12 hours after operation, rats were killed to conduct the following experiments: (1) to examine the mortality rates of rats, the ascites volume and pancreatic pathological changes in each group; (2) to determine the contents of amylase, PLA~2~, TXB~2~, PGE~2~, PAF and IL-1[beta]; in blood as well as the changes in blood viscosity.Results: (1) Compared to model control group, treatment with Baicalin is able to improve the pathological damage of the pancreas, lower the contents of amylase and multiple inflammatory mediators in blood, decrease the amount of ascitic fluid and reduce the mortality rates of SAP rats; (2) at 3 hours after operation, the low-shear whole blood viscosity in Baicalin treated group was significantly lower than that in model control group;at 12 hours after operation, both the high-shear and low-shear whole blood viscosity in Baicalin treated group were also significantly lower than those in model control group.Conclusion: Baicalin, as a new drug, has good prospects in the treatment of SAP since it can exert therapeutic effects on this disease through inhibiting the production of inflammatory mediators, lowering blood viscosity, improving microcirculation and mitigating the pathological damage of the pancreas
Long-term Orbital Period Variation of Hot Jupiters from Transiting Time Analysis using TESS Survey Data
Many hot Jupiters may experience orbital decays, which are manifested as
long-term transit timing variations. We have analyzed 7068 transits from the
Transiting Exoplanet Survey Satellite (TESS) for a sample of 326 hot Jupiters.
These new mid-transit time data allow us to update ephemerides for these
systems. By combining the new TESS transit timing data with archival data, we
search for possible long-term orbital period variations in these hot Jupiters
using a linear and a quadratic ephemeris model. We identified 26 candidates
that exhibit possible long-term orbital period variations, including 18
candidates with decreasing orbital periods and 8 candidates with increasing
orbital periods. Among them, 12 candidates have failed in our leave-one-out
cross-validation (LOOCV) test and thus should be considered as marginal
candidates. In addition to tidal interaction, alternative mechanisms such as
apsidal precession, R{\o}mer effect, and Applegate effect could also contribute
to the observed period variations. The ephemerides derived in this work are
useful for scheduling follow-up observations for these hot Jupiters in the
future. The Python code used to generate the ephemerides is made available
online.Comment: Accepted for publication in ApJ
Effect of size, shape, and surface modification on cytotoxicity of gold nanoparticles to human HEp-2 and canine MDCK cells
There have been increasing interests in applying gold nanoparticles in biological research, drug delivery, and therapy. As the interaction of gold nanoparticles with cells relies on properties of nanoparticles, the cytotoxicity is complex and still under debating. In this work, we investigate the cytotoxicity of gold nanoparticles of different encapsulations, surface charge states, sizes and shapes to both human HEp-2 and canine MDCK cells. We found that cetyltrimethylammonium-bromide-(CTAB-) encapsulated gold nanorods (GNRs) were relatively higher cytotoxic than GNRs undergone further polymer coating and citrate stabilized gold nanospheres (GNSs). The toxicity of CTAB-encapsulated GNRs was mainly caused by CTAB on GNRs' surface but not free CTAB in the solution. No obvious difference was found among GNRs of different aspect ratios. Time-lapse study revealed that cell death caused by GNRs occurred predominately within one hour through apoptosis, whereas cell death by free CTAB was in a time-and dose-dependent manner. Both positively and negatively surface-charged polymer-coated GNRs (PSS-GNRs and PAH-PSS-GNRs) showed similar levels of cytotoxic, suggesting the significance of surface functionality rather than surface charge in this case
Simulation of thermal stress and fatigue life prediction of high speed steel work roll during hot rolling considering the initial residual stress
Considerable residual stress is produced during heat treatment. Compressive residual stress at the shell is conductive to improving the thermal fatigue life of a work roll, while tensile stress in the core could cause thermal breakage. In hot rolling, thermal stress occurs under the heating-cooling cycles over the roll surface due to the contact with the hot strip and water spray cooling. The combination of thermal stress and residual stress remarkably influences the life of a work roll. In this paper, finite element method (FEM) simulation of hot rolling is performed by treating the residual stress as the initial stress. Afterwards, the effects of the initial roll temperature and cooling conditions on thermal stress considering the initial residual stress are discussed. Lastly, the thermal fatigue life of a work roll is estimated based on the strain life model. The higher initial roll temperature causes a higher temperature but a lower compressive thermal stress at the roll surface. The surface temperature and compressive stress increase significantly in the insufficient cooling conditions, as well as the center tensile stress. The calculation of the fatigue life of a work roll based on the universal slopes model according to the 10% rule and 20% rule is reasonable compared with experimental results
High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors
Recombinant vectors based on a non-pathogenic human parvovirus, the adeno-associated virus 2 (AAV2) have been developed, and are currently in use in a number of gene therapy clinical trials. More recently, a number of additional AAV serotypes have also been isolated, which have been shown to exhibit selective tissue-tropism in various small and large animal models1. Of the 10 most commonly used AAV serotypes, AAV3 is by far the least efficient in transducing cells and tissues in vitro as well as in vivo
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