EVALUATION OF ANTICANCER ACTIVITY OF ETHANOLIC EXTRACT OF CYPERUS KYLLINGIA ENDL. IN EHRLICH ASCITES CARCINOMA-INDUCED SWISS ALBINO MICE

Objective: The purpose of the study was to evaluate the antitumor and antioxidant status of ethanolic extract of Cyperus kyllingia Endl. on Ehrlich ascites carcinoma (EAC)-treated mice.Methods: The determination of in vivo antitumor activity was performed using EAC cells inoculated mice groups (n=12). The groups were treated for 9 consecutive days with ethanolic extract of C. kyllingia (EECK) at the doses of 20 and 40 mg/kg b.w., respectively. After 24 h of the last dose, half of the mice were sacrificed and the rest were kept alive for assessment of increase in life span. The antitumor potential of EECK was assessed by evaluating tumor volume, viable and non-viable tumor cell count, tumor weight, hematological parameters, and biochemical estimations. Furthermore, antioxidant parameters were assayed by estimating liver tissue enzymes.Results: EECK showed direct cytotoxicity on EAC cell line in a dose-dependent manner. EECK exhibited significant (p<0.05) decrease in the tumor volume, viable cell count, tumor weight, and elevated the life span of EAC tumor-bearing mice. The hematological profile, biochemical estimations, and tissue antioxidant assay were reverted to normal level in EECK-treated mice.Conclusion: Experimental results revealed that EECK possesses potent antitumor and antioxidant properties. Further, research is going on to find out the active principle(s) of EECK for better understanding of mechanism of its antitumor and antioxidant activity

Phytochemical investigation and exploration of CNS depressant efficacy of ethanolic root extract of <em>Cyperus kyllinga </em> Endl.

801-804Sedative drugs such as diazepam, lorazepam, pentoberbitone, amphetamine derivatives etc., are known to have permanent side effects. However, addiction to such drugs, possibly out of depression, is not uncommon. Similar drugs from plant origin with same efficacy could be a better safe alternative. In this study, we evaluated ethanolic extract of roots of the Cyperus kyllinga Endl. (Nut grass) for central nervous system (CNS) depressant activity (sedative activity) at the dose 20 and 40 mg/kg body wt. when administered i.p. The standard drug used was diazepam. The CNS activity was assayed in two experimental models: locomotor activity using actophtometer and skeletal muscle relaxant test by Rota rod apparatus. The ethanolic extract of the C. kyllinga demonstrated depression of the CNS activities in the mice. Phytochemical preliminary screening of the root extract has revealed the presence of flavonoids, carbohydrates, phenolic compounds, steroids and saponin glycoside