Aircraft state estimation using cameras and passive radar

Multiple target tracking (MTT) is a fundamental task in many application domains. It is a difficult problem to solve in general, so applications make use of domain specific and problem-specific knowledge to approach the problem by solving subtasks separately. This work puts forward a MTT framework (MTTF) which is based on the Bayesian recursive estimator (BRE). The MTTF extends a particle filter (PF) to handle the multiple targets and adds a probabilistic graphical model (PGM) data association stage to compute the mapping from detections to trackers. The MTTF was applied to the problem of passively monitoring airspace. Two applications were built: a passive radar MTT module and a comprehensive visual object tracking (VOT) system. Both applications require a solution to the MTT problem, for which the MTTF was utilized. The VOT system performed well on real data recorded at the University of Cape Town (UCT) as part of this investigation. The system was able to detect and track aircraft flying within the region of interest (ROI). The VOT system consisted of a single camera, an image processing module, the MTTF module and an evaluation module. The world coordinate frame target localization was within ±3.2 km and these results are presented on Google Earth. The image plane target localization has an average reprojection error of ±17.3 pixels. The VOT system achieved an average area under the curve value of 0.77 for all receiver operating characteristic curves. These performance figures are typical over the ±1 hr of video recordings taken from the UCT site. The passive radar application was tested on simulated data. The MTTF module was designed to connect to an existing passive radar system developed by Peralex Electronics Pty Ltd. The MTTF module estimated the number of targets in the scene and localized them within a 2D local world Cartesian coordinate system. The investigations encompass numerous areas of research as well as practical aspects of software engineering and systems design

Central limit theorems for the spectra of classes of random fractals

We discuss the spectral asymptotics of some open subsets of the real line with random fractal boundary and of a random fractal, the continuum random tree. In the case of open subsets with random fractal boundary we establish the existence of the second order term in the asymptotics almost surely and then determine when there will be a central limit theorem which captures the fluctuations around this limit. We will show examples from a class of random fractals generated from Dirichlet distributions as this is a relatively simple setting in which there are sets where there will and will not be a central limit theorem. The Brownian continuum random tree can also be viewed as a random fractal generated by a Dirichlet distribution. The first order term in the spectral asymptotics is known almost surely and here we show that there is a central limit theorem describing the fluctuations about this, though the positivity of the variance arising in the central limit theorem is left open. In both cases these fractals can be described through a general Crump-Mode-Jagers branching process and we exploit this connection to establish our central limit theorems for the higher order terms in the spectral asymptotics. Our main tool is a central limit theorem for such general branching processes which we prove under conditions which are weaker than those previously known

PD-1⁺ Tcf1⁺ CD8⁺ T cells from established chronic infection can form memory while retaining a stableimprint of persistent antigen exposure.

Virus-specific PD1 <sup>+</sup> Tcf1 <sup>+</sup> memory-like CD8 <sup>+</sup> T cells (T <sub>ML</sub> s) maintain the CD8 <sup>+</sup> T cell response during chronic viral infection. However, the fate of these cells following cessation of persistent antigen exposure has been unclear. Here, we find that T <sub>ML</sub> s persist upon transfer into antigen-free hosts and form memory following recall stimulation. Phenotypic, functional, and transcriptome analyses show that T <sub>ML</sub> -derived memory cells resemble those arising in response to acute, resolved infection, but they retain features of chronically stimulated cells, including elevated PD-1 and Tox and reduced cytokine expression. This chronic infection imprint is largely accounted for by constitutive Tox expression. Virus-specific Tcf1 <sup>+</sup> CD8 <sup>+</sup> T cells that persist after clearance of systemic infection also display a chronic infection imprint. Notwithstanding, renewed virus exposure induces a recall response, which controls virus infection in part. Thus, cessation of chronic antigen exposure yields a memory CD8 <sup>+</sup> T cell compartment that reflects prior stimulation

The prominent role of neutrophils during the initial phase of infection by Leishmania parasites.

