3 research outputs found
Asymmetric dimethylarginineβnature, analytical methods for determination, and clinical applications
Πsymmetric dimethylarginine (ADMA) is a major endogenous and competitive inhibitor of nitric oxide (NO) synthesis. Reduced production and/or bioavailability of NO is considered the central mechanism responsible for endothelial dysfunction (ED) that precedes atherosclerosis manifestation. Πsymmetric dimethylarginine is a promising biomarker and a risk factor for cardiovascular desease (CVD). Elevated plasma concentrations of ADMA are associated with numerous CVD and metabolic diseases. This study aimed to review the current knowledge about biology and clinical implication of ADMA as a new class biomarker. A summary analysis of currently validated analytical methods for the determination of serum and plasma ADMA was performed
Laboratory Cardiovascular Risk Assessment in Individuals with Long-Standing Type I Diabetes Mellitus β Adipokines, Osteoprotegerin, Asymmetric Dimethyl-Arginine // ΠΠ°Π±ΠΎΡΠ°ΡΠΎΡΠ½Π° ΠΎΡΠ΅Π½ΠΊΠ° Π½Π° ΡΡΡΠ΄Π΅ΡΠ½ΠΎ-ΡΡΠ΄ΠΎΠ² ΡΠΈΡΠΊ ΠΏΡΠΈ Π»ΠΈΡΠ° Ρ Π΄ΡΠ»Π³ΠΎΠ³ΠΎΠ΄ΠΈΡΠ΅Π½ Π·Π°Ρ Π°ΡΠ΅Π½ Π΄ΠΈΠ°Π±Π΅Ρ ΡΠΈΠΏ I - Π°Π΄ΠΈΠΏΠΎΠΊΠΈΠ½ΠΈ, ΠΎΡΡΠ΅ΠΎΠΏΡΠΎΡΠ΅Π³Π΅ΡΠΈΠ½ ΠΈ Π°ΡΠΈΠΌΠ΅ΡΡΠΈΡΠ΅Π½ Π΄ΠΈΠΌΠ΅ΡΠΈΠ»-Π°ΡΠ³ΠΈΠ½ΠΈΠ½
This thesis focuses on studying diabetes mellitus type 1 (T1D) as a present-day disease with socially significant implications and laboratory cardiovascular risk (CVR) assessment in providing comprehensive patient care. With increased life expectancy, cardiovascular diseases (CVD) are becoming the main cause of mortality with T1D. To date, risk stratification and screening strategies for assessing CVR in T1D have been primarily extrapolated from studies of patients with T2D or the total population. Laboratory evaluation of CVR by introducing innovative biomarkers would allow optimisation of diagnostic and prognostic accuracy and therapeutic approaches. This thesis aims to analyse the prognostic value of: asymmetric dimethyl-arginine (ADMA), osteoprotegerin (OPG), adiponectin (ADNC) and leptin (Lep) against specific