Improving the Orbits of the BDS-2 IGSO and MEO Satellites with Compensating Thermal Radiation Pressure Parameters

The orbit accuracy of the navigation satellites relies on the accurate knowledge of the forces on the spacecraft, in particular the non-conservative perturbations. This study focuses on the Inclined Geosynchronous Orbit (IGSO) and Medium Earth Orbit (MEO) satellites of the regional Chinese BeiDou Navigation Satellite System (BDS-2), for which apparent deficiencies of non-conservative models are identified and evidenced in the Satellite Laser Ranging (SLR) residuals. The orbit errors derived from the empirical 5-parameter Extended CODE Orbit Model (ECOM) as well as a semi-analytical adjustable box-wing model show prominent dependency on the Sun elongation angle, even in the yaw-steering attitude mode. Hence, a periodic acceleration in the normal direction of the +X surface, presumably generated by the mismodeled thermal radiation pressure, is introduced. The SLR validations reveal that the Sun elongation angle-dependent systematic errors were significantly reduced, and the orbit accuracy was improved by 10–30% to approximately 4.5 cm and 3.0 cm for the BDS-2 IGSO and MEO satellites, respectively

Structural and molecular basis of ZNRF3/RNF43 transmembrane ubiquitin ligase inhibition by the Wnt agonist R-spondin

The four R-spondin (Rspo) proteins are secreted agonists of Wnt signalling in vertebrates, functioning in embryogenesis and adult stem cell biology. Through ubiquitination and degradation of Wnt receptors, the transmembrane E3 ubiquitin ligase ZNRF3 and related RNF43 antagonize Wnt signalling. Rspo ligands have been reported to inhibit the ligase activity through direct interaction with ZNRF3 and RNF43. Here we report multiple crystal structures of the ZNRF3 ectodomain (ZNRF3ecto), a signalling-competent Furin1–Furin2 (Fu1–Fu2) fragment of Rspo2 (Rspo2Fu1–Fu2), and Rspo2Fu1–Fu2 in complex with ZNRF3ecto, or RNF43ecto. A prominent loop in Fu1 clamps into equivalent grooves in the ZNRF3ecto and RNF43ecto surface. Rspo binding enhances dimerization of ZNRF3ecto but not of RNF43ecto. Comparison of the four Rspo proteins, mutants and chimeras in biophysical and cellular assays shows that their signalling potency depends on their ability to recruit ZNRF3 or RNF43 via Fu1 into a complex with LGR receptors, which interact with Rspo via Fu2

Advancing the Solar Radiation Pressure Model for BeiDou-3 IGSO Satellites

In the absence of detailed surface information, empirical solar radiation pressure (SRP) models, such as the five-parameter Empirical CODE Orbit Model (ECOM1) and its extended version-ECOM2, are widely used for modeling SRP forces acting on GNSS satellites. This study shows that the orbits of BeiDou-3 Inclined Geosynchronous Orbit satellites (IGSOs) determined with the ECOM1 model suffer from systematic once-per-revolution radial orbit errors, which can be partly reduced by the ECOM2 model. To eliminate such orbit errors, the BeiDou-3 IGSO optical coefficients are solved by using an adjustable box-wing (ABW) model and then introduced into an a priori box-wing SRP model to enhance the ECOM1 model (ECOM1 + BW). In the ABW solution, in addition to satellite body and solar panels, the contributions of the communication payloads installed on BeiDou-3 IGSO ±X panels on the SRP are also considered, which markedly improves the stability of the optical coefficient estimates. The efficiency of the developed a priori box-wing model is demonstrated through eliminated once-per-revolution radial orbit errors and decreased day boundary discontinuities. However, the orbit solutions still show significant degradations during eclipse seasons. The results of the first yaw-attitude analysis for eclipsing BeiDou-3 IGSOs show that their yaw behaviors are the same as those of BeiDou-3 CAST (China Academy of Space Technology) MEOs (Medium Earth Orbit satellites), and have been well considered in the study. This rules out the possibility that attitude errors are the potential reason for the orbit deterioration. By introducing a once-per-revolution sine term in the Sun direction (Ds term) and keeping Ds active during the Earth’s shadow transitions to the ECOM1 + BW model, the orbit performance inside the eclipse seasons is significantly improved and can be comparable to that outside the eclipse seasons

Lysine-Rich Extracellular Rings Formed by hβ2 Subunits Confer the Outward Rectification of BK Channels

The auxiliary β subunits of large-conductance Ca2+-activated K+ (BK) channels greatly contribute to the diversity of BK (mSlo1 α) channels, which is fundamental to the adequate function in many tissues. Here we describe a functional element of the extracellular segment of hβ2 auxiliary subunits that acts as the positively charged rings to modify the BK channel conductance. Four consecutive lysines of the hβ2 extracellular loop, which reside sufficiently close to the extracellular entryway of the pore, constitute three positively charged rings. These rings can decrease the extracellular K+ concentration and prevent the Charybdotoxin (ChTX) from approaching the extracellular entrance of channels through electrostatic mechanism, leading to the reduction of K+ inflow or the outward rectification of BK channels. Our results demonstrate that the lysine rings formed by the hβ2 auxiliary subunits influences the inward current of BK channels, providing a mechanism by which current can be rapidly diminished during cellular repolarization. Furthermore, this study will be helpful to understand the functional diversity of BK channels contributed by different auxiliary β subunits