Neutrophils are rapidly and massively recruited to the site of Leishmania inoculation, where they phagocytose the parasites, some of which are able to survive within these first host cells. Neutrophils can thus provide a transient safe shelter for the parasites, prior to their entry into macrophages where they will replicate. In addition, neutrophils release and synthesize rapidly several factors including cytokines and chemokines. The mechanism involved in their rapid recruitment to the site of parasite inoculation, as well as the putative consequences of their massive presence on the microenvironment of the focus of infection will be discussed in the context of the development of the Leishmania-specific immune response

The Prominent Role of Neutrophils during the Initial Phase of Infection by Leishmania Parasites

Neutrophils are rapidly and massively recruited to the site of Leishmania inoculation, where they phagocytose the parasites, some of which are able to survive within these first host cells. Neutrophils can thus provide a transient safe shelter for the parasites, prior to their entry into macrophages where they will replicate. In addition, neutrophils release and synthesize rapidly several factors including cytokines and chemokines. The mechanism involved in their rapid recruitment to the site of parasite inoculation, as well as the putative consequences of their massive presence on the microenvironment of the focus of infection will be discussed in the context of the development of the Leishmania-specific immune response

Blood pressure from the optical Aktiia Bracelet: a 1-month validation study using an extended ISO81060-2 protocol adapted for a cuffless wrist device.

The objective of this study (NCT04027777) was to assess the accuracy and precision of the Aktiia Bracelet, a CE-marked noninvasive optical blood pressure (BP) monitor worn at the wrist, over a period of 1 month. In this study, participants aged between 21 and 65 years were recruited. The clinical investigation extended the ISO81060-2:2013 standard to the specificities of cuffless devices. Each BP assessment consisted of the simultaneous recording of optical signals with Aktiia Bracelet and double-blinded auscultation by two trained observers in the standard sitting position. The algorithms of Aktiia Bracelet further processed the recorded optical signals to perform a signal quality check and to calculate uncalibrated estimates of systolic BP (SBP) and diastolic BP (DBP). These estimates were transformed into mmHg using a subject-dependent calibration parameter, which was calculated using the first two available reference measurements per subject. Eighty-six participants were included in the analysis. The mean and SD of the differences between Aktiia Bracelet estimates and the reference (ISO81060-2 criterion 1) were 0.46 ± 7.75 mmHg for SBP and 0.39 ± 6.86 mmHg for DBP. The SD of the averaged paired difference per subject (ISO81060-2 criterion 2) were 3.9 mmHg for SBP and 3.6 mmHg for DBP. After initialization and during 1 month, the overall accuracy of Aktiia Bracelet satisfied validation criteria 1 and 2 of ISO81060-2 in the sitting position. The Aktiia Bracelet can be recommended for BP measurement in the adult population

The Cardiopulmonary effect of passive movement

Eleven articles were reviewed on the cardiopulmonary effects of passive movements. These included two articles on theneurological effects of passive movements. Of the eleven articles, four were considered to have level II evidence in accordance with Sackett’s rules of evidence. There was little consensus regarding the rate or duration of passive movements. There were some suggestions that upper limb movement produces a greater ventilatory response than lower limb movement. There was a statistically significant increase (p< 0.05) in minute ventilation when the movement was done at a rate of 40 repetitions per minute or more, but this change may not be clinically significant. Passive movements were not detrimental to neurosurgical patients with a normal or slightly elevated intracranial pressure, although the values of the intracranial pressure were not stated.  The studies were limited in that eight of the eleven had small sample sizes and most studies were conducted using normal subjects. Further studies with higher levels of evidence need to be  conducted to verify any results reported to date in the literature. Studies that are relevant to clinical practice also need to be conducted in populations such as sedated intensive care patients

Transcriptional regulation of murine natural killer cell development, differentiation and maturation.

Natural killer (NK) cells are innate cytotoxic effector cells that play important protective roles against certain pathogens as well as against pathogen-infected and transformed host cells. NK cells continuously arise from adult bone marrow-resident haematopoietic progenitors. Their generation can be sub-divided into three phases. The early NK cell development phase from multipotent common lymphoid progenitors occurs at least in part in common with that of additional members of a family of innate lymphoid cells, for which NK cells are the founding member. An intermediate phase of NK cell differentiation is characterized by the acquisition of IL-15 responsiveness and lineage-defining properties such as the transcription of genes coding for cytotoxic effector molecules. This is followed by a late maturation phase during which NK cells lose homeostatic expansion and increase effector capacity. These three phases are regulated by multiple stage-specific but not NK cell-specific transcription factors. This review summarizes the NK cell developmental and maturation processes and their transcriptional regulation with an emphasis on data derived from genetically modified mouse models