CVR assessment tools - ST1RE and ESC-2019. The prognostic value of ADMA, OPG, ADNC, Lep and blood count parameters were evaluated against a constructed model β a combination of established in clinical practice RFs for the development of CVD in DM (RiskFactor 3: HbA1C β₯ 7%, CRP β₯ 3 mg/l and AlbU β₯ 30 mg/l). Cut-off values were established and can be applied in routine practice. The present study also analysed the interactions between heamatomorphologic parameters and proposed biomarkers in the context of high CVR in individuals with long-standing T1D.ΠΠΈΡΠ΅ΡΡΠ°ΡΠΈΠΎΠ½Π½ΠΈΡΡ ΡΡΡΠ΄ Π΅ ΠΏΠΎΡΠ²Π΅ΡΠ΅Π½ Π½Π° Π°ΠΊΡΡΠ°Π»Π½ΠΎ ΡΠΎΡΠΈΠ°Π»Π½ΠΎ-Π·Π½Π°ΡΠΈΠΌΠΎ Π·Π°Π±ΠΎΠ»ΡΠ²Π°Π½Π΅ β Π·Π°Ρ
Π°ΡΠ΅Π½ Π΄ΠΈΠ°Π±Π΅Ρ ΡΠΈΠΏ 1 (Π’1Π) ΠΈ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½Π° ΠΎΡΠ΅Π½ΠΊΠ° Π½Π° ΡΡΡΠ΄Π΅ΡΠ½ΠΎ-ΡΡΠ΄ΠΎΠ² ΡΠΈΡΠΊ (Π‘Π‘Π ) Π² ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½Π°ΡΠ° Π³ΡΠΈΠΆΠ° Π·Π° Π±ΠΎΠ»Π½ΠΈΡ. Π‘ ΡΠ²Π΅Π»ΠΈΡΠ°Π²Π°Π½Π΅ Π½Π° ΠΏΡΠΎΠ΄ΡΠ»ΠΆΠΈΡΠ΅Π»Π½ΠΎΡΡΡΠ° Π½Π° ΠΆΠΈΠ²ΠΎΡ, ΡΡΡΠ΄Π΅ΡΠ½ΠΎ-ΡΡΠ΄ΠΎΠ²ΠΈΡΠ΅ Π·Π°Π±ΠΎΠ»ΡΠ²Π°Π½ΠΈΡ (Π‘Π‘Π) ΡΠ΅ ΠΏΡΠ΅Π²ΡΡΡΠ°Ρ Π² ΠΎΡΠ½ΠΎΠ²Π½Π° ΠΏΡΠΈΡΠΈΠ½Π° Π·Π° ΡΠΌΡΡΡΠ½ΠΎΡΡ ΠΏΡΠΈ Π’1Π. ΠΡΠΌ Π΄Π½Π΅ΡΠ½Π° Π΄Π°ΡΠ° ΡΡΡΠ°ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡΡΠ° Π½Π° ΡΠΈΡΠΊΠ° ΠΈ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³ΠΎΠ²ΠΈΡΠ΅ ΡΡΡΠ°ΡΠ΅Π³ΠΈΠΈ Π·Π° ΠΎΡΠ΅Π½ΠΊΠ° Π½Π° Π‘Π‘Π ΠΏΡΠΈ Π’1Π ΡΠ° ΠΏΡΠ΅Π΄ΠΈΠΌΠ½ΠΎ Π΅ΠΊΡΡΠ°ΠΏΠΎΠ»ΠΈΡΠ°Π½ΠΈ ΠΎΡ ΠΏΡΠΎΡΡΠ²Π°Π½ΠΈΡ ΠΏΡΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΈ Ρ Π’2Π ΠΈΠ»ΠΈ ΠΎΠ±ΡΠ°ΡΠ° ΠΏΠΎΠΏΡΠ»Π°ΡΠΈΡ. ΠΠ°Π±ΠΎΡΠ°ΡΠΎΡΠ½Π°ΡΠ° ΠΎΡΠ΅Π½ΠΊΠ° Π½Π° Π‘Π‘Π ΠΏΠΎΡΡΠ΅Π΄ΡΡΠ²ΠΎΠΌ Π²ΡΠ²Π΅ΠΆΠ΄Π°Π½Π΅ΡΠΎ Π½Π° ΠΈΠ½ΠΎΠ²Π°ΡΠΈΠ²Π½ΠΈ Π±ΠΈΠΎΠΌΠ°ΡΠΊΠ΅ΡΠΈ Π±ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»Π° ΠΎΠΏΡΠΈΠΌΠΈΠ·ΠΈΡΠ°Π½Π΅ Π½Π° Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ½ΠΎ-ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ½Π°ΡΠ° Π°ΠΊΡΡΠ°ΡΠ½ΠΎΡΡ ΠΈ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ½ΠΈΡΠ΅ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΠΈ. Π¦Π΅Π»ΡΠ° Π½Π° Π΄ΠΈΡΠ΅ΡΡΠ°ΡΠΈΠΎΠ½Π½ΠΈΡΡ ΡΡΡΠ΄ Π΅ Π΄Π° ΡΠ΅ Π°Π½Π°Π»ΠΈΠ·ΠΈΡΠ° ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ½Π° ΡΡΠΎΠΉΠ½ΠΎΡΡ Π½Π°: Π°ΡΠΈΠΌΠ΅ΡΡΠΈΡΠ΅Π½ Π΄ΠΈΠΌΠ΅ΡΠΈΠ»Π°ΡΠ³ΠΈΠ½ΠΈΠ½ (ΠDMA), ΠΎΡΡΠ΅ΠΎΠΏΡΠΎΡΠ΅Π³Π΅ΡΠΈΠ½ (OPG), Π°Π΄ΠΈΠΏΠΎΠ½Π΅ΠΊΡΠΈΠ½ (ADNC) ΠΈ Π»Π΅ΠΏΡΠΈΠ½ (Lep) ΡΠΏΡΡΠΌΠΎ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΈ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½ΡΠΈ Π·Π° ΠΎΡΠ΅Π½ΠΊΠ° Π½Π° Π‘Π‘Π - ST1RE ΠΈ ESC ΠΎΡ 2019 Π³. ΠΡΠ΅Π½Π΅Π½Π° Π΅ ΠΈ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ½Π° ΡΡΠΎΠΉΠ½ΠΎΡΡ Π½Π° ADMA, OPG, ADNC ΠΈ Lep ΡΠΏΡΡΠΌΠΎ ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΡ ΠΎΡ ΡΡΠ²ΡΡΠ΄Π΅Π½ΠΈ Π² ΠΊΠ»ΠΈΠ½ΠΈΡΠ½Π°ΡΠ° ΠΏΡΠ°ΠΊΡΠΈΠΊΠ° ΡΠΈΡΠΊΠΎΠ²ΠΈ ΡΠ°ΠΊΡΠΎΡΠΈ Π·Π° ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ Π½Π° Π‘Π‘Π ΠΏΡΠΈ Π·Π°Ρ
Π°ΡΠ΅Π½ Π΄ΠΈΠ°Π±Π΅Ρ (RiskFactor3: HbA1C β₯7%, CRP β₯3 mg/l ΠΈ AlbU β₯30 mg/l). ΠΠ·Π²Π΅Π΄Π΅Π½ΠΈ ΡΠ° cut-off ΡΡΠΎΠΉΠ½ΠΎΡΡΠΈ, ΠΊΠΎΠΈΡΠΎ ΠΌΠΎΠ³Π°Ρ Π΄Π° Π±ΡΠ΄Π°Ρ ΠΏΡΠΈΠ»ΠΎΠΆΠ΅Π½ΠΈ Π² ΡΡΡΠΈΠ½Π½Π°ΡΠ° ΠΏΡΠ°ΠΊΡΠΈΠΊΠ°. Π Π½Π°ΡΡΠΎΡΡΠΎΡΠΎ ΠΏΡΠΎΡΡΠ²Π°Π½Π΅ ΡΠ΅ Π°Π½Π°Π»ΠΈΠ·ΠΈΡΠ°Π½ΠΈ ΠΈ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡΡΠ° ΠΌΠ΅ΠΆΠ΄Ρ Ρ
Π΅ΠΌΠ°ΡΠΎΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΠΈ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΠΈ ΠΏΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½ΠΈΡΠ΅ Π±ΠΈΠΎΠΌΠ°ΡΠΊΠ΅ΡΠΈ Π² ΠΊΠΎΠ½ΡΠ΅ΠΊΡΡΠ° Π½Π° Π²ΠΈΡΠΎΠΊ Π‘Π‘Π ΠΏΡΠΈ Π»ΠΈΡΠ° Ρ Π΄ΡΠ»Π³ΠΎΠ³ΠΎΠ΄ΠΈΡΠ΅Π½ Π’1Π
The role of education and its link to metabolic control in patients with type 1 diabetes mellitus with long duration
Type 1 diabetes mellitus (T1DM) is a disease with constantly increasing incidence. Acquiring knowledge and diabetes skills is an invariable and important part of the disease treatment during all life cycles. The association between socioeconomic status and, in particular, the level of educational qualification and the metabolic control in patients with long-duration of T1DM is not enough studied. The results received in the current study show that there is, although weak, association between the level of education and the glycaemic control, the presence of dyslipidaemia, and obesity in patients with T1DM with long duration