Onlone 00:00

Being alone is not the only definition of loneliness. Loneliness can be felt even when surrounded by a lot of people, especially in the virtual online world. Our digital devices play an important role in connecting everyone together without the restriction of time and space. Communication became more and more convenient in this era. Mostly we are digitally connected, but sometimes, we are mentally disconnected. We are online and together in this virtual world, but loneliness is always a never ended situation that we are suffering from. As a visual communicator, My works focus on using performance as an approach to explore the evolving relationship between the online communication and online loneliness. In my thesis research, I want to investigate how does the online world created more loneliness to individuals digitally and physically, and how people release their spiritual desire and overcome loneliness in the online world

A Novel System for Rapid and Cost-Effective Production of Detection and Diagnostic Reagents of West Nile Virus in Plants

The threat of West Nile virus (WNV) epidemics necessitates the development of a technology platform that can produce reagents to support detection and diagnosis rapidly and inexpensively. A plant expression system is attractive for protein production due to its low-cost and high-scalability nature and its ability to make appropriate posttranslational modifications. Here, we investigated the feasibility of using plants to produce two WNV detection and diagnostic reagents to address the current cost and scalability issues. We demonstrated that WNV DIII antigen and E16 monoclonal antibody are rapidly produced at high levels in two plant species and are easily purified. Furthermore, they are effective in identifying WNV and in detecting human IgM response to WNV infection. E16 mAb does not cross-react with other flaviviruses, therefore, is valuable for improving diagnostic accuracy. This study provides a proof of principle for using plants as a robust and economical system to produce diagnostic reagents for arboviruses

Motivation for users’ knowledge sharing behavior in virtual brand communities : A psychological ownership perspective

The project is supported by National Social Science Foundation of China (21BGL132), National Natural Science Foundation of China (NSFC) (71802097), Jinan University Management School Funding Program (GY21013), Institute for Enterprise Development, Jinan University, Guangdong Province (2021MYZD04, HS2102 and 2020CP03), Philosophy and Social Sciences Planning Program of Guangzhou (2021GZYB05) and Research Institute on Brand Innovation and Development of Guangzhou (2021CS05). The first two authors contributed equally to this work.Peer reviewe

Antibody-Dependent Enhancement Activity of a Plant-Made Vaccine Against West Nile Virus

West Nile virus (WNV) causes annual outbreaks globally and is the leading cause of mosquito-borne disease in Unite States. In the absence of licensed therapeutics, there is an urgent need to develop effective and safe human vaccines against WNV. One of the major safety concerns for WNV vaccine development is the risk of increasing infection by related flaviviruses in vaccinated subjects via antibody-dependent enhancement of infection (ADE). Herein, we report the development of a plant-based vaccine candidate that provides protective immunity against a lethal WNV challenge mice, while minimizes the risk of ADE for infection by Zika (ZIKV) and dengue (DENV) virus. Specifically, a plant-produced virus-like particle (VLP) that displays the WNV Envelope protein domain III (wDIII) elicited both high neutralizing antibody titers and antigen-specific cellular immune responses in mice. Passive transfer of serum from VLP-vaccinated mice protected recipient mice from a lethal challenge of WNV infection. Notably, VLP-induced antibodies did not enhance the infection of Fc gamma receptor-expressing K562 cells by ZIKV or DENV through ADE. Thus, a plant-made wDIII-displaying VLP presents a promising WNV vaccine candidate that induces protective immunity and minimizes the concern of inducing ADE-prone antibodies to predispose vaccinees to severe infection by DENV or ZIKV

