9 research outputs found
Activated caspace-3 staining of mouse cardiac sections showing apoptosis in sham, 8 weeks post-AB (AB 8 wks) treated with vehicle control and Mdivi.
<p><b>A</b>) Apoptotic cells are seen as red fluorescent dots (scale bar- 20 µm). <b>C</b>) Activated caspace-3 +ve cells represented in an enlarged area. <b>B</b>) Data represents mean ±SE from n = 6 per group; *p<0.05 was considered significant compared to sham and <sup>#</sup>p<0.05 compared to vehicle treated group.</p
Tunnel assay of mouse cardiac sections staining for apoptosis in sham, 8 weeks post-AB (AB 8 wks) treated with vehicle control and Mdivi.
<p><b>A</b>) Apoptotic cells are seen as green fluorescent dots (scale bar- 10 µm). Positive and negative controls are also represented in this image. <b>B</b>) Data represents mean ±SE from n = 6 per group; *p<0.05 was considered significant compared to sham and <sup>#</sup>p<0.05 compared to vehicle treated group.</p
IHC staining of mouse heart sections with mitochondrial marker antibody MTCO-2 in sham, 8 weeks post-AB (AB 8 wks) treated with vehicle control and Mdivi.
<p><b>A</b>) Expression of MTCO-2 is seen as red fluorescence intensity (scale bar-50 µm). <b>B</b>) Data represents mean ±SE from n = 6 per group; *p<0.05 was considered significant compared to sham and <sup>#</sup>p<0.05 compared to vehicle treated group.</p
IHC staining of heart sections with mitophagy markers LC3 and P62 in sham, 8 weeks post-AB (AB 8 wks) treated with vehicle control and Mdivi.
<p><b>A</b>) Expression of LC3 is seen as green fluorescence intensity and P62 as red fluorescence intensity (scale bar- 10 µm). <b>B</b>) Data represents mean ±SE from n = 6 per group; *p<0.05 was considered significant compared to sham and <sup>#</sup>p<0.05 compared to vehicle treated group.</p
mRNA expression of endostatin and angiostatin primers.
<p><b>A</b>) RT-PCR: mRNAs are amplified using respective primers and the bands were quantified using densitometry. <b>B, C</b>) Bar graphs represent respective mRNA expression over GAPDH expression by RT-PCR and real time PCR assay. Data represents mean ±SE from n = 6 per group; *p<0.05 was considered significant compared to sham and <sup>#</sup>p<0.05 compared to vehicle treated group.</p
mRNA expression of MMP-9 and TIMP-3.
<p>A) mRNAs are amplified using respective primers and the bands were quantified using densitometry. <b>B, C</b>) Bar graphs represent respective mRNA expression over GAPDH expression by RT-PCR and real time PCR assay. Data represents mean ±SE from n = 6 per group; *p<0.05 was considered significant compared to sham and <sup>#</sup>p<0.05 compared to vehicle treated group.</p
Mdivi ameliorates left ventricular dysfunction.
<p><b>A</b>) Changes in left ventricular (LV) function following aortic banding (AB) and the effects of Mdivi treatment, a Drp1 inhibitor. Representative M-mode echocardiography images from sham, sham with Mdivi treatment, AB 8 weeks with vehicle and Mdivi treatment groups. The bar graphs represent LVIDd (left ventricular internal dimension in diastole), LVPWd (left ventricular posterior wall dimension in diastole (<b>B</b>), and %FS (percentage fractional shortening) (<b>C</b>). *p<0.05 compared to sham and <sup>#</sup>p<0.05 compared to vehicle treated group. Data represents mean ±SE from n = 6 per group.</p
Mdivi promotes angiogenesis.
<p><b>A</b>) CD-31-immunohistochemical (IHC) staining of heart and secondarily stained with alexaflour 647 in sham, 8 weeks post-AB (AB 8 wks) treated with vehicle control and Mdivi. The expression of CD31 is seen as red fluorescence intensity (scale bar- 20 µm). <b>C</b>) VEGF-IHC staining of heart sections, secondarily stained with alexaflour 594 in sham, 8 weeks post-AB (AB 8 wks) treated with vehicle control and Mdivi. The expression of VEGF is seen as red fluorescence intensity (scale bar- 50 µm). <b>B, D</b>) Data represents mean ±SE from n = 6 per group; *p<0.05 compared to sham and <sup>#</sup>p<0.05 compared to vehicle treated group.</p