Tirofiban for Stroke without Large or Medium-Sized Vessel Occlusion

The effects of the glycoprotein IIb/IIIa receptor inhibitor tirofiban in patients with acute ischemic stroke but who have no evidence of complete occlusion of large or medium-sized vessels have not been extensively studied. In a multicenter trial in China, we enrolled patients with ischemic stroke without occlusion of large or medium-sized vessels and with a National Institutes of Health Stroke Scale score of 5 or more and at least one moderately to severely weak limb. Eligible patients had any of four clinical presentations: ineligible for thrombolysis or thrombectomy and within 24 hours after the patient was last known to be well; progression of stroke symptoms 24 to 96 hours after onset; early neurologic deterioration after thrombolysis; or thrombolysis with no improvement at 4 to 24 hours. Patients were assigned to receive intravenous tirofiban (plus oral placebo) or oral aspirin (100 mg per day, plus intravenous placebo) for 2 days; all patients then received oral aspirin until day 90. The primary efficacy end point was an excellent outcome, defined as a score of 0 or 1 on the modified Rankin scale (range, 0 [no symptoms] to 6 [death]) at 90 days. Secondary end points included functional independence at 90 days and a quality-of-life score. The primary safety end points were death and symptomatic intracranial hemorrhage. A total of 606 patients were assigned to the tirofiban group and 571 to the aspirin group. Most patients had small infarctions that were presumed to be atherosclerotic. The percentage of patients with a score of 0 or 1 on the modified Rankin scale at 90 days was 29.1% with tirofiban and 22.2% with aspirin (adjusted risk ratio, 1.26; 95% confidence interval, 1.04 to 1.53, P = 0.02). Results for secondary end points were generally not consistent with the results of the primary analysis. Mortality was similar in the two groups. The incidence of symptomatic intracranial hemorrhage was 1.0% in the tirofiban group and 0% in the aspirin group. In this trial involving heterogeneous groups of patients with stroke of recent onset or progression of stroke symptoms and nonoccluded large and medium-sized cerebral vessels, intravenous tirofiban was associated with a greater likelihood of an excellent outcome than low-dose aspirin. Incidences of intracranial hemorrhages were low but slightly higher with tirofiban

Study on a High-frequency Multi-GNSS Real-time Precise Clock Estimation Algorithm and Application in GNSS Augment System

GNSS satellite-based differential augment system is based on real-time orbit and clock augment message. The multi-GNSS real-time precise clock error estimation model is studied, and then the parameters estimated in traditional un-difference model are optimized and a high-efficient real-time clock simplified model is proposed and realized. The real-time orbit data processing based on PANDA is also analyzed. The results indicate that the real-time orbit radial accuracy of GPS, BeiDou MEO and Galileo is 1~5 cm, and the radial accuracy of the BeiDou GEO/IGSO satellite is about 10 cm. It is found that the optimized real-time clock simplified model is more efficient in one epoch than un-difference model and can be applied to high-frequency (such as 1 Hz) updating of real-time clock augment message. The results show that the real-time clock error obtained by this model is absolute value and there is no constant bias. Based on the real-time orbit, the GPS real-time clock precision of the simplified model is about 0.24 ns, BeiDou GEO is about 0.50 ns, IGSO/MEO is about 0.22 ns and Galileo is about 0.32 ns. Using the multi-GNSS real-time data stream in GFZ, a multi-GNSS real-time augment prototype system is built and the real-time augment message is being broadcasted on the Internet. The real-time PPP centimeter-level service and meter-level navigation service based on pseudorange are realized based on this prototype system

Improving the Orbits of the BDS-2 IGSO and MEO Satellites with Compensating Thermal Radiation Pressure Parameters

The orbit accuracy of the navigation satellites relies on the accurate knowledge of the forces on the spacecraft, in particular the non-conservative perturbations. This study focuses on the Inclined Geosynchronous Orbit (IGSO) and Medium Earth Orbit (MEO) satellites of the regional Chinese BeiDou Navigation Satellite System (BDS-2), for which apparent deficiencies of non-conservative models are identified and evidenced in the Satellite Laser Ranging (SLR) residuals. The orbit errors derived from the empirical 5-parameter Extended CODE Orbit Model (ECOM) as well as a semi-analytical adjustable box-wing model show prominent dependency on the Sun elongation angle, even in the yaw-steering attitude mode. Hence, a periodic acceleration in the normal direction of the +X surface, presumably generated by the mismodeled thermal radiation pressure, is introduced. The SLR validations reveal that the Sun elongation angle-dependent systematic errors were significantly reduced, and the orbit accuracy was improved by 10–30% to approximately 4.5 cm and 3.0 cm for the BDS-2 IGSO and MEO satellites, respectively