Field-applied fungicides and postharvest treatments to control asparagus diseases posing biosecurity threats to New Zealand

Collaborative research between New Zealand and Australia has investigated field-applied fungicides and postharvest treatments for control of asparagus rust (caused by Puccinia asparagi) and phomopsis stem blight of asparagus (caused by Phomopsis asparagi) in Queensland. In a 2004 field trial, the fungicides difenoconazole and propiconazole reduced the incidence of asparagus rust. In a 2005 field trial, four fungicides (carbendazim, chlorothalonil, iprodione and propiconazole) reduced the severity of phomopsis stem blight. Postharvest disinfection with sodium hypochlorite or with calcium hypochlorite at 150 ppm was highly effective at reducing Pu. asparagi and Ph. asparagi spore germination on water agar. Integrated disease management based on field and postharvest methods are suggested to reduce the risk of importation of the diseases to New Zealand. Similar methods could be used for controlling these diseases if they establish

Plastron osteotomy in the management of fishing hook ingestion in a Malayan box turtle (Cuora amboinensis)

An adult male Malayan box turtle was diagnosed with foreign body obstruction of fishing hook. Upon presentation, the animal had pale mucous membrane and fishing line protruding out from the oral cavity. Diagnosis of foreign body ingestion was based on plain radiograph where the fishing hook was evidenced on the lateral and ventrodorsal radiographic view. Plastron osteotomy surgical removal was performed after endoscopy guided forcep removal failed. Procedures were carried out under the injectable anesthesia using a combination of ketamine and xylazine hydrochloride. Antibiotic, anti-inflammatory and parenteral fluid therapy was given pre and post-surgically as medical treatment and stabilisation

Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR

Rattus argentiventer (Rice Field Rat) is responsible for destruction of paddy. The mammal is known for its rapid reproductive potential which can be a target for biological control of this species. Phospholipase C-zeta (PLC ζ) is a specific enzyme found in sperm of mammals responsible for triggering calcium oscillations leading to egg activation during fertilization. It facilitates the first step of egg activation causing egg division and subsequent development into an embryo. The method used in this study was to identify PLC gene fragments from the testis of Rattus argentiventer using two-step Reverse Transcriptase Polymerase Chain Reaction (PCR) and quantified with Real Time Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR). Following that, sequencing of PLC was performed. The identified sequence was then keyed in into the BLAST portal in NCBI (National Centre of Bioinformatics) for comparison with a standard PLC sequence from Rattus norvegicus for sequence alignment. The result showed that PLCζ was present in Rattus argentiventer and qRTPCR could quantify the amount of PLCζ available.As such this would give additional information of detection and quantification techniques for rapid identification and detection of PLCζ to be carried out in the approach of controlling the rapid growth of Rattus argentiventer population

ENGINEERING CONCEPTS IN ANALYSING LUMBOSACRAL LOAD IN POST-OPERATIVE SCOLIOTIC PATIENTS

Lumbosacral alignment plays a major role in the mechanical low back pain in normal population. Malalignment causes increased strain to the muscles and ligaments around it which eventually leads to mechanical low back pain and discomfort. The level of strain a ligament receives in the lumbosacral junction depends upon the lumbosacral angle and the load exerted on it. If there is an easy way to find the load exerted at the lumbosacral junction, then it would be trouble free for the physicians to examine. Hence, the treatment can be planned accordingly. This study analysis the angle, net force produced and stress faced by the ligaments around the lumbosacral joint in postoperative scoliosis patients using radiographic images. Study design was analytical, observational cross sectional study. Radiographs of 30 patients were collected and one sample ‘t ’test was used for data analysis with ‘p ’value set as 0.05 as level of significance .The mean lumbosacral angle of the postoperative scoliotic patients was found to be 45.56 degrees and the standard deviation was ± 8.7156. The results suggest that patients who underwent scoliosis correction surgery did not have an optimal lumbosacral angle of 30 degrees

Dataset on gene expression in the elderly after Mindfulness Awareness Practice or Health Education Program

It has been reported that relaxation techniques can improve physical health and cognitive function. A number of studies involving different types of relaxation practices showed changes in expression of genes. We investigated the gene expression pattern of a cohort of elderly subjects of Asian descent after weekly (for the first three months) and monthly (for the subsequent six months) intervention. Sixty consenting elderly subjects (aged 60–90 years) with mild cognitive impairment were assigned to either the Mindfulness Awareness Practice (MAP) or Health Education Program (HEP) group in a randomized controlled trial to assess the effectiveness of the programs in preventing further cognitive decline and evaluate the influence on neurological, cellular and biochemical factors. Blood samples were collected before the start of intervention and after nine months for gene expression profiling using Affymetrix Human Genome U133 Plus 2.0 arrays. The dataset is publicly available for further analyses

Influence of layer thickness to the emission spectra in microcavity organic light emitting diodes

Microcavity organic light emitting diodes (OLEDs) have attracted great attention because they can reduce the width of emission spectra from organic materials, enhance brightness and achieve multipeak emission from the same material. In this work, we have fabricated microcavity OLEDs with widely used organic materials, such as N,N'-di(naphthalene-1-yl)-N,N'-diphenylbenzidine (NPB) as a hole transport layer and tris (8-hydroxyquinoline) (Alq) as emitting and electron transporting layer. These organic materials are sandwiched either between two thick silver mirrors or one thin copper and one thick silver mirrors. The influence of total cavity length (from 164 nm to 243nm) and the cavity Q-factor to the emission behavior has been investigated. In all cases, an OLED without bottom mirror, i.e. with the organic materials sandwiched between indium tin oxide and a thick silver mirror, has been fabricated for comparison. We have characterized the devices with photoluminescence, electroluminescence, and reflectance measurements. Multiple peaks have been observed for some devices at larger viewing angles

Identification of the genomic mutation in Epha4rb-2J/rb-2J mice

The EphA4 receptor tyrosine kinase is involved in numerous cell-signalling activities during embryonic development. EphA4 has the ability to bind to both types of ephrin ligands, the ephrinAs and ephrinBs. The C57BL/6J-Epha4rb-2J/GrsrJ strain, denoted Epha4rb-2J/rb-2J, is a spontaneous mouse mutant that arose at The Jackson Laboratory. These mutants exhibited a synchronous hind limb locomotion defect or “hopping gait” phenotype, which is also characteristic of EphA4 null mice. Genetic complementation experiments suggested that Epha4rb-2J corresponds to an allele of EphA4, but details of the genomic defect in this mouse mutant are currently unavailable. We found a single base-pair deletion in exon 9 resulting in a frame shift mutation that subsequently resulted in a premature stop codon. Analysis of the predicted structure of the truncated protein suggests that both the kinase and sterile α motif (SAM) domains are absent. Definitive determination of genotype is needed for experimental studies of mice carrying the Epha4rb-2J allele, and we have also developed a method to ease detection of the mutation through RFLP. Eph-ephrin family members are reportedly expressed as numerous isoforms. Hence, delineation of the specific mutation in EphA4 in this strain is important for further functional studies, such as protein–protein interactions, immunostaining and gene compensatory studies, investigating the mechanism underlying the effects of altered function of Eph family of receptor tyrosine kinases on phenotype