Vibration Response Characteristics and Application of Existing Railway Subgrade

The existing conventional methods of subgrade disease assessment are not suitable for the existing lines. There are many research studies on the vibration response and attenuation law of the railway subgrade, but few research studies focus on the vibration response and attenuation law caused by the weak subgrade. In this study, vibration response tests were carried out at different positions and depths of the subgrade before and after reinforcement improvement. The results show that vibration response near the ballast is obvious, and it attenuates with the increase of the horizontal distance from the rail; the vibration acceleration response of the subgrade after reinforcement changes greatly; the vibration response curve of the reinforced section is spindle shaped, and the vertical vibration acceleration response attenuates obviously at the depth of 6.5 m, only about 10% to 30% of the surface; the vibration acceleration of the subgrade with reinforcement at the depth of 4.5 m attenuates to 60% of the surface; the vibration acceleration of the subgrade without reinforcement at the depth of 4.5 m attenuates to 50%–60% of the surface

Plant-Produced Antigen Displaying Virus-Like Particles Evokes Potent Antibody Responses against West Nile Virus in Mice

In this study, we developed a hepatitis B core antigen (HBcAg)-based virus-like particle (VLP) that displays the West Nile virus (WNV) Envelope protein domain III (wDIII) as a vaccine candidate for WNV. The HBcAg-wDIII fusion protein was quickly produced in Nicotiana benthamiana plants and reached a high expression level of approximately 1.2 mg of fusion protein per gram of leaf fresh weight within six days post gene infiltration. Electron microscopy and gradient centrifugation analysis indicated that the introduction of wDIII did not interfere with VLP formation and HBcAg-wDIII successfully assembled into VLPs. HBcAg-wDIII VLPs can be easily purified in large quantities from Nicotiana benthamiana leaves to >95% homogeneity. Further analysis revealed that the wDIII was displayed properly and demonstrated specific binding to an anti-wDIII monoclonal antibody that recognizes a conformational epitope of wDIII. Notably, HBcAg-wDIII VLPs were shown to be highly immunogenic and elicited potent humoral responses in mice with antigen-specific IgG titers equivalent to that of protective wDIII antigens in previous studies. Thus, our wDIII-based VLP vaccine offers an attractive option for developing effective, safe, and low-cost vaccines against WNV

Fundamental study of ductile-regime diamond turning of single crystal gallium arsenide

Gallium arsenide (GaAs) components, ranging from the planar substrate to those possessing complicated shapes and microstructures, have attracted extensive interest regarding their applications in photovoltaic devices, photodetectors and emerging quantum devices. Single point diamond turning (SPDT) is regarded as an excellent candidate for an industrially viable mechanical machining process, as it can generate nano-smooth surfaces, even on some hard-to-machine brittle materials such as silicon and silicon carbide, with a single pass. However, the extremely low fracture toughness and strong anisotropic machinability of GaAs makes it difficult to obtain nano-smooth, crack-free machined surfaces. To bridge the current knowledge gaps in understanding the anisotropic machinability of GaAs, this paper studied the mechanical material properties of (001)-oriented GaAs through indentation tests, assuming the diagonals of the indenter acted in the similar way of the cutting edge of a diamond tool with a negative rake angle. The results showed that the (001) plane of the GaAs material displayed harder and more brittle when indented along direction I (one diagonal of indenter parallel to the orientation) compared to direction II (one diagonal of indenter parallel to the orientation), which coincides with anisotropic machined surface quality by SPDT. This finding reveals, for the first time, that the crystallographic orientation dependence of both hardness and fracture toughness represents the underlying mechanism for the anisotropic machinability of GaAs. The paper presents a novel approach to evaluate the critical depth of cut under a high cutting speed comparable to SPDT and to determine the maximum feed rate for ductile-regime diamond turning. The 26.57 nm critical depth of cut was obtained for the hardest cutting direction using a large negative rake angle diamond tool. Finally, a nano-smooth surface was successfully generated along all the orientations in ductile-regime diamond turning, in which the material removal mechanism is considered as plastic deformation caused by high-density dislocations. The subsurface layer remains to its single crystal structure and no cracks are found under a transmission electron microscope (TEM). The results proves the proposed evaluation approach for the critical depth of cut and the maximum allowed feed rate is highly effective for guiding the ductile-regime machining of brittle materials

Understanding local consumers' reactions to perceived unfair product recalls of foreign brands : a relative deprivation perspective

Peer reviewedPostprin