Electrospun Thymosin Beta-4 Loaded PLGA/PLA Nanofiber/ Microfiber Hybrid Yarns for Tendon Tissue Engineering Application

Microfiber yarns (MY) have been widely employed to construct tendon tissue grafts. However, suboptimal ultrastructure and inappropriate environments for cell interactions limit their clinical application. Herein, we designed a modified electrospinning device to coat poly(lactic-co-glycolic acid) PLGA nanofibers onto polylactic acid (PLA) MY to generate PLGA/PLA hybrid yarns (HY), which had a well-aligned nanofibrous structure, resembling the ultrastructure of native tendon tissues and showed enhanced failure load compared to PLA MY. PLGA/PLA HY significantly improved the growth, proliferation, and tendon-specific gene expressions of human adipose derived mesenchymal stem cells (HADMSC) compared to PLA MY. Moreover, thymosin beta-4 (Tβ4) loaded PLGA/PLA HY presented a sustained drug release manner for 28 days and showed an additive effect on promoting HADMSC migration, proliferation, and tenogenic differentiation. Collectively, the combination of Tβ4 with the nano-topography of PLGA/PLA HY might be an efficient strategy to promote tenogenesis of adult stem cells for tendon tissue engineering

Periodically driven four-dimensional topological insulator with tunable second Chern number

In recent years, Floquet engineering has attracted considerable attention as a promising approach for tuning topological phase transitions. In this work, we investigate the effects of high-frequency time-periodic driving in a four-dimensional (4D) topological insulator, focusing on topological phase transitions at the off-resonant quasienergy gap. The 4D topological insulator hosts gapless three-dimensional boundary states characterized by the second Chern number $C_{2}$. We demonstrate that the second Chern number of 4D topological insulators can be modulated by tuning the amplitude of time-periodic driving. This includes transitions from a topological phase with $C_{2}=\pm3$ to another topological phase with $C_{2}=\pm1$, or to a topological phase with an even second Chern number $C_{2}=\pm2$ which is absent in the 4D static system. Finally, the approximation theory in the high-frequency limit further confirms the numerical conclusions.Comment: 11 pages, 9 figures. arXiv admin note: text overlap with arXiv:2312.1601

Optical transitions between Landau levels: AA-stacked bilayer graphene

The low-frequency optical excitations of AA-stacked bilayer graphene are investigated by the tight-binding model. Two groups of asymmetric LLs lead to two kinds of absorption peaks resulting from only intragroup excitations. Each absorption peak obeys a single selection rule similar to that of monolayer graphene. The excitation channel of each peak is changed as the field strength approaches a critical strength. This alteration of the excitation channel is strongly related to the setting of the Fermi level. The peculiar optical properties can be attributed to the characteristics of the LL wave functions of the two LL groups. A detailed comparison of optical properties between AA-stacked and AB-stacked bilayer graphenes is also offered. The compared results demonstrate that the optical properties are strongly dominated by the stacking symmetry. Furthermore, the presented results may be used to discriminate AABG from MG, which can be hardly done by STM