Isolation of Candida species in children and their biofilm-forming ability on nano-composite surfaces

Candida species including Candida albicans, Candida krusei and Candida glabrata are opportunistic microorganisms that inhabit oral cavity. The objective of this study is to determine the effect of dental caries on Candida spp. biofilm-forming ability on nano-composite with the hypothesis that dental caries enhances the colonization of Candida spp. To assess Candida spp. colonisation in the oral cavity of the paediatric patient, samples were obtained from 30 subjects aged five to six years old from Kuantan, Pahang, Malaysia. The samples were collected from buccal mucosa, palate and tooth surfaces using sterile swabs. 10 mL of patient’s saliva suspension was also collected. Following that, the samples were inoculated on CHROMagar and incubated for 24 h at 37 ºC. Candida biofilm of caries isolate C. albicans (HNFC2), and C. albicans ATCC 32354 were developed on three different types of nano-composites. The study showed that no C. albicans was isolated from the caries-free oral cavity while 76% of children with caries possessed Candida spp. 65% of the yeasts were isolated from the tooth surface. Only 35% of the total isolates were obtained from soft tissues, including palatal and buccal mucosa. C. albicans is the most isolated Candida spp. with 82% and 67% of the yeast were obtained from the tooth surface and buccal mucosa, respectively. Besides, HNFC2 significantly colonised the nano-composites more than the ATCC (P < 0.05). In the comparison of the three types of nano-composites, nano-hybrid-based containing pre-polymerised filler (cB) exhibited the least C. albicans HNFC2 cells colonisation with 7.7 x 10³ cells mL-1. In contrast, the nano-composite that contained bulk-filled nanohybrid (cC) was the most colonised with 14.3 x 10³ cells mL-1. In conclusion, dental caries enhances the colonization of Candida spp. in children's oral cavity, and that caries isolate form more biofilm on nano-composites compared to the lab strain C. albicans

The effect of dental caries on the colonization of Candida species: an in vitro study

Introduction: Candida species are opportunistic microorganisms that inhabit oral cavity. The species of Candida that are normally isolated from the oral cavity include Candida albicans, Candida krusei and Candida glabrata. The objective of this study is to determine the effect of caries towards colonization of Candida spp. with the hypothesis that dental caries enhance the colonization of Candida spp. Materials and methods: To assess the colonization of Candida spp. in the oral cavity of paediatric patient, samples were obtained from 30 subjects aged five to six years old from International Islamic University Malaysia (IIUM) Dental Clinic and Adik Arif Kindergarten in Kuantan, Pahang, Malaysia. The samples were collected from buccal mucosa, palate and tooth surfaces using sterile swab. In addition, 10 mL of patient’s saliva suspension was also collected. Following that, the samples were inoculated aseptically on CHROMagar and incubated for 24 h to 48 h at 37 ºC. Finally, the colour, morphology and colony forming unit were determined. Results: This study has shown that no C. albicans was isolated from caries-free oral cavity. Meanwhile, 76% of children with caries possessed Candida spp. in their oral cavity with 65% of Candida spp. isolated was from the tooth surface. Only 35% of the total isolates were obtained from soft tissues including palatal and buccal mucosa. In addition, 82% and 67% of the yeast isolated from the tooth surface and buccal mucosa, respectively were C. albicans. Conclusion: In conclusion, dental caries enhances the colonization of Candida spp. in children’s oral cavity

The effect of dental caries in children on the colonisation of Candida species

Objective: To determine the effect of caries towards colonization of Candida spp. Methods: To assess the colonization of Candida spp. in the oral cavity of paediatric patient, samples were obtained from 30 subjects aged five to six years old from International Islamic University Malaysia (IIUM) Dental Clinic and Adik Arif Kindergarten in Kuantan, Pahang, Malaysia. The samples were collected from buccal mucosa, palate and tooth surfaces using sterile swab. In addition, 10 mL of patient’s saliva suspension was also collected. Following that, the samples were inoculated aseptically on CHROMagar and incubated for 24 h to 48 h at 37 ºC. Finally, the colour, morphology and colony forming unit were determined. Results: This study has shown that no C. albicans was isolated from caries-free oral cavity. Meanwhile, 76% of children with caries possessed Candida spp. in their oral cavity with 65% of Candida spp. isolated was from the tooth surface. Only 35% of the total isolates were obtained from soft tissues including palatal and buccal mucosa. In addition, 82% and 67% of the yeast isolated from the tooth surface and buccal mucosa, respectively were C. albicans. Meanwhile, only C. albicans (1.2 0.5 x 105 cfu mL-1) was isolated from saliva of children with caries. Conclusions: Dental caries enhance the colonization of Candida spp. particularly C. albicans in children’s oral cavity