8 research outputs found
Additional file 1: Table S1. of Positional plasticity in regenerating Amybstoma mexicanum limbs is associated with cell proliferation and pathways of cellular differentiation
Probesets with interaction term p < 0.05. (XLSX 227 kb
Additional file 2: Figure S1. of Positional plasticity in regenerating Amybstoma mexicanum limbs is associated with cell proliferation and pathways of cellular differentiation
Selection of 15 clusters of gene expression in EB, apical-LB, and basal-LB in different locations on the P/D limb axis. Join distance (1-Pearson’s correlation, y-axis) plotted as a function of number of clusters. After fifteen clusters, join distances reach an asymptote, suggesting that using more than fifteen groups provides little additional information (small distance indicates separating similarly expressed genes), while using more than fifteen clusters results in joining dissimilar groups (high distance). (TIFF 1203 kb
Additional file 5: Figure S2. of Positional plasticity in regenerating Amybstoma mexicanum limbs is associated with cell proliferation and pathways of cellular differentiation
Workflow of actin quantification on a transverse section of a LB-stage blastema. (A) Confocal image of a transverse section of a LB blastema that had been stained with phalloidin-rhodamin for F-actin (Red). (B) The actin fiber orientations within the blastema mesenchyme of the tissue section was quantified using automated image processing (described in Methods section). Each color represents the orientation of fibers toward a specific direction. For example, red colored fibers are oriented along the proximal/distal axis. (C) The image was divided into small non-overlapping tiles (512 × 512 pixels) each covering approximately 150 square microns of tissue, and all together covered all of the mesenchymal tissue from each section. (D) The discrete entropy of each histogram was computed as a summary statistic to measure the degree of order (alignment) or disorder of actin filaments within the region of tissue spanned by the tile. (E) Histogram representing the average entropy of the tiles from each region of the blastema (Blast/stump, Basal, Mid, Apical, as indicated in (D)). The error bars are the SEM, and Student’s t-test was used to determine statistically signficant changes in organization (p < 0.01). (TIFF 538 kb
BoletÃn de Segovia: Número 15 - 1918 febrero 4
Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 200
Additional file 9: Data 1. of Analysis of in vivo single cell behavior by high throughput, human-in-the-loop segmentation of three-dimensional images
DNA morphologies of cells classified as G1-, S-, G2-, and M-phase based on DNA and EdU content. (PDF 3296 kb
Additional file 7: Figure S6. of Analysis of in vivo single cell behavior by high throughput, human-in-the-loop segmentation of three-dimensional images
Benchmarking of cell row counter accuracy for C. elegans gonadal arms. (A) Overlay of microscope image (white signal, derived from the DNA stain DAPI) with segmentations color-coded by cell row position from the distal end (left), as computed automatically using our counter. (B) Size of the MZ scored manually vs size computed through the automatic counter. A small amount of noise (0.5 cell rows) was added in order to aid visualization of overlapping data. Positions scored manually and automatically are in close agreement; average percent deviation is 9.4 %. Diagonal shown for reference in red. (PDF 152 kb
BoletÃn de Segovia: Número 38 - 1884 marzo 26
Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 200
Additional file 10: Figure S8. of Analysis of in vivo single cell behavior by high throughput, human-in-the-loop segmentation of three-dimensional images
Comparison of frozen or vibratome section segmentations. (A) Active contour segmentation of frozen sections is often inaccurate (red arrows) due to poor cell separation in the original image. (B) Vibratome sections enable clearer staining and thus more accurate segmentation (yellow arrows). (PDF 749 kb