46 research outputs found
Pharmaceutical Development and Safety Evaluation of a GMP-Grade Fucoidan for Molecular Diagnosis of Cardiovascular Diseases
The adhesion molecule P-selectin is present on the cell surface of both activated endothelium and activated platelets. The present study describes the pharmaceutical development, safety evaluation, and preclinical efficacy of a micro-dosed radiotracer. The macromolecular nanoscale assembly consisted of a natural compound made of a sulfated fucose-rich polysaccharides (fucoidan) and a radionuclide (technetium-99m) for the detection of P-selectin expression in cardiovascular diseases. After extraction and fractionation from brown seaweeds, the good manufacturing practice (GMP) production of a low molecular weight (LMW) fucoidan of 7 kDa was achieved and full physicochemical characterization was performed. The regulatory toxicology study in rats of the GMP batch of LMW fucoidan revealed no adverse effects up to 400 μg/kg (×500 higher than the expected human dose) and pseudoallergy was not seen as well. In a myocardial ischemia-reperfusion model in rats, the GMP-grade LMW fucoidan labeled with technetium-99m detected P-selectin upregulation in vivo. The present study supports the potential of using 99mTc-fucoidan as an imaging agent to detect activated endothelium in humans
The European project NanoAthero to fight cardiovascular diseases using nanotechnologies
International audienc
Nanoparticules à coeur de poly(cyanoacrylate d'alkyle) et couronne de polysaccharides obtenues par polymérisation radicalaire rédox en émulsion dans l'eau
CHATENAY M.-PARIS 11-BU Pharma. (920192101) / SudocPARIS-BIUP (751062107) / SudocSudocFranceF
Radical Emulsion Polymerization of Alkylcyanoacrylates Initiated by the Redox System DextranâCerium(IV) under Acidic Aqueous Conditions
International audienc
A new approach for the characterization of insoluble amphiphilic copolymers based on their emulsifying properties
International audienc
Novel Polysaccharide-Decorated Poly(Isobutyl Cyanoacrylate) Nanoparticles
International audienc
Plug-in spectrometry with optical fibers as a novel analytical tool for nanoparticles technology: Application to the investigation of the emulsion polymerization of the alkylcyanoacrylate
International audienc
Polysaccharide Nanosystems for Future Progress in Cardiovascular Pathologies
International audienc
Radical Emulsion Polymerization of Alkylcyanoacrylates Initiated by the Redox System DextranâCerium(IV) under Acidic Aqueous Conditions
Nanomedicine for the molecular diagnosis of cardiovascular pathologies
International